Evaluation of Gene Expression Alterations of ATM, TP53, and POLM in Gastric Biopsy Specimens of Patients with Gastritis and its Association with Helicobacter Pylori Infection
Introduction: Gastritis is one of the most common diseases affecting the stomach. Helicobacter pylori infection could lead to DNA damage in gastric epithelial cells, followed by error-prone DNA repair pathways that increase the accumulation of damage at the site of DNA double-stranded breaks, exacer...
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| Format: | Article |
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Ilam University of Medical Sciences
2021-03-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| Subjects: | |
| Online Access: | http://sjimu.medilam.ac.ir/article-1-6713-en.html |
| _version_ | 1818385849232916480 |
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| author | Armin Ghameshloo Ali Rashidi-nezhad Masoud Alebouyeh Abas Shakouri |
| author_facet | Armin Ghameshloo Ali Rashidi-nezhad Masoud Alebouyeh Abas Shakouri |
| author_sort | Armin Ghameshloo |
| collection | DOAJ |
| description | Introduction: Gastritis is one of the most common diseases affecting the stomach. Helicobacter pylori infection could lead to DNA damage in gastric epithelial cells, followed by error-prone DNA repair pathways that increase the accumulation of damage at the site of DNA double-stranded breaks, exacerbate genome instability, and facilitate the emergence of gastric cancer. This study aimed to examine the expression level of ATM, POLM, and TP53 genes involving in the DNA Damage Response and the cell cycle arrest in the gastric precancerous stage.
Materials & Methods: Among 180 gastric biopsy specimens, 30 samples taken from patients with moderate chronic gastritis infected by H. Pylori were regarded as the case group, and 30 other samples taken from non-infected patients with mild chronic gastritis were regarded as control. Following that, RNA extraction and cDNA synthesis was performed. Afterward, the expression levels of ATM, POLM, and TP53 genes were evaluated by the Real-Time PCR method.
Ethics code: 98-02-27-43392
Findings: The ATM, POLM, and TP53 genes in the cases showed a 4.07, 3.35, and 5.13 increase in the gene expression at the transcriptional level, compared to the controls.
Discussion & Conclusions: In general, ATM, POLM, and TP53 genes showed a higher increased expression level in the case group, compared to the controls, which might indicate the activation of noted genes after H. pylori infection. This may subsequently activate the error-prone DNA repair and non-homologous recombination pathways. |
| first_indexed | 2024-12-14T03:44:41Z |
| format | Article |
| id | doaj.art-f9a4b2ee4c1d4b0485c2b0d43e91242c |
| institution | Directory Open Access Journal |
| issn | 1563-4728 2588-3135 |
| language | fas |
| last_indexed | 2024-12-14T03:44:41Z |
| publishDate | 2021-03-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj.art-f9a4b2ee4c1d4b0485c2b0d43e91242c2022-12-21T23:18:24ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352021-03-012918795Evaluation of Gene Expression Alterations of ATM, TP53, and POLM in Gastric Biopsy Specimens of Patients with Gastritis and its Association with Helicobacter Pylori InfectionArmin Ghameshloo0Ali Rashidi-nezhad1Masoud Alebouyeh2Abas Shakouri3 Dept of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Maternal, Fetal and Neonatal Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran Pediatric Infections Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran Genetic Ward, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran Introduction: Gastritis is one of the most common diseases affecting the stomach. Helicobacter pylori infection could lead to DNA damage in gastric epithelial cells, followed by error-prone DNA repair pathways that increase the accumulation of damage at the site of DNA double-stranded breaks, exacerbate genome instability, and facilitate the emergence of gastric cancer. This study aimed to examine the expression level of ATM, POLM, and TP53 genes involving in the DNA Damage Response and the cell cycle arrest in the gastric precancerous stage. Materials & Methods: Among 180 gastric biopsy specimens, 30 samples taken from patients with moderate chronic gastritis infected by H. Pylori were regarded as the case group, and 30 other samples taken from non-infected patients with mild chronic gastritis were regarded as control. Following that, RNA extraction and cDNA synthesis was performed. Afterward, the expression levels of ATM, POLM, and TP53 genes were evaluated by the Real-Time PCR method. Ethics code: 98-02-27-43392 Findings: The ATM, POLM, and TP53 genes in the cases showed a 4.07, 3.35, and 5.13 increase in the gene expression at the transcriptional level, compared to the controls. Discussion & Conclusions: In general, ATM, POLM, and TP53 genes showed a higher increased expression level in the case group, compared to the controls, which might indicate the activation of noted genes after H. pylori infection. This may subsequently activate the error-prone DNA repair and non-homologous recombination pathways.http://sjimu.medilam.ac.ir/article-1-6713-en.htmlatmpolmtp53 genesdna double stranded breaksgastritish. pylori |
| spellingShingle | Armin Ghameshloo Ali Rashidi-nezhad Masoud Alebouyeh Abas Shakouri Evaluation of Gene Expression Alterations of ATM, TP53, and POLM in Gastric Biopsy Specimens of Patients with Gastritis and its Association with Helicobacter Pylori Infection Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām atm polm tp53 genes dna double stranded breaks gastritis h. pylori |
| title | Evaluation of Gene Expression Alterations of ATM, TP53, and
POLM in Gastric Biopsy Specimens of Patients with
Gastritis and its Association with Helicobacter
Pylori Infection |
| title_full | Evaluation of Gene Expression Alterations of ATM, TP53, and
POLM in Gastric Biopsy Specimens of Patients with
Gastritis and its Association with Helicobacter
Pylori Infection |
| title_fullStr | Evaluation of Gene Expression Alterations of ATM, TP53, and
POLM in Gastric Biopsy Specimens of Patients with
Gastritis and its Association with Helicobacter
Pylori Infection |
| title_full_unstemmed | Evaluation of Gene Expression Alterations of ATM, TP53, and
POLM in Gastric Biopsy Specimens of Patients with
Gastritis and its Association with Helicobacter
Pylori Infection |
| title_short | Evaluation of Gene Expression Alterations of ATM, TP53, and
POLM in Gastric Biopsy Specimens of Patients with
Gastritis and its Association with Helicobacter
Pylori Infection |
| title_sort | evaluation of gene expression alterations of atm tp53 and polm in gastric biopsy specimens of patients with gastritis and its association with helicobacter pylori infection |
| topic | atm polm tp53 genes dna double stranded breaks gastritis h. pylori |
| url | http://sjimu.medilam.ac.ir/article-1-6713-en.html |
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