Adequate enrichment of extracellular vesicles in laboratory medicine
Abstract Extracellular vesicles (EVs) and their clinical applications have been recognized as one of the most fast‐growing and promising fields. Numerous groups including us have expressed their concerns on the idea of isolating “pure” EVs for different purposes, as there are no specific markers to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-07-01
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| Series: | Interdisciplinary Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/INMD.20220003 |
| _version_ | 1797769005972324352 |
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| author | Limin Zhang Wencui Ma Xinyi Gan Wanyu Chen Jiahui Guo Yizhi Cui Tong Wang |
| author_facet | Limin Zhang Wencui Ma Xinyi Gan Wanyu Chen Jiahui Guo Yizhi Cui Tong Wang |
| author_sort | Limin Zhang |
| collection | DOAJ |
| description | Abstract Extracellular vesicles (EVs) and their clinical applications have been recognized as one of the most fast‐growing and promising fields. Numerous groups including us have expressed their concerns on the idea of isolating “pure” EVs for different purposes, as there are no specific markers to date that can examine the exact EV purity. Typically, major instrument need and EV yielding loss are primary compensations of getting EVs with higher purity. As such, especially in biomarker‐based clinical tests, ceaseless efforts for obtaining more pure EV are worth of discussion. We here propose alternative philosophy to tone down the importance of purity, but to emphasize the significance in clinical laboratory medicine to measure EVs obtained by well‐controlled isolation methods. As a result, EVs are enriched to the adequate degree to meet certain clinical applications such as disease diagnosis and/or prognosis. In this review, we will justify the adequate enrichment rationale with advances of EV definitions, markers and contaminants, EV isolation methods, as well as the conclusions acquired from clinical research using less pure but ease‐to‐operate EV isolation methods. |
| first_indexed | 2024-03-12T21:02:35Z |
| format | Article |
| id | doaj.art-f9a97ca6f70445ac8723791deaae234c |
| institution | Directory Open Access Journal |
| issn | 2832-6245 |
| language | English |
| last_indexed | 2024-03-12T21:02:35Z |
| publishDate | 2023-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Interdisciplinary Medicine |
| spelling | doaj.art-f9a97ca6f70445ac8723791deaae234c2023-07-31T04:41:32ZengWiley-VCHInterdisciplinary Medicine2832-62452023-07-0113n/an/a10.1002/INMD.20220003Adequate enrichment of extracellular vesicles in laboratory medicineLimin Zhang0Wencui Ma1Xinyi Gan2Wanyu Chen3Jiahui Guo4Yizhi Cui5Tong Wang6MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health Engineering The First Affiliated Hospital Jinan University Guangzhou ChinaMOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health Engineering The First Affiliated Hospital Jinan University Guangzhou ChinaMOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health Engineering The First Affiliated Hospital Jinan University Guangzhou ChinaMOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health Engineering The First Affiliated Hospital Jinan University Guangzhou ChinaMOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health Engineering The First Affiliated Hospital Jinan University Guangzhou ChinaMOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health Engineering The First Affiliated Hospital Jinan University Guangzhou ChinaMOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health Engineering The First Affiliated Hospital Jinan University Guangzhou ChinaAbstract Extracellular vesicles (EVs) and their clinical applications have been recognized as one of the most fast‐growing and promising fields. Numerous groups including us have expressed their concerns on the idea of isolating “pure” EVs for different purposes, as there are no specific markers to date that can examine the exact EV purity. Typically, major instrument need and EV yielding loss are primary compensations of getting EVs with higher purity. As such, especially in biomarker‐based clinical tests, ceaseless efforts for obtaining more pure EV are worth of discussion. We here propose alternative philosophy to tone down the importance of purity, but to emphasize the significance in clinical laboratory medicine to measure EVs obtained by well‐controlled isolation methods. As a result, EVs are enriched to the adequate degree to meet certain clinical applications such as disease diagnosis and/or prognosis. In this review, we will justify the adequate enrichment rationale with advances of EV definitions, markers and contaminants, EV isolation methods, as well as the conclusions acquired from clinical research using less pure but ease‐to‐operate EV isolation methods.https://doi.org/10.1002/INMD.20220003adequate enrichmentclinical applicationextracellular vesicleslaboratory medicinepurity |
| spellingShingle | Limin Zhang Wencui Ma Xinyi Gan Wanyu Chen Jiahui Guo Yizhi Cui Tong Wang Adequate enrichment of extracellular vesicles in laboratory medicine Interdisciplinary Medicine adequate enrichment clinical application extracellular vesicles laboratory medicine purity |
| title | Adequate enrichment of extracellular vesicles in laboratory medicine |
| title_full | Adequate enrichment of extracellular vesicles in laboratory medicine |
| title_fullStr | Adequate enrichment of extracellular vesicles in laboratory medicine |
| title_full_unstemmed | Adequate enrichment of extracellular vesicles in laboratory medicine |
| title_short | Adequate enrichment of extracellular vesicles in laboratory medicine |
| title_sort | adequate enrichment of extracellular vesicles in laboratory medicine |
| topic | adequate enrichment clinical application extracellular vesicles laboratory medicine purity |
| url | https://doi.org/10.1002/INMD.20220003 |
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