Development and Applications of CRISPR/Cas9-Based Genome Editing in <i>Lactobacillus</i>
<i>Lactobacillus</i>, a genus of lactic acid bacteria, plays a crucial function in food production preservation, and probiotics. It is particularly important to develop new <i>Lactobacillus</i> strains with superior performance by gene editing. Currently, the identification o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/12852 |
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| author | Yulin Mu Chengxiao Zhang Taihua Li Feng-Jie Jin Yun-Ju Sung Hee-Mock Oh Hyung-Gwan Lee Long Jin |
| author_facet | Yulin Mu Chengxiao Zhang Taihua Li Feng-Jie Jin Yun-Ju Sung Hee-Mock Oh Hyung-Gwan Lee Long Jin |
| author_sort | Yulin Mu |
| collection | DOAJ |
| description | <i>Lactobacillus</i>, a genus of lactic acid bacteria, plays a crucial function in food production preservation, and probiotics. It is particularly important to develop new <i>Lactobacillus</i> strains with superior performance by gene editing. Currently, the identification of its functional genes and the mining of excellent functional genes mainly rely on the traditional gene homologous recombination technology. CRISPR/Cas9-based genome editing is a rapidly developing technology in recent years. It has been widely applied in mammalian cells, plants, yeast, and other eukaryotes, but less in prokaryotes, especially <i>Lactobacillus</i>. Compared with the traditional strain improvement methods, CRISPR/Cas9-based genome editing can greatly improve the accuracy of <i>Lactobacillus</i> target sites and achieve traceless genome modification. The strains obtained by this technology may even be more efficient than the traditional random mutation methods. This review examines the application and current issues of CRISPR/Cas9-based genome editing in <i>Lactobacillus</i>, as well as the development trend of CRISPR/Cas9-based genome editing in <i>Lactobacillus</i>. In addition, the fundamental mechanisms of CRISPR/Cas9-based genome editing are also presented and summarized. |
| first_indexed | 2024-03-09T19:02:07Z |
| format | Article |
| id | doaj.art-f9a2a6c1226341ffb236273d96888a7d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:02:07Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f9a2a6c1226341ffb236273d96888a7d2023-11-24T04:58:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123211285210.3390/ijms232112852Development and Applications of CRISPR/Cas9-Based Genome Editing in <i>Lactobacillus</i>Yulin Mu0Chengxiao Zhang1Taihua Li2Feng-Jie Jin3Yun-Ju Sung4Hee-Mock Oh5Hyung-Gwan Lee6Long Jin7College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaBioNanotechnology Research Centre, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, KoreaCell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, KoreaCell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, KoreaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China<i>Lactobacillus</i>, a genus of lactic acid bacteria, plays a crucial function in food production preservation, and probiotics. It is particularly important to develop new <i>Lactobacillus</i> strains with superior performance by gene editing. Currently, the identification of its functional genes and the mining of excellent functional genes mainly rely on the traditional gene homologous recombination technology. CRISPR/Cas9-based genome editing is a rapidly developing technology in recent years. It has been widely applied in mammalian cells, plants, yeast, and other eukaryotes, but less in prokaryotes, especially <i>Lactobacillus</i>. Compared with the traditional strain improvement methods, CRISPR/Cas9-based genome editing can greatly improve the accuracy of <i>Lactobacillus</i> target sites and achieve traceless genome modification. The strains obtained by this technology may even be more efficient than the traditional random mutation methods. This review examines the application and current issues of CRISPR/Cas9-based genome editing in <i>Lactobacillus</i>, as well as the development trend of CRISPR/Cas9-based genome editing in <i>Lactobacillus</i>. In addition, the fundamental mechanisms of CRISPR/Cas9-based genome editing are also presented and summarized.https://www.mdpi.com/1422-0067/23/21/12852CRISPR/Cas9Cas9 protein<i>Lactobacillus</i>genome editing |
| spellingShingle | Yulin Mu Chengxiao Zhang Taihua Li Feng-Jie Jin Yun-Ju Sung Hee-Mock Oh Hyung-Gwan Lee Long Jin Development and Applications of CRISPR/Cas9-Based Genome Editing in <i>Lactobacillus</i> International Journal of Molecular Sciences CRISPR/Cas9 Cas9 protein <i>Lactobacillus</i> genome editing |
| title | Development and Applications of CRISPR/Cas9-Based Genome Editing in <i>Lactobacillus</i> |
| title_full | Development and Applications of CRISPR/Cas9-Based Genome Editing in <i>Lactobacillus</i> |
| title_fullStr | Development and Applications of CRISPR/Cas9-Based Genome Editing in <i>Lactobacillus</i> |
| title_full_unstemmed | Development and Applications of CRISPR/Cas9-Based Genome Editing in <i>Lactobacillus</i> |
| title_short | Development and Applications of CRISPR/Cas9-Based Genome Editing in <i>Lactobacillus</i> |
| title_sort | development and applications of crispr cas9 based genome editing in i lactobacillus i |
| topic | CRISPR/Cas9 Cas9 protein <i>Lactobacillus</i> genome editing |
| url | https://www.mdpi.com/1422-0067/23/21/12852 |
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