New Insights into the Role of HMGB2 in ST-Segment Elevation Myocardial Infarction
Hao Qin,1,* Wenjun Wang,2,* Longlong Hu,1 Zuozhong Yu,1 Yang Chen,1 Yuanbin Zhao,1 Yanhui Liao,1 Renqiang Yang1 1Department of Cardiovascular Medicine, the Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, People’s Republic of China; 2Department of Respira...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-09-01
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| Series: | International Journal of General Medicine |
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| Online Access: | https://www.dovepress.com/new-insights-into-the-role-of-hmgb2-in-st-segment-elevation-myocardial-peer-reviewed-fulltext-article-IJGM |
| _version_ | 1827818286243381248 |
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| author | Qin H Wang W Hu L Yu Z Chen Y Zhao Y Liao Y Yang R |
| author_facet | Qin H Wang W Hu L Yu Z Chen Y Zhao Y Liao Y Yang R |
| author_sort | Qin H |
| collection | DOAJ |
| description | Hao Qin,1,* Wenjun Wang,2,* Longlong Hu,1 Zuozhong Yu,1 Yang Chen,1 Yuanbin Zhao,1 Yanhui Liao,1 Renqiang Yang1 1Department of Cardiovascular Medicine, the Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, People’s Republic of China; 2Department of Respiratory Diseases, the Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, People’s Republic of China*These authors contributed equally to this workCorrespondence: Renqiang Yang, Department of Cardiovascular Medicine, the Second Affiliated Hospital of Nanchang University, 1 Minde Road, Nanchang, Jiangxi, 330006, People’s Republic of China, Tel +86-13979117790, Fax +86-0791-87357375, Email yangrenqiangcn@163.comBackground: Ischemic heart disease is one of the leading causes of death in the world, of which ST-segment elevation myocardial infarction (STEMI) is an important type. Inappropriate activation and accumulation of platelets typically induced thrombosis, which may result in acute vessel occlusion and STEMI. Multiple cytokines have been shown to regulate platelet activation, but the relationship between HMGB2 and platelet activation has not been elucidated.Methods: We collected peripheral blood of STEMI patients and healthy adults, and mass spectrometry analysis of platelet proteins was conducted. The “edgeR” package was used to identify the differentially expressed proteins (DEPs). The Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene ontology (GO) and Gene Set Enrichment Analysis (GSEA) were used to identify the significantly changed pathways. Western blot and ELISA were used to detect the expression of a high mobility group box 2 (HMGB2). Flow cytometric analysis and platelet aggregation rate were performed to evaluate the activation of platelets.Results: We identified ALOX5, HIST1H1B, S100A11, HMGB2, and RPS15A were the top five up-regulated proteins by differential expression analysis. Western blot verified that the relative protein expression of HMGB2 in platelet was significantly higher in STEMI patients compared with control adults, and the results of ELISA indicated that the serum HMGB2 level increased and significantly correlated with neutrophil count in STEMI patients. Further investigation showed that the platelet aggregation induced by ADP, the activation of integrin αIIbβ 3 and CD62P expression on platelet surface were all enhanced by the recombinant HMGB2 (rHMGB2).Conclusion: In conclusion, HMGB2 may be the key molecule to regulate platelet activation in patients with STEMI, which may serve as a potential therapeutic target for STEMI.Keywords: ST-segment elevation myocardial infarction, STEMI, proteomics analysis, platelet, HMGB2 |
| first_indexed | 2024-03-12T00:45:41Z |
| format | Article |
| id | doaj.art-f999fa0f353749c79d6b7ecc059926a8 |
| institution | Directory Open Access Journal |
| issn | 1178-7074 |
| language | English |
| last_indexed | 2024-03-12T00:45:41Z |
| publishDate | 2023-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of General Medicine |
