A novel nucleotide insertion in S gene of hepatitis B virus in a chronic carrier
<p>Abstract</p> <p>Hepatitis B virus DNA was extracted from serum of a chronic carrier and polymerase chain reaction was performed on S gene. Direct sequencing showed a variant HBsAg with additional 4-amino acid insertion, and clone sequencing confirmed the mixture of variant HBsAg...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-05-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/7/1/104 |
| _version_ | 1828850019746185216 |
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| author | He Shusen He Jilan Sun Li Tong Wenbin Du Fei |
| author_facet | He Shusen He Jilan Sun Li Tong Wenbin Du Fei |
| author_sort | He Shusen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Hepatitis B virus DNA was extracted from serum of a chronic carrier and polymerase chain reaction was performed on S gene. Direct sequencing showed a variant HBsAg with additional 4-amino acid insertion, and clone sequencing confirmed the mixture of variant HBsAg and wildtype HBsAg. Of 16 clones with 12-nucleotide insertion, 15 clones had identical AGAACAACACAA insertion between nucleotide 494 and nucleotide 495, and one clone had GGAACAACTCAA insertion in the same position plus 3-nucleotide deletion from nucleotide 491 to nucleotide 493. S114T, C121Y, T126S/A, Q129K, G130R, T131N, M133T, G145R, N146D substitution and premature stop codon were also found in those clones. However, the origin of HBV with 4-amino acid insertion in HBsAg was unclear.</p> |
| first_indexed | 2024-12-12T23:03:30Z |
| format | Article |
| id | doaj.art-f9a030a6dc2f4cd5ad7d6c5bfe9dec13 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-12T23:03:30Z |
| publishDate | 2010-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-f9a030a6dc2f4cd5ad7d6c5bfe9dec132022-12-22T00:08:46ZengBMCVirology Journal1743-422X2010-05-017110410.1186/1743-422X-7-104A novel nucleotide insertion in S gene of hepatitis B virus in a chronic carrierHe ShusenHe JilanSun LiTong WenbinDu Fei<p>Abstract</p> <p>Hepatitis B virus DNA was extracted from serum of a chronic carrier and polymerase chain reaction was performed on S gene. Direct sequencing showed a variant HBsAg with additional 4-amino acid insertion, and clone sequencing confirmed the mixture of variant HBsAg and wildtype HBsAg. Of 16 clones with 12-nucleotide insertion, 15 clones had identical AGAACAACACAA insertion between nucleotide 494 and nucleotide 495, and one clone had GGAACAACTCAA insertion in the same position plus 3-nucleotide deletion from nucleotide 491 to nucleotide 493. S114T, C121Y, T126S/A, Q129K, G130R, T131N, M133T, G145R, N146D substitution and premature stop codon were also found in those clones. However, the origin of HBV with 4-amino acid insertion in HBsAg was unclear.</p>http://www.virologyj.com/content/7/1/104 |
| spellingShingle | He Shusen He Jilan Sun Li Tong Wenbin Du Fei A novel nucleotide insertion in S gene of hepatitis B virus in a chronic carrier Virology Journal |
| title | A novel nucleotide insertion in S gene of hepatitis B virus in a chronic carrier |
| title_full | A novel nucleotide insertion in S gene of hepatitis B virus in a chronic carrier |
| title_fullStr | A novel nucleotide insertion in S gene of hepatitis B virus in a chronic carrier |
| title_full_unstemmed | A novel nucleotide insertion in S gene of hepatitis B virus in a chronic carrier |
| title_short | A novel nucleotide insertion in S gene of hepatitis B virus in a chronic carrier |
| title_sort | novel nucleotide insertion in s gene of hepatitis b virus in a chronic carrier |
| url | http://www.virologyj.com/content/7/1/104 |
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