Cooperation of sumoylated chromosomal proteins in rDNA maintenance.
SUMO is a posttranslational modifier that can modulate protein activities, interactions, and localizations. As the GFP-Smt3p fusion protein has a preference for subnucleolar localization, especially when deconjugation is impaired, the nucleolar role of SUMO can be the key to its biological functions...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
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Public Library of Science (PLoS)
2008-10-01
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Series: | PLoS Genetics |
Online Access: | http://europepmc.org/articles/PMC2563031?pdf=render |
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author | Yoshimitsu Takahashi Stanimir Dulev Xianpeng Liu Natalie Jasmin Hiller Xiaolan Zhao Alexander Strunnikov |
author_facet | Yoshimitsu Takahashi Stanimir Dulev Xianpeng Liu Natalie Jasmin Hiller Xiaolan Zhao Alexander Strunnikov |
author_sort | Yoshimitsu Takahashi |
collection | DOAJ |
description | SUMO is a posttranslational modifier that can modulate protein activities, interactions, and localizations. As the GFP-Smt3p fusion protein has a preference for subnucleolar localization, especially when deconjugation is impaired, the nucleolar role of SUMO can be the key to its biological functions. Using conditional triple SUMO E3 mutants, we show that defects in sumoylation impair rDNA maintenance, i.e., the rDNA segregation is defective and the rDNA copy number decreases in these mutants. Upon characterization of sumoylated proteins involved in rDNA maintenance, we established that Top1p and Top2p, which are sumoylated by Siz1p/Siz2p, most likely collaborate with substrates of Mms21p to maintain rDNA integrity. Cohesin and condensin subunits, which both play important roles in rDNA stability and structures, are potential substrates of Mms21, as their sumoylation depends on Mms21p, but not Siz1p and Siz2p. In addition, binding of cohesin and condensin to rDNA is altered in the mms21-CH E3-deficient mutant. |
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institution | Directory Open Access Journal |
issn | 1553-7390 1553-7404 |
language | English |
last_indexed | 2024-12-14T05:10:52Z |
publishDate | 2008-10-01 |
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series | PLoS Genetics |
spelling | doaj.art-f993b86744f14f59ae2bfc8d9216eecc2022-12-21T23:15:58ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042008-10-01410e100021510.1371/journal.pgen.1000215Cooperation of sumoylated chromosomal proteins in rDNA maintenance.Yoshimitsu TakahashiStanimir DulevXianpeng LiuNatalie Jasmin HillerXiaolan ZhaoAlexander StrunnikovSUMO is a posttranslational modifier that can modulate protein activities, interactions, and localizations. As the GFP-Smt3p fusion protein has a preference for subnucleolar localization, especially when deconjugation is impaired, the nucleolar role of SUMO can be the key to its biological functions. Using conditional triple SUMO E3 mutants, we show that defects in sumoylation impair rDNA maintenance, i.e., the rDNA segregation is defective and the rDNA copy number decreases in these mutants. Upon characterization of sumoylated proteins involved in rDNA maintenance, we established that Top1p and Top2p, which are sumoylated by Siz1p/Siz2p, most likely collaborate with substrates of Mms21p to maintain rDNA integrity. Cohesin and condensin subunits, which both play important roles in rDNA stability and structures, are potential substrates of Mms21, as their sumoylation depends on Mms21p, but not Siz1p and Siz2p. In addition, binding of cohesin and condensin to rDNA is altered in the mms21-CH E3-deficient mutant.http://europepmc.org/articles/PMC2563031?pdf=render |
spellingShingle | Yoshimitsu Takahashi Stanimir Dulev Xianpeng Liu Natalie Jasmin Hiller Xiaolan Zhao Alexander Strunnikov Cooperation of sumoylated chromosomal proteins in rDNA maintenance. PLoS Genetics |
title | Cooperation of sumoylated chromosomal proteins in rDNA maintenance. |
title_full | Cooperation of sumoylated chromosomal proteins in rDNA maintenance. |
title_fullStr | Cooperation of sumoylated chromosomal proteins in rDNA maintenance. |
title_full_unstemmed | Cooperation of sumoylated chromosomal proteins in rDNA maintenance. |
title_short | Cooperation of sumoylated chromosomal proteins in rDNA maintenance. |
title_sort | cooperation of sumoylated chromosomal proteins in rdna maintenance |
url | http://europepmc.org/articles/PMC2563031?pdf=render |
work_keys_str_mv | AT yoshimitsutakahashi cooperationofsumoylatedchromosomalproteinsinrdnamaintenance AT stanimirdulev cooperationofsumoylatedchromosomalproteinsinrdnamaintenance AT xianpengliu cooperationofsumoylatedchromosomalproteinsinrdnamaintenance AT nataliejasminhiller cooperationofsumoylatedchromosomalproteinsinrdnamaintenance AT xiaolanzhao cooperationofsumoylatedchromosomalproteinsinrdnamaintenance AT alexanderstrunnikov cooperationofsumoylatedchromosomalproteinsinrdnamaintenance |