Potent Hemostatic Efficacy of a Novel Recombinant Fibrin Sealant Patch (KTF-374) in Rabbit Bleeding Models
Purpose: Fibrin sealants are used for hemostasis during surgery. Commercially available fibrin sealants are made of materials of human or animal origin. We developed a novel recombinant fibrin sealant patch (KTF-374) that has thin and flexible properties. This study evaluated the hemostatic efficacy...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-04-01
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| Series: | Journal of Investigative Surgery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08941939.2017.1398789 |
| _version_ | 1797684580050796544 |
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| author | Sumika Miyabashira (Tanaka) Takayuki Imamura Miho Fujimoto Akitoshi Ohno Tsunefumi Kobayashi Noriko Shinya |
| author_facet | Sumika Miyabashira (Tanaka) Takayuki Imamura Miho Fujimoto Akitoshi Ohno Tsunefumi Kobayashi Noriko Shinya |
| author_sort | Sumika Miyabashira (Tanaka) |
| collection | DOAJ |
| description | Purpose: Fibrin sealants are used for hemostasis during surgery. Commercially available fibrin sealants are made of materials of human or animal origin. We developed a novel recombinant fibrin sealant patch (KTF-374) that has thin and flexible properties. This study evaluated the hemostatic efficacy of KTF-374 for various patterns of bleeding in rabbits, as compared with that of the existing fibrin-coated collagen fleece (FCCF). Materials and Methods: Test hemostats used were KTF-374 and FCCF. Laparotomy was performed under general anesthesia in rabbits. We created wounds in the liver, caudal vena cava, and ventral aorta under anticoagulating conditions with heparin. Test hemostats were then applied to the wound site and compressed manually for 3 min. Hemostatic efficacy was evaluated with the success rate of hemostasis at 3 min. Results: In all bleeding models, the success rate of hemostasis was significantly higher with KTF-374 than FCCF. The hemostatic success rate of KTF-374 and FCCF was 100% vs. 25% (p = .007) in the partial hepatectomy model (n = 8); 100% vs. 12.5% (p = .001) in the caudal vena cava resection model (n = 8); and 100% vs. 25% (p = .004) in the ventral aortic puncture model (n = 8). The wound site could clearly be recognized through the patch after the application of KTF-374 but not FCCF. Conclusions: These results suggest that KTF-374 possesses more potent hemostatic properties than FCCF for various patterns of bleeding. KTF-374 is a promising hemostat due to its potent efficacy and good visibility of the wound site through the patch. |
| first_indexed | 2024-03-12T00:31:47Z |
| format | Article |
| id | doaj.art-f9985ad316e74a3f9b0ba954316736f2 |
| institution | Directory Open Access Journal |
| issn | 0894-1939 1521-0553 |
| language | English |
| last_indexed | 2024-03-12T00:31:47Z |
| publishDate | 2019-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj.art-f9985ad316e74a3f9b0ba954316736f22023-09-15T10:12:28ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532019-04-0132325726110.1080/08941939.2017.13987891398789Potent Hemostatic Efficacy of a Novel Recombinant Fibrin Sealant Patch (KTF-374) in Rabbit Bleeding ModelsSumika Miyabashira (Tanaka)0Takayuki Imamura1Miho Fujimoto2Akitoshi Ohno3Tsunefumi Kobayashi4Noriko Shinya5The Chemo-Sero-Therapeutic Research Institute (KAKETSUKEN)The Chemo-Sero-Therapeutic Research Institute (KAKETSUKEN)The Chemo-Sero-Therapeutic Research Institute (KAKETSUKEN)Teijin Institute for Bio-medical Research, Teijin Pharma LimitedTeijin Institute for Bio-medical Research, Teijin Pharma LimitedThe Chemo-Sero-Therapeutic Research Institute (KAKETSUKEN)Purpose: Fibrin sealants are used for hemostasis during surgery. Commercially available fibrin sealants are made of materials of human or animal origin. We developed a novel recombinant fibrin sealant patch (KTF-374) that has thin and flexible properties. This study evaluated the hemostatic efficacy of KTF-374 for various patterns of bleeding in rabbits, as compared with that of the existing fibrin-coated collagen fleece (FCCF). Materials and Methods: Test hemostats used were KTF-374 and FCCF. Laparotomy was performed under general anesthesia in rabbits. We created wounds in the liver, caudal vena cava, and ventral aorta under anticoagulating conditions with heparin. Test hemostats were then applied to the wound site and compressed manually for 3 min. Hemostatic efficacy was evaluated with the success rate of hemostasis at 3 min. Results: In all bleeding models, the success rate of hemostasis was significantly higher with KTF-374 than FCCF. The hemostatic success rate of KTF-374 and FCCF was 100% vs. 25% (p = .007) in the partial hepatectomy model (n = 8); 100% vs. 12.5% (p = .001) in the caudal vena cava resection model (n = 8); and 100% vs. 25% (p = .004) in the ventral aortic puncture model (n = 8). The wound site could clearly be recognized through the patch after the application of KTF-374 but not FCCF. Conclusions: These results suggest that KTF-374 possesses more potent hemostatic properties than FCCF for various patterns of bleeding. KTF-374 is a promising hemostat due to its potent efficacy and good visibility of the wound site through the patch.http://dx.doi.org/10.1080/08941939.2017.1398789fibrin sealantshemostatrabbit bleeding modelsrecombinant fibrinogen/thrombin patch |
| spellingShingle | Sumika Miyabashira (Tanaka) Takayuki Imamura Miho Fujimoto Akitoshi Ohno Tsunefumi Kobayashi Noriko Shinya Potent Hemostatic Efficacy of a Novel Recombinant Fibrin Sealant Patch (KTF-374) in Rabbit Bleeding Models Journal of Investigative Surgery fibrin sealants hemostat rabbit bleeding models recombinant fibrinogen/thrombin patch |
| title | Potent Hemostatic Efficacy of a Novel Recombinant Fibrin Sealant Patch (KTF-374) in Rabbit Bleeding Models |
| title_full | Potent Hemostatic Efficacy of a Novel Recombinant Fibrin Sealant Patch (KTF-374) in Rabbit Bleeding Models |
| title_fullStr | Potent Hemostatic Efficacy of a Novel Recombinant Fibrin Sealant Patch (KTF-374) in Rabbit Bleeding Models |
| title_full_unstemmed | Potent Hemostatic Efficacy of a Novel Recombinant Fibrin Sealant Patch (KTF-374) in Rabbit Bleeding Models |
| title_short | Potent Hemostatic Efficacy of a Novel Recombinant Fibrin Sealant Patch (KTF-374) in Rabbit Bleeding Models |
| title_sort | potent hemostatic efficacy of a novel recombinant fibrin sealant patch ktf 374 in rabbit bleeding models |
| topic | fibrin sealants hemostat rabbit bleeding models recombinant fibrinogen/thrombin patch |
| url | http://dx.doi.org/10.1080/08941939.2017.1398789 |
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