Valorization of Polyethylene Terephthalate to Muconic Acid by Engineering <i>Pseudomonas Putida</i>
Plastic waste is rapidly accumulating in the environment and becoming a huge global challenge. Many studies have highlighted the role of microbial metabolic engineering for the valorization of polyethylene terephthalate (PET) waste. In this study, we proposed a new conceptual scheme for upcycling of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/10997 |
| _version_ | 1797479286303621120 |
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| author | Pan Liu Yi Zheng Yingbo Yuan Tong Zhang Qingbin Li Quanfeng Liang Tianyuan Su Qingsheng Qi |
| author_facet | Pan Liu Yi Zheng Yingbo Yuan Tong Zhang Qingbin Li Quanfeng Liang Tianyuan Su Qingsheng Qi |
| author_sort | Pan Liu |
| collection | DOAJ |
| description | Plastic waste is rapidly accumulating in the environment and becoming a huge global challenge. Many studies have highlighted the role of microbial metabolic engineering for the valorization of polyethylene terephthalate (PET) waste. In this study, we proposed a new conceptual scheme for upcycling of PET. We constructed a multifunctional <i>Pseudomonas putida</i> KT2440 to simultaneously secrete PET hydrolase LCC, a leaf-branch compost cutinase, and synthesize muconic acid (MA) using the PET hydrolysate. The final product MA and extracellular LCC can be separated from the supernatant of the culture by ultrafiltration, and the latter was used for the next round of PET hydrolysis. A total of 0.50 g MA was produced from 1 g PET in each cycle of the whole biological processes, reaching 68% of the theoretical conversion. This new conceptual scheme for the valorization of PET waste should have advantages over existing PET upcycling schemes and provides new ideas for the utilization of other macromolecular resources that are difficult to decompose, such as lignin. |
| first_indexed | 2024-03-09T21:43:40Z |
| format | Article |
| id | doaj.art-f9a98750813a493d91e1411cabbc03fd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:43:40Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f9a98750813a493d91e1411cabbc03fd2023-11-23T20:25:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123191099710.3390/ijms231910997Valorization of Polyethylene Terephthalate to Muconic Acid by Engineering <i>Pseudomonas Putida</i>Pan Liu0Yi Zheng1Yingbo Yuan2Tong Zhang3Qingbin Li4Quanfeng Liang5Tianyuan Su6Qingsheng Qi7State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaPlastic waste is rapidly accumulating in the environment and becoming a huge global challenge. Many studies have highlighted the role of microbial metabolic engineering for the valorization of polyethylene terephthalate (PET) waste. In this study, we proposed a new conceptual scheme for upcycling of PET. We constructed a multifunctional <i>Pseudomonas putida</i> KT2440 to simultaneously secrete PET hydrolase LCC, a leaf-branch compost cutinase, and synthesize muconic acid (MA) using the PET hydrolysate. The final product MA and extracellular LCC can be separated from the supernatant of the culture by ultrafiltration, and the latter was used for the next round of PET hydrolysis. A total of 0.50 g MA was produced from 1 g PET in each cycle of the whole biological processes, reaching 68% of the theoretical conversion. This new conceptual scheme for the valorization of PET waste should have advantages over existing PET upcycling schemes and provides new ideas for the utilization of other macromolecular resources that are difficult to decompose, such as lignin.https://www.mdpi.com/1422-0067/23/19/10997metabolic engineeringLCCpolyethylene terephthalateenzymatic hydrolysisbioconversion |
| spellingShingle | Pan Liu Yi Zheng Yingbo Yuan Tong Zhang Qingbin Li Quanfeng Liang Tianyuan Su Qingsheng Qi Valorization of Polyethylene Terephthalate to Muconic Acid by Engineering <i>Pseudomonas Putida</i> International Journal of Molecular Sciences metabolic engineering LCC polyethylene terephthalate enzymatic hydrolysis bioconversion |
| title | Valorization of Polyethylene Terephthalate to Muconic Acid by Engineering <i>Pseudomonas Putida</i> |
| title_full | Valorization of Polyethylene Terephthalate to Muconic Acid by Engineering <i>Pseudomonas Putida</i> |
| title_fullStr | Valorization of Polyethylene Terephthalate to Muconic Acid by Engineering <i>Pseudomonas Putida</i> |
| title_full_unstemmed | Valorization of Polyethylene Terephthalate to Muconic Acid by Engineering <i>Pseudomonas Putida</i> |
| title_short | Valorization of Polyethylene Terephthalate to Muconic Acid by Engineering <i>Pseudomonas Putida</i> |
| title_sort | valorization of polyethylene terephthalate to muconic acid by engineering i pseudomonas putida i |
| topic | metabolic engineering LCC polyethylene terephthalate enzymatic hydrolysis bioconversion |
| url | https://www.mdpi.com/1422-0067/23/19/10997 |
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