Transforming Endothelium with Platelet‐Rich Plasma in Engineered Microvessels
Abstract Vascularization remains an obstacle when engineering complex tissues for regeneration and disease modeling. Although progress has been made in recreating 3D vascular structures, challenges exist in generating a mature, functional endothelium. It is demonstrated that perfusing engineered mic...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-12-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.201901725 |
| _version_ | 1819160065283719168 |
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| author | Ryan J. Nagao Raluca Marcu Yuliang Wang Lu Wang Chris Arakawa Cole DeForest Junmei Chen José A. López Ying Zheng |
| author_facet | Ryan J. Nagao Raluca Marcu Yuliang Wang Lu Wang Chris Arakawa Cole DeForest Junmei Chen José A. López Ying Zheng |
| author_sort | Ryan J. Nagao |
| collection | DOAJ |
| description | Abstract Vascularization remains an obstacle when engineering complex tissues for regeneration and disease modeling. Although progress has been made in recreating 3D vascular structures, challenges exist in generating a mature, functional endothelium. It is demonstrated that perfusing engineered microvessels with platelet‐rich plasma, a critical homeostatic component in vivo that is often overlooked in vitro, substantially transforms the endothelium, both maturing endothelial cells and improving functionality in 24 h. Platelets readily adhered to the exposed collagen‐I substrate through small gaps within engineered vessels without forming thrombi. The adherent platelets improve barrier function, enhance endothelial glycolysis, reduce thrombogenicity, and enrich smooth muscle cell growth surrounding the endothelium. These findings demonstrate that platelets are essential to the function of endothelium during vascular maturation and remodeling. This study sheds light on a potential strategy to engineer stable, implantable vascular networks. |
| first_indexed | 2024-12-22T16:50:31Z |
| format | Article |
| id | doaj.art-f997746739784271a70ad55a245e5d22 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-12-22T16:50:31Z |
| publishDate | 2019-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-f997746739784271a70ad55a245e5d222022-12-21T18:19:36ZengWileyAdvanced Science2198-38442019-12-01624n/an/a10.1002/advs.201901725Transforming Endothelium with Platelet‐Rich Plasma in Engineered MicrovesselsRyan J. Nagao0Raluca Marcu1Yuliang Wang2Lu Wang3Chris Arakawa4Cole DeForest5Junmei Chen6José A. López7Ying Zheng8Department of Bioengineering University of Washington Seattle WA 98195 USADepartment of Bioengineering University of Washington Seattle WA 98195 USADepartment of Computer Science and Engineering University of Washington Seattle WA 98195 USADepartment of Environmental and Occupational Health Sciences University of Washington Seattle WA 98105‐6099 USADepartment of Bioengineering University of Washington Seattle WA 98195 USADepartment of Bioengineering University of Washington Seattle WA 98195 USABloodworks Northwest Research Institute Seattle WA 98102 USADepartment of Chemical Engineering University of Washington Seattle WA 98195 USADepartment of Bioengineering University of Washington Seattle WA 98195 USAAbstract Vascularization remains an obstacle when engineering complex tissues for regeneration and disease modeling. Although progress has been made in recreating 3D vascular structures, challenges exist in generating a mature, functional endothelium. It is demonstrated that perfusing engineered microvessels with platelet‐rich plasma, a critical homeostatic component in vivo that is often overlooked in vitro, substantially transforms the endothelium, both maturing endothelial cells and improving functionality in 24 h. Platelets readily adhered to the exposed collagen‐I substrate through small gaps within engineered vessels without forming thrombi. The adherent platelets improve barrier function, enhance endothelial glycolysis, reduce thrombogenicity, and enrich smooth muscle cell growth surrounding the endothelium. These findings demonstrate that platelets are essential to the function of endothelium during vascular maturation and remodeling. This study sheds light on a potential strategy to engineer stable, implantable vascular networks.https://doi.org/10.1002/advs.201901725endotheliumengineered microvesselspermeabilityplateletsPRP |
| spellingShingle | Ryan J. Nagao Raluca Marcu Yuliang Wang Lu Wang Chris Arakawa Cole DeForest Junmei Chen José A. López Ying Zheng Transforming Endothelium with Platelet‐Rich Plasma in Engineered Microvessels Advanced Science endothelium engineered microvessels permeability platelets PRP |
| title | Transforming Endothelium with Platelet‐Rich Plasma in Engineered Microvessels |
| title_full | Transforming Endothelium with Platelet‐Rich Plasma in Engineered Microvessels |
| title_fullStr | Transforming Endothelium with Platelet‐Rich Plasma in Engineered Microvessels |
| title_full_unstemmed | Transforming Endothelium with Platelet‐Rich Plasma in Engineered Microvessels |
| title_short | Transforming Endothelium with Platelet‐Rich Plasma in Engineered Microvessels |
| title_sort | transforming endothelium with platelet rich plasma in engineered microvessels |
| topic | endothelium engineered microvessels permeability platelets PRP |
| url | https://doi.org/10.1002/advs.201901725 |
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