| spelling | doaj.art-f999fa0f353749c79d6b7ecc059926a82023-09-14T19:09:02ZengDove Medical PressInternational Journal of General Medicine1178-70742023-09-01Volume 164181419186688New Insights into the Role of HMGB2 in ST-Segment Elevation Myocardial InfarctionQin HWang WHu LYu ZChen YZhao YLiao YYang RHao Qin,1,* Wenjun Wang,2,* Longlong Hu,1 Zuozhong Yu,1 Yang Chen,1 Yuanbin Zhao,1 Yanhui Liao,1 Renqiang Yang1 1Department of Cardiovascular Medicine, the Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, People’s Republic of China; 2Department of Respiratory Diseases, the Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, People’s Republic of China*These authors contributed equally to this workCorrespondence: Renqiang Yang, Department of Cardiovascular Medicine, the Second Affiliated Hospital of Nanchang University, 1 Minde Road, Nanchang, Jiangxi, 330006, People’s Republic of China, Tel +86-13979117790, Fax +86-0791-87357375, Email yangrenqiangcn@163.comBackground: Ischemic heart disease is one of the leading causes of death in the world, of which ST-segment elevation myocardial infarction (STEMI) is an important type. Inappropriate activation and accumulation of platelets typically induced thrombosis, which may result in acute vessel occlusion and STEMI. Multiple cytokines have been shown to regulate platelet activation, but the relationship between HMGB2 and platelet activation has not been elucidated.Methods: We collected peripheral blood of STEMI patients and healthy adults, and mass spectrometry analysis of platelet proteins was conducted. The “edgeR” package was used to identify the differentially expressed proteins (DEPs). The Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene ontology (GO) and Gene Set Enrichment Analysis (GSEA) were used to identify the significantly changed pathways. Western blot and ELISA were used to detect the expression of a high mobility group box 2 (HMGB2). Flow cytometric analysis and platelet aggregation rate were performed to evaluate the activation of platelets.Results: We identified ALOX5, HIST1H1B, S100A11, HMGB2, and RPS15A were the top five up-regulated proteins by differential expression analysis. Western blot verified that the relative protein expression of HMGB2 in platelet was significantly higher in STEMI patients compared with control adults, and the results of ELISA indicated that the serum HMGB2 level increased and significantly correlated with neutrophil count in STEMI patients. Further investigation showed that the platelet aggregation induced by ADP, the activation of integrin αIIbβ 3 and CD62P expression on platelet surface were all enhanced by the recombinant HMGB2 (rHMGB2).Conclusion: In conclusion, HMGB2 may be the key molecule to regulate platelet activation in patients with STEMI, which may serve as a potential therapeutic target for STEMI.Keywords: ST-segment elevation myocardial infarction, STEMI, proteomics analysis, platelet, HMGB2https://www.dovepress.com/new-insights-into-the-role-of-hmgb2-in-st-segment-elevation-myocardial-peer-reviewed-fulltext-article-IJGMst-segment elevation myocardial infarctionstemiproteomics analysisplatelethmgb2 |
| spellingShingle | Qin H Wang W Hu L Yu Z Chen Y Zhao Y Liao Y Yang R New Insights into the Role of HMGB2 in ST-Segment Elevation Myocardial Infarction International Journal of General Medicine st-segment elevation myocardial infarction stemi proteomics analysis platelet hmgb2 |
| title | New Insights into the Role of HMGB2 in ST-Segment Elevation Myocardial Infarction |
| title_full | New Insights into the Role of HMGB2 in ST-Segment Elevation Myocardial Infarction |
| title_fullStr | New Insights into the Role of HMGB2 in ST-Segment Elevation Myocardial Infarction |
| title_full_unstemmed | New Insights into the Role of HMGB2 in ST-Segment Elevation Myocardial Infarction |
| title_short | New Insights into the Role of HMGB2 in ST-Segment Elevation Myocardial Infarction |
| title_sort | new insights into the role of hmgb2 in st segment elevation myocardial infarction |
| topic | st-segment elevation myocardial infarction stemi proteomics analysis platelet hmgb2 |
| url | https://www.dovepress.com/new-insights-into-the-role-of-hmgb2-in-st-segment-elevation-myocardial-peer-reviewed-fulltext-article-IJGM |
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