Dual Optical Nanosensor Based on Ormosil Nanoparticles for Monitoring O<sub>2</sub> and pH
Monitoring O<sub>2</sub> and pH has excellent potential in different sensing applications, especially in biological and clinical applications. This report presents a protocol for synthesizing an optical dual nanosensor for those two parameters. The organically modified silica (ormosil) n...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/11/1011 |
| Summary: | Monitoring O<sub>2</sub> and pH has excellent potential in different sensing applications, especially in biological and clinical applications. This report presents a protocol for synthesizing an optical dual nanosensor for those two parameters. The organically modified silica (ormosil) nanoparticles were prepared based on phenytrimethoxysilane in an aqueous solution using an acid-base one-pot strategy. Ormosil was selected as a lipophilic matrix for loading fluorescent O<sub>2</sub>-sensitive dye platinum(II)-tetrakis-(pentafluorophenyl) porphyrin (Pt-TPFPP), which was quenched in the presence of O<sub>2</sub> gas and exhibited a considerable detection proficiency within a percentage range of (0–100%) O<sub>2</sub>. Commercially available drug ingredient salicylamide was labeled on the surface of the nanoparticles using a coupling agent (3-glycidoxypropyl) trimethoxysilane (GPTMS). For measuring pH, salicylamide acted for the first time as a pH-sensitive probe based on a turn-on process with increasing pH. The nanosensor displayed a significant pH detection efficiency in the range of (pH = 6–10). Salicylamide turn-on fluorescence was attributed to the excited state intramolecular transfer (ESIPT) process followed by the inter charge transfer (ICT). The presented dual nanosensor opens new opportunities as a promising candidate material for industrial systems and medical applications. |
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| ISSN: | 2079-6374 |