Power of a dual‐use SNP panel for pedigree reconstruction and population assignment
Abstract The use of high‐throughput, low‐density sequencing approaches has dramatically increased in recent years in studies of eco‐evolutionary processes in wild populations and domestication in commercial aquaculture. Most of these studies focus on identifying panels of SNP loci for a single downs...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-09-01
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| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.6645 |
| _version_ | 1828909198139719680 |
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| author | Samuel A. May Garrett J. McKinney Ray Hilborn Lorenz Hauser Kerry A. Naish |
| author_facet | Samuel A. May Garrett J. McKinney Ray Hilborn Lorenz Hauser Kerry A. Naish |
| author_sort | Samuel A. May |
| collection | DOAJ |
| description | Abstract The use of high‐throughput, low‐density sequencing approaches has dramatically increased in recent years in studies of eco‐evolutionary processes in wild populations and domestication in commercial aquaculture. Most of these studies focus on identifying panels of SNP loci for a single downstream application, whereas there have been few studies examining the trade‐offs for selecting panels of markers for use in multiple applications. Here, we detail the use of a bioinformatic workflow for the development of a dual‐purpose SNP panel for parentage and population assignment, which included identifying putative SNP loci, filtering for the most informative loci for the two tasks, designing effective multiplex PCR primers, optimizing the SNP panel for performance, and performing quality control steps for downstream applications. We applied this workflow to two adjacent Alaskan Sockeye Salmon populations and identified a GTseq panel of 142 SNP loci for parentage and 35 SNP loci for population assignment. Only 50–75 panel loci were necessary for >95% accurate parentage, whereas population assignment success, with all 172 panel loci, ranged from 93.9% to 96.2%. Finally, we discuss the trade‐offs and complexities of the decision‐making process that drives SNP panel development, optimization, and testing. |
| first_indexed | 2024-12-13T18:22:19Z |
| format | Article |
| id | doaj.art-f99bd55de3a047f98b1255a62f2932cb |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-12-13T18:22:19Z |
| publishDate | 2020-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-f99bd55de3a047f98b1255a62f2932cb2022-12-21T23:35:41ZengWileyEcology and Evolution2045-77582020-09-0110179522953110.1002/ece3.6645Power of a dual‐use SNP panel for pedigree reconstruction and population assignmentSamuel A. May0Garrett J. McKinney1Ray Hilborn2Lorenz Hauser3Kerry A. Naish4School of Aquatic and Fishery Sciences University of Washington Seattle WA USANRC Research Associateship Program Northwest Fisheries Science Center National Marine Fisheries Service National Oceanic and Atmospheric Administration Seattle WA USASchool of Aquatic and Fishery Sciences University of Washington Seattle WA USASchool of Aquatic and Fishery Sciences University of Washington Seattle WA USASchool of Aquatic and Fishery Sciences University of Washington Seattle WA USAAbstract The use of high‐throughput, low‐density sequencing approaches has dramatically increased in recent years in studies of eco‐evolutionary processes in wild populations and domestication in commercial aquaculture. Most of these studies focus on identifying panels of SNP loci for a single downstream application, whereas there have been few studies examining the trade‐offs for selecting panels of markers for use in multiple applications. Here, we detail the use of a bioinformatic workflow for the development of a dual‐purpose SNP panel for parentage and population assignment, which included identifying putative SNP loci, filtering for the most informative loci for the two tasks, designing effective multiplex PCR primers, optimizing the SNP panel for performance, and performing quality control steps for downstream applications. We applied this workflow to two adjacent Alaskan Sockeye Salmon populations and identified a GTseq panel of 142 SNP loci for parentage and 35 SNP loci for population assignment. Only 50–75 panel loci were necessary for >95% accurate parentage, whereas population assignment success, with all 172 panel loci, ranged from 93.9% to 96.2%. Finally, we discuss the trade‐offs and complexities of the decision‐making process that drives SNP panel development, optimization, and testing.https://doi.org/10.1002/ece3.6645amplicon panelGTseqparentagepopulation assignmentSockeye |
| spellingShingle | Samuel A. May Garrett J. McKinney Ray Hilborn Lorenz Hauser Kerry A. Naish Power of a dual‐use SNP panel for pedigree reconstruction and population assignment Ecology and Evolution amplicon panel GTseq parentage population assignment Sockeye |
| title | Power of a dual‐use SNP panel for pedigree reconstruction and population assignment |
| title_full | Power of a dual‐use SNP panel for pedigree reconstruction and population assignment |
| title_fullStr | Power of a dual‐use SNP panel for pedigree reconstruction and population assignment |
| title_full_unstemmed | Power of a dual‐use SNP panel for pedigree reconstruction and population assignment |
| title_short | Power of a dual‐use SNP panel for pedigree reconstruction and population assignment |
| title_sort | power of a dual use snp panel for pedigree reconstruction and population assignment |
| topic | amplicon panel GTseq parentage population assignment Sockeye |
| url | https://doi.org/10.1002/ece3.6645 |
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