Disposable microfluidic micromixers for effective capture of Cryptosporidium parvum oocysts from water samples
Abstract Background Protecting drinking water supplies from pathogens such as Cryptosporidium parvum is a major concern for water utilities worldwide. The sensitivity and specificity of current detection methods are largely determined by the effectiveness of the concentration and separation methods...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-03-01
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| Series: | Journal of Biological Engineering |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13036-018-0095-6 |
| _version_ | 1818544037280350208 |
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| author | L. Diéguez M. Winter S. Molan P. Monis B. King B. Thierry |
| author_facet | L. Diéguez M. Winter S. Molan P. Monis B. King B. Thierry |
| author_sort | L. Diéguez |
| collection | DOAJ |
| description | Abstract Background Protecting drinking water supplies from pathogens such as Cryptosporidium parvum is a major concern for water utilities worldwide. The sensitivity and specificity of current detection methods are largely determined by the effectiveness of the concentration and separation methods used. The purpose of this study is to develop micromixers able to specifically isolate and concentrate Cryptosporidium, while allowing in situ analysis. Results In this study, disposable microfluidic micromixers were fabricated to effectively isolate Cryptosporidium parvum oocysts from water samples, while allowing direct observation and enabling quantification of oocysts captured in the device using high quality immunofluorescence microscopy. In parallel, quantitative analysis of the capture yield was carried out by analyzing the waste from the microfluidics outlet with an Imaging Flow Cytometer. At the optimal flow rate, capture efficiencies up to 96% were achieved in spiked samples. Conclusions Scaled microfluidic isolation and detection of Cryptosporidium parvum will provide a faster and more efficient detection method for Cryptosporidium compared to other available laboratory-scale technologies. |
| first_indexed | 2024-12-11T22:43:16Z |
| format | Article |
| id | doaj.art-f9b9ec82a9f24641b0dcf6c5e5b00716 |
| institution | Directory Open Access Journal |
| issn | 1754-1611 |
| language | English |
| last_indexed | 2024-12-11T22:43:16Z |
| publishDate | 2018-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj.art-f9b9ec82a9f24641b0dcf6c5e5b007162022-12-22T00:47:43ZengBMCJournal of Biological Engineering1754-16112018-03-011211810.1186/s13036-018-0095-6Disposable microfluidic micromixers for effective capture of Cryptosporidium parvum oocysts from water samplesL. Diéguez0M. Winter1S. Molan2P. Monis3B. King4B. Thierry5Future Industries Institute and ARC Centre of Excellence in Convergent Bio and Nano Science and Technology, University of South Australia, Mawson Lakes CampusFuture Industries Institute and ARC Centre of Excellence in Convergent Bio and Nano Science and Technology, University of South Australia, Mawson Lakes CampusFuture Industries Institute and ARC Centre of Excellence in Convergent Bio and Nano Science and Technology, University of South Australia, Mawson Lakes CampusFuture Industries Institute and ARC Centre of Excellence in Convergent Bio and Nano Science and Technology, University of South Australia, Mawson Lakes CampusSouth Australian Water CorporationFuture Industries Institute and ARC Centre of Excellence in Convergent Bio and Nano Science and Technology, University of South Australia, Mawson Lakes CampusAbstract Background Protecting drinking water supplies from pathogens such as Cryptosporidium parvum is a major concern for water utilities worldwide. The sensitivity and specificity of current detection methods are largely determined by the effectiveness of the concentration and separation methods used. The purpose of this study is to develop micromixers able to specifically isolate and concentrate Cryptosporidium, while allowing in situ analysis. Results In this study, disposable microfluidic micromixers were fabricated to effectively isolate Cryptosporidium parvum oocysts from water samples, while allowing direct observation and enabling quantification of oocysts captured in the device using high quality immunofluorescence microscopy. In parallel, quantitative analysis of the capture yield was carried out by analyzing the waste from the microfluidics outlet with an Imaging Flow Cytometer. At the optimal flow rate, capture efficiencies up to 96% were achieved in spiked samples. Conclusions Scaled microfluidic isolation and detection of Cryptosporidium parvum will provide a faster and more efficient detection method for Cryptosporidium compared to other available laboratory-scale technologies.http://link.springer.com/article/10.1186/s13036-018-0095-6Cryptosporidium parvum oocystsDisposable microfluidic micromixersImaging flow cytometryWater qualityImmunocytochemistryFluorescence microscopy |
| spellingShingle | L. Diéguez M. Winter S. Molan P. Monis B. King B. Thierry Disposable microfluidic micromixers for effective capture of Cryptosporidium parvum oocysts from water samples Journal of Biological Engineering Cryptosporidium parvum oocysts Disposable microfluidic micromixers Imaging flow cytometry Water quality Immunocytochemistry Fluorescence microscopy |
| title | Disposable microfluidic micromixers for effective capture of Cryptosporidium parvum oocysts from water samples |
| title_full | Disposable microfluidic micromixers for effective capture of Cryptosporidium parvum oocysts from water samples |
| title_fullStr | Disposable microfluidic micromixers for effective capture of Cryptosporidium parvum oocysts from water samples |
| title_full_unstemmed | Disposable microfluidic micromixers for effective capture of Cryptosporidium parvum oocysts from water samples |
| title_short | Disposable microfluidic micromixers for effective capture of Cryptosporidium parvum oocysts from water samples |
| title_sort | disposable microfluidic micromixers for effective capture of cryptosporidium parvum oocysts from water samples |
| topic | Cryptosporidium parvum oocysts Disposable microfluidic micromixers Imaging flow cytometry Water quality Immunocytochemistry Fluorescence microscopy |
| url | http://link.springer.com/article/10.1186/s13036-018-0095-6 |
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