A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples
Chlorantraniliprole (CAP) is a new type of diamide insecticide that is mainly used to control lepidopteran pests. However, it has been proven to be hazardous to nontarget organisms, and the effects of its residues need to be monitored. In this study, five hybridoma cell lines were developed that pro...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/2/205 |
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| author | Yanling Wu Jiao Li Jie Zhu Zhaoxian Zhang Shuguang Zhang Minghua Wang Xiude Hua |
| author_facet | Yanling Wu Jiao Li Jie Zhu Zhaoxian Zhang Shuguang Zhang Minghua Wang Xiude Hua |
| author_sort | Yanling Wu |
| collection | DOAJ |
| description | Chlorantraniliprole (CAP) is a new type of diamide insecticide that is mainly used to control lepidopteran pests. However, it has been proven to be hazardous to nontarget organisms, and the effects of its residues need to be monitored. In this study, five hybridoma cell lines were developed that produced anti-CAP monoclonal antibodies (mAbs), of which the mAb originating from the cell line 5C5B9 showed the highest sensitivity and was used to develop a gold nanoparticle-based lateral flow immunoassay (AuNP-LFIA) for CAP. The visible limit of detection of the AuNP-LFIA was 1.25 ng/mL, and the detection results were obtained in less than 10 min. The AuNP-LFIA showed no cross-reactivity for CAP analogs, except for tetraniliprole (50%) and cyclaniliprole (5%). In the detection of spiked and blind samples, the accuracy and reliability of the AuNP-LFIA were confirmed by a comparison with spiked concentrations and verified by ultra-performance liquid chromatography–tandem mass spectrometry. Thus, this study provides the core reagents for establishing CAP immunoassays and a AuNP-LFIA for the detection of residual CAP. |
| first_indexed | 2024-03-08T09:55:25Z |
| format | Article |
| id | doaj.art-f99a7570a3b24bd7981e7ff156fe2af9 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-08T09:55:25Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-f99a7570a3b24bd7981e7ff156fe2af92024-01-29T13:51:51ZengMDPI AGFoods2304-81582024-01-0113220510.3390/foods13020205A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental SamplesYanling Wu0Jiao Li1Jie Zhu2Zhaoxian Zhang3Shuguang Zhang4Minghua Wang5Xiude Hua6College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Agri-Food Safety of Anhui Province, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, ChinaCollege of Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaChlorantraniliprole (CAP) is a new type of diamide insecticide that is mainly used to control lepidopteran pests. However, it has been proven to be hazardous to nontarget organisms, and the effects of its residues need to be monitored. In this study, five hybridoma cell lines were developed that produced anti-CAP monoclonal antibodies (mAbs), of which the mAb originating from the cell line 5C5B9 showed the highest sensitivity and was used to develop a gold nanoparticle-based lateral flow immunoassay (AuNP-LFIA) for CAP. The visible limit of detection of the AuNP-LFIA was 1.25 ng/mL, and the detection results were obtained in less than 10 min. The AuNP-LFIA showed no cross-reactivity for CAP analogs, except for tetraniliprole (50%) and cyclaniliprole (5%). In the detection of spiked and blind samples, the accuracy and reliability of the AuNP-LFIA were confirmed by a comparison with spiked concentrations and verified by ultra-performance liquid chromatography–tandem mass spectrometry. Thus, this study provides the core reagents for establishing CAP immunoassays and a AuNP-LFIA for the detection of residual CAP.https://www.mdpi.com/2304-8158/13/2/205chlorantraniliprolemonoclonal antibodylateral flow immunoassaygold nanoparticlepesticide residue |
| spellingShingle | Yanling Wu Jiao Li Jie Zhu Zhaoxian Zhang Shuguang Zhang Minghua Wang Xiude Hua A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples Foods chlorantraniliprole monoclonal antibody lateral flow immunoassay gold nanoparticle pesticide residue |
| title | A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples |
| title_full | A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples |
| title_fullStr | A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples |
| title_full_unstemmed | A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples |
| title_short | A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples |
| title_sort | rapid and sensitive gold nanoparticle based lateral flow immunoassay for chlorantraniliprole in agricultural and environmental samples |
| topic | chlorantraniliprole monoclonal antibody lateral flow immunoassay gold nanoparticle pesticide residue |
| url | https://www.mdpi.com/2304-8158/13/2/205 |
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