Distribution of bone morphogenetic protein receptors in human scleral fibroblasts cultured in vitro and human sclera
AIM: To investigate the distribution of bone morphogenetic protein receptors (BMPRs) in human scleral fibroblsasts (HSFs) and in human sclera. METHODS: Primary HSFs were cultured in vitro. The mRNA levels of BMP-2 and BMPRs in HSFs were assayed by reverse transcription-polymerase chain reaction (RT-...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2012-12-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530804/ |
| _version_ | 1828516017690640384 |
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| author | Zhen-Ya Gao Li-Jun Huo Dong-Mei Cui Xiao Yang Wen-Juan Wan Jun-Wen Zeng |
| author_facet | Zhen-Ya Gao Li-Jun Huo Dong-Mei Cui Xiao Yang Wen-Juan Wan Jun-Wen Zeng |
| author_sort | Zhen-Ya Gao |
| collection | DOAJ |
| description | AIM: To investigate the distribution of bone morphogenetic protein receptors (BMPRs) in human scleral fibroblsasts (HSFs) and in human sclera. METHODS: Primary HSFs were cultured in vitro. The mRNA levels of BMP-2 and BMPRs in HSFs were assayed by reverse transcription-polymerase chain reaction (RT-PCR). The protein distributions of BMP-2 and BMPRs in HSFs were further detected by immunocytofluorescence and western blot. Their protein expression was also detected in frozen human posterior scleral sections by immunohistofluorescence.RESULTS: BMP-2 and BMPRs were expressed in both HSFs and human sclera not only at mRNA level but also at protein level. The expressions of BMPRIA and BMPRII were higher than that of BMPRIB in the cytoplasm and cell membrane of HSFs in vitro. Western blot further verified the results of immunocytofluorescence. In human sclera, BMP2, BMPR IB and BMPR II were found to be expressed in the cytomatrix of HSF, and weak signal was detected about BMPRIA.CONCLUSION: BMP-2 and all three subtypes of BMPRs were found in HSFs and may play a role in scleral remodeling. |
| first_indexed | 2024-12-11T18:21:11Z |
| format | Article |
| id | doaj.art-f9a11bd43cc74137b95c9f434f32b4e5 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-11T18:21:11Z |
| publishDate | 2012-12-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-f9a11bd43cc74137b95c9f434f32b4e52022-12-22T00:55:15ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982012-12-015666166610.3980/j.issn.2222-3959.2012.06.02Distribution of bone morphogenetic protein receptors in human scleral fibroblasts cultured in vitro and human scleraZhen-Ya GaoLi-Jun HuoDong-Mei CuiXiao YangWen-Juan WanJun-Wen ZengAIM: To investigate the distribution of bone morphogenetic protein receptors (BMPRs) in human scleral fibroblsasts (HSFs) and in human sclera. METHODS: Primary HSFs were cultured in vitro. The mRNA levels of BMP-2 and BMPRs in HSFs were assayed by reverse transcription-polymerase chain reaction (RT-PCR). The protein distributions of BMP-2 and BMPRs in HSFs were further detected by immunocytofluorescence and western blot. Their protein expression was also detected in frozen human posterior scleral sections by immunohistofluorescence.RESULTS: BMP-2 and BMPRs were expressed in both HSFs and human sclera not only at mRNA level but also at protein level. The expressions of BMPRIA and BMPRII were higher than that of BMPRIB in the cytoplasm and cell membrane of HSFs in vitro. Western blot further verified the results of immunocytofluorescence. In human sclera, BMP2, BMPR IB and BMPR II were found to be expressed in the cytomatrix of HSF, and weak signal was detected about BMPRIA.CONCLUSION: BMP-2 and all three subtypes of BMPRs were found in HSFs and may play a role in scleral remodeling.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530804/human scleral fibroblastsBMP-2BMPRs |
| spellingShingle | Zhen-Ya Gao Li-Jun Huo Dong-Mei Cui Xiao Yang Wen-Juan Wan Jun-Wen Zeng Distribution of bone morphogenetic protein receptors in human scleral fibroblasts cultured in vitro and human sclera International Journal of Ophthalmology human scleral fibroblasts BMP-2 BMPRs |
| title | Distribution of bone morphogenetic protein receptors in human scleral fibroblasts cultured in vitro and human sclera |
| title_full | Distribution of bone morphogenetic protein receptors in human scleral fibroblasts cultured in vitro and human sclera |
| title_fullStr | Distribution of bone morphogenetic protein receptors in human scleral fibroblasts cultured in vitro and human sclera |
| title_full_unstemmed | Distribution of bone morphogenetic protein receptors in human scleral fibroblasts cultured in vitro and human sclera |
| title_short | Distribution of bone morphogenetic protein receptors in human scleral fibroblasts cultured in vitro and human sclera |
| title_sort | distribution of bone morphogenetic protein receptors in human scleral fibroblasts cultured in vitro and human sclera |
| topic | human scleral fibroblasts BMP-2 BMPRs |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530804/ |
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