Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBPα
C/EBPα is a critical transcriptional regulator of adipogenesis. How C/EBPα transcription is itself regulated is poorly understood, however, and remains a key question that needs to be addressed for a complete understanding of adipogenic development. Here, we identify a lncRNA, ADINR (adipogenic diff...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-11-01
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| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671115002696 |
| _version_ | 1818317612612845568 |
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| author | Tengfei Xiao Lihui Liu Hongling Li Yu Sun Huaxia Luo Tangping Li Shihua Wang Stephen Dalton Robert Chunhua Zhao Runsheng Chen |
| author_facet | Tengfei Xiao Lihui Liu Hongling Li Yu Sun Huaxia Luo Tangping Li Shihua Wang Stephen Dalton Robert Chunhua Zhao Runsheng Chen |
| author_sort | Tengfei Xiao |
| collection | DOAJ |
| description | C/EBPα is a critical transcriptional regulator of adipogenesis. How C/EBPα transcription is itself regulated is poorly understood, however, and remains a key question that needs to be addressed for a complete understanding of adipogenic development. Here, we identify a lncRNA, ADINR (adipogenic differentiation induced noncoding RNA), transcribed from a position ∼450 bp upstream of the C/EBPα gene, that orchestrates C/EBPα transcription in vivo. Depletion of ADINR leads to a severe adipogenic defect that is rescued by overexpression of C/EBPα. Moreover, we reveal that ADINR RNA specifically binds to PA1 and recruits MLL3/4 histone methyl-transferase complexes so as to increase H3K4me3 and decrease H3K27me3 histone modification in the C/EBPα locus during adipogenesis. These results show that ADINR plays important roles in regulating the differentiation of human mesenchymal stem cells into adipocytes by modulating C/EBPα in cis. |
| first_indexed | 2024-12-13T09:40:06Z |
| format | Article |
| id | doaj.art-f9abc259158a482ca468e6d90cb42e7b |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-13T09:40:06Z |
| publishDate | 2015-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-f9abc259158a482ca468e6d90cb42e7b2022-12-21T23:52:14ZengElsevierStem Cell Reports2213-67112015-11-015585686510.1016/j.stemcr.2015.09.007Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBPαTengfei Xiao0Lihui Liu1Hongling Li2Yu Sun3Huaxia Luo4Tangping Li5Shihua Wang6Stephen Dalton7Robert Chunhua Zhao8Runsheng Chen9Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaCenter of Excellence in Tissue Engineering, Institute of Basic Medical Sciences, School of Basic Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, ChinaKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaCenter of Excellence in Tissue Engineering, Institute of Basic Medical Sciences, School of Basic Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, ChinaCenter of Excellence in Tissue Engineering, Institute of Basic Medical Sciences, School of Basic Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, ChinaPaul D. Coverdell Center for Biomedical and Health Sciences, Department of Biochemistry and Molecular Biology, The University of Georgia, 500 D.W. Brooks Drive, Athens, GA 30602, USACenter of Excellence in Tissue Engineering, Institute of Basic Medical Sciences, School of Basic Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, ChinaKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaC/EBPα is a critical transcriptional regulator of adipogenesis. How C/EBPα transcription is itself regulated is poorly understood, however, and remains a key question that needs to be addressed for a complete understanding of adipogenic development. Here, we identify a lncRNA, ADINR (adipogenic differentiation induced noncoding RNA), transcribed from a position ∼450 bp upstream of the C/EBPα gene, that orchestrates C/EBPα transcription in vivo. Depletion of ADINR leads to a severe adipogenic defect that is rescued by overexpression of C/EBPα. Moreover, we reveal that ADINR RNA specifically binds to PA1 and recruits MLL3/4 histone methyl-transferase complexes so as to increase H3K4me3 and decrease H3K27me3 histone modification in the C/EBPα locus during adipogenesis. These results show that ADINR plays important roles in regulating the differentiation of human mesenchymal stem cells into adipocytes by modulating C/EBPα in cis.http://www.sciencedirect.com/science/article/pii/S2213671115002696 |
| spellingShingle | Tengfei Xiao Lihui Liu Hongling Li Yu Sun Huaxia Luo Tangping Li Shihua Wang Stephen Dalton Robert Chunhua Zhao Runsheng Chen Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBPα Stem Cell Reports |
| title | Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBPα |
| title_full | Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBPα |
| title_fullStr | Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBPα |
| title_full_unstemmed | Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBPα |
| title_short | Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBPα |
| title_sort | long noncoding rna adinr regulates adipogenesis by transcriptionally activating c ebpα |
| url | http://www.sciencedirect.com/science/article/pii/S2213671115002696 |
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