Differentiation in the protein synthesis-dependency of persistent synaptic plasticity in mossy fiber and associational/commissural CA3 synapses in vivo
Long-term potentiation (LTP) and long-term depression (LTD) are two mechanisms involved in the long-term storage of information in hippocampal synapses. In the hippocampal CA1 region, the late phases of LTP and LTD are protein-synthesis dependent. In the dentate gyrus, late-LTP but not LTD requires...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-03-01
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| Series: | Frontiers in Integrative Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnint.2013.00010/full |
| _version_ | 1828779591304478720 |
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| author | Hardy eHagena Denise eManahan-Vaughan |
| author_facet | Hardy eHagena Denise eManahan-Vaughan |
| author_sort | Hardy eHagena |
| collection | DOAJ |
| description | Long-term potentiation (LTP) and long-term depression (LTD) are two mechanisms involved in the long-term storage of information in hippocampal synapses. In the hippocampal CA1 region, the late phases of LTP and LTD are protein-synthesis dependent. In the dentate gyrus, late-LTP but not LTD requires protein synthesis. The protein synthesis-dependency of persistent plasticity at CA3 synapses has not yet been characterized.Here, the roles of protein transcription and translation at mossy fiber (mf) and associational/commissural (AC)- synapses were studied in freely behaving rats. In control animals, low-frequency stimulation (LFS) evoked robust LTD (>24h), whereas high-frequency simulation (HFS) elicited robust LTP (>24h) at both mf-CA3 and AC-CA3 synapses. Translation inhibitors prevented early and late phases of LTP and LTD at mf–CA3 synapses. In contrast, at AC–CA3 synapses, translation inhibitors prevented intermediate/late-LTP and late-LTD only. Transcription effects were also synapse-specific: whereas transcription inhibitors inhibited late-LTP and late-LTD (>3h) at mf–CA3 synapses, at AC–CA3 synapses, protein transcription affected early-LTP and late-LTD. These results show that the AC-CA3 and mf-CA3 synapses display different properties in terms of their protein synthesis dependency, suggesting different roles in the processing of short- and long term synaptic plasticity. |
| first_indexed | 2024-12-11T17:04:28Z |
| format | Article |
| id | doaj.art-f9ad31a1461f435980c8700e2d58e25b |
| institution | Directory Open Access Journal |
| issn | 1662-5145 |
| language | English |
| last_indexed | 2024-12-11T17:04:28Z |
| publishDate | 2013-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Integrative Neuroscience |
| spelling | doaj.art-f9ad31a1461f435980c8700e2d58e25b2022-12-22T00:57:44ZengFrontiers Media S.A.Frontiers in Integrative Neuroscience1662-51452013-03-01710.3389/fnint.2013.0001041202Differentiation in the protein synthesis-dependency of persistent synaptic plasticity in mossy fiber and associational/commissural CA3 synapses in vivoHardy eHagena0Denise eManahan-Vaughan1Ruhr University BochumRuhr University BochumLong-term potentiation (LTP) and long-term depression (LTD) are two mechanisms involved in the long-term storage of information in hippocampal synapses. In the hippocampal CA1 region, the late phases of LTP and LTD are protein-synthesis dependent. In the dentate gyrus, late-LTP but not LTD requires protein synthesis. The protein synthesis-dependency of persistent plasticity at CA3 synapses has not yet been characterized.Here, the roles of protein transcription and translation at mossy fiber (mf) and associational/commissural (AC)- synapses were studied in freely behaving rats. In control animals, low-frequency stimulation (LFS) evoked robust LTD (>24h), whereas high-frequency simulation (HFS) elicited robust LTP (>24h) at both mf-CA3 and AC-CA3 synapses. Translation inhibitors prevented early and late phases of LTP and LTD at mf–CA3 synapses. In contrast, at AC–CA3 synapses, translation inhibitors prevented intermediate/late-LTP and late-LTD only. Transcription effects were also synapse-specific: whereas transcription inhibitors inhibited late-LTP and late-LTD (>3h) at mf–CA3 synapses, at AC–CA3 synapses, protein transcription affected early-LTP and late-LTD. These results show that the AC-CA3 and mf-CA3 synapses display different properties in terms of their protein synthesis dependency, suggesting different roles in the processing of short- and long term synaptic plasticity.http://journal.frontiersin.org/Journal/10.3389/fnint.2013.00010/fullCA3transcriptionmossy fibersassociational-commissural fibersprotein-synthesis |
| spellingShingle | Hardy eHagena Denise eManahan-Vaughan Differentiation in the protein synthesis-dependency of persistent synaptic plasticity in mossy fiber and associational/commissural CA3 synapses in vivo Frontiers in Integrative Neuroscience CA3 transcription mossy fibers associational-commissural fibers protein-synthesis |
| title | Differentiation in the protein synthesis-dependency of persistent synaptic plasticity in mossy fiber and associational/commissural CA3 synapses in vivo |
| title_full | Differentiation in the protein synthesis-dependency of persistent synaptic plasticity in mossy fiber and associational/commissural CA3 synapses in vivo |
| title_fullStr | Differentiation in the protein synthesis-dependency of persistent synaptic plasticity in mossy fiber and associational/commissural CA3 synapses in vivo |
| title_full_unstemmed | Differentiation in the protein synthesis-dependency of persistent synaptic plasticity in mossy fiber and associational/commissural CA3 synapses in vivo |
| title_short | Differentiation in the protein synthesis-dependency of persistent synaptic plasticity in mossy fiber and associational/commissural CA3 synapses in vivo |
| title_sort | differentiation in the protein synthesis dependency of persistent synaptic plasticity in mossy fiber and associational commissural ca3 synapses in vivo |
| topic | CA3 transcription mossy fibers associational-commissural fibers protein-synthesis |
| url | http://journal.frontiersin.org/Journal/10.3389/fnint.2013.00010/full |
| work_keys_str_mv | AT hardyehagena differentiationintheproteinsynthesisdependencyofpersistentsynapticplasticityinmossyfiberandassociationalcommissuralca3synapsesinvivo AT deniseemanahanvaughan differentiationintheproteinsynthesisdependencyofpersistentsynapticplasticityinmossyfiberandassociationalcommissuralca3synapsesinvivo |