Functional interplay between Mediator and RSC chromatin remodeling complex controls nucleosome-depleted region maintenance at promoters
Summary: Chromatin organization is crucial for transcriptional regulation in eukaryotes. Mediator is an essential and conserved co-activator thought to act in concert with chromatin regulators. However, it remains largely unknown how their functions are coordinated. Here, we provide evidence in the...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112472300476X |
| _version_ | 1797833661229301760 |
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| author | Kévin M. André Nathalie Giordanengo Aiach Veronica Martinez-Fernandez Leo Zeitler Adriana Alberti Arach Goldar Michel Werner Cyril Denby Wilkes Julie Soutourina |
| author_facet | Kévin M. André Nathalie Giordanengo Aiach Veronica Martinez-Fernandez Leo Zeitler Adriana Alberti Arach Goldar Michel Werner Cyril Denby Wilkes Julie Soutourina |
| author_sort | Kévin M. André |
| collection | DOAJ |
| description | Summary: Chromatin organization is crucial for transcriptional regulation in eukaryotes. Mediator is an essential and conserved co-activator thought to act in concert with chromatin regulators. However, it remains largely unknown how their functions are coordinated. Here, we provide evidence in the yeast Saccharomyces cerevisiae that Mediator establishes physical contact with RSC (Remodels the Structure of Chromatin), a conserved and essential chromatin remodeling complex that is crucial for nucleosome-depleted region (NDR) formation. We determine the role of Mediator-RSC interaction in their chromatin binding, nucleosome occupancy, and transcription on a genomic scale. Mediator and RSC co-localize on wide NDRs of promoter regions, and specific Mediator mutations affect nucleosome eviction and TSS-associated +1 nucleosome stability. This work shows that Mediator contributes to RSC remodeling function to shape NDRs and maintain chromatin organization on promoter regions. It will help in our understanding of transcriptional regulation in the chromatin context relevant for severe diseases. |
| first_indexed | 2024-04-09T14:27:47Z |
| format | Article |
| id | doaj.art-f9bbea9526e54585825f206e6c20bd6d |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-09T14:27:47Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-f9bbea9526e54585825f206e6c20bd6d2023-05-04T04:12:22ZengElsevierCell Reports2211-12472023-05-01425112465Functional interplay between Mediator and RSC chromatin remodeling complex controls nucleosome-depleted region maintenance at promotersKévin M. André0Nathalie Giordanengo Aiach1Veronica Martinez-Fernandez2Leo Zeitler3Adriana Alberti4Arach Goldar5Michel Werner6Cyril Denby Wilkes7Julie Soutourina8Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, FranceUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, FranceUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, FranceUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, FranceUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, FranceUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, FranceUniversité Paris Cité, CNRS, Institut Jacques Monod, 75013 Paris, FranceUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, FranceUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, France; Corresponding authorSummary: Chromatin organization is crucial for transcriptional regulation in eukaryotes. Mediator is an essential and conserved co-activator thought to act in concert with chromatin regulators. However, it remains largely unknown how their functions are coordinated. Here, we provide evidence in the yeast Saccharomyces cerevisiae that Mediator establishes physical contact with RSC (Remodels the Structure of Chromatin), a conserved and essential chromatin remodeling complex that is crucial for nucleosome-depleted region (NDR) formation. We determine the role of Mediator-RSC interaction in their chromatin binding, nucleosome occupancy, and transcription on a genomic scale. Mediator and RSC co-localize on wide NDRs of promoter regions, and specific Mediator mutations affect nucleosome eviction and TSS-associated +1 nucleosome stability. This work shows that Mediator contributes to RSC remodeling function to shape NDRs and maintain chromatin organization on promoter regions. It will help in our understanding of transcriptional regulation in the chromatin context relevant for severe diseases.http://www.sciencedirect.com/science/article/pii/S221112472300476XCP: Molecular biology |
| spellingShingle | Kévin M. André Nathalie Giordanengo Aiach Veronica Martinez-Fernandez Leo Zeitler Adriana Alberti Arach Goldar Michel Werner Cyril Denby Wilkes Julie Soutourina Functional interplay between Mediator and RSC chromatin remodeling complex controls nucleosome-depleted region maintenance at promoters Cell Reports CP: Molecular biology |
| title | Functional interplay between Mediator and RSC chromatin remodeling complex controls nucleosome-depleted region maintenance at promoters |
| title_full | Functional interplay between Mediator and RSC chromatin remodeling complex controls nucleosome-depleted region maintenance at promoters |
| title_fullStr | Functional interplay between Mediator and RSC chromatin remodeling complex controls nucleosome-depleted region maintenance at promoters |
| title_full_unstemmed | Functional interplay between Mediator and RSC chromatin remodeling complex controls nucleosome-depleted region maintenance at promoters |
| title_short | Functional interplay between Mediator and RSC chromatin remodeling complex controls nucleosome-depleted region maintenance at promoters |
| title_sort | functional interplay between mediator and rsc chromatin remodeling complex controls nucleosome depleted region maintenance at promoters |
| topic | CP: Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S221112472300476X |
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