Sarcoidosis and tuberculosis cytokine profiles: indistinguishable in bronchoalveolar lavage but different in blood.
The clinical, radiological and pathological similarities between sarcoidosis and tuberculosis can make disease differentiation challenging. A complicating factor is that some cases of sarcoidosis may be initiated by mycobacteria. We hypothesised that immunological profiling might provide insight int...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3398021?pdf=render |
| _version_ | 1828172677628559360 |
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| author | Muhunthan Thillai Christian Eberhardt Alex M Lewin Lee Potiphar Suzie Hingley-Wilson Saranya Sridhar Jonathan Macintyre Onn Min Kon Melissa Wickremasinghe Athol Wells Mark E Weeks Donald Mitchell Ajit Lalvani |
| author_facet | Muhunthan Thillai Christian Eberhardt Alex M Lewin Lee Potiphar Suzie Hingley-Wilson Saranya Sridhar Jonathan Macintyre Onn Min Kon Melissa Wickremasinghe Athol Wells Mark E Weeks Donald Mitchell Ajit Lalvani |
| author_sort | Muhunthan Thillai |
| collection | DOAJ |
| description | The clinical, radiological and pathological similarities between sarcoidosis and tuberculosis can make disease differentiation challenging. A complicating factor is that some cases of sarcoidosis may be initiated by mycobacteria. We hypothesised that immunological profiling might provide insight into a possible relationship between the diseases or allow us to distinguish between them.We analysed bronchoalveolar lavage (BAL) fluid in sarcoidosis (n = 18), tuberculosis (n = 12) and healthy volunteers (n = 16). We further investigated serum samples in the same groups; sarcoidosis (n = 40), tuberculosis (n = 15) and healthy volunteers (n = 40). A cross-sectional analysis of multiple cytokine profiles was performed and data used to discriminate between samples.We found that BAL profiles were indistinguishable between both diseases and significantly different from healthy volunteers. In sera, tuberculosis patients had significantly lower levels of the Th2 cytokine interleukin-4 (IL-4) than those with sarcoidosis (p = 0.004). Additional serum differences allowed us to create a linear regression model for disease differentiation (within-sample accuracy 91%, cross-validation accuracy 73%).These data warrant replication in independent cohorts to further develop and validate a serum cytokine signature that may be able to distinguish sarcoidosis from tuberculosis. Systemic Th2 cytokine differences between sarcoidosis and tuberculosis may also underly different disease outcomes to similar respiratory stimuli. |
| first_indexed | 2024-04-12T03:43:46Z |
| format | Article |
| id | doaj.art-f9bf74ef955145b3b509f7b2e87e6685 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T03:43:46Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f9bf74ef955145b3b509f7b2e87e66852022-12-22T03:49:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e3808310.1371/journal.pone.0038083Sarcoidosis and tuberculosis cytokine profiles: indistinguishable in bronchoalveolar lavage but different in blood.Muhunthan ThillaiChristian EberhardtAlex M LewinLee PotipharSuzie Hingley-WilsonSaranya SridharJonathan MacintyreOnn Min KonMelissa WickremasingheAthol WellsMark E WeeksDonald MitchellAjit LalvaniThe clinical, radiological and pathological similarities between sarcoidosis and tuberculosis can make disease differentiation challenging. A complicating factor is that some cases of sarcoidosis may be initiated by mycobacteria. We hypothesised that immunological profiling might provide insight into a possible relationship between the diseases or allow us to distinguish between them.We analysed bronchoalveolar lavage (BAL) fluid in sarcoidosis (n = 18), tuberculosis (n = 12) and healthy volunteers (n = 16). We further investigated serum samples in the same groups; sarcoidosis (n = 40), tuberculosis (n = 15) and healthy volunteers (n = 40). A cross-sectional analysis of multiple cytokine profiles was performed and data used to discriminate between samples.We found that BAL profiles were indistinguishable between both diseases and significantly different from healthy volunteers. In sera, tuberculosis patients had significantly lower levels of the Th2 cytokine interleukin-4 (IL-4) than those with sarcoidosis (p = 0.004). Additional serum differences allowed us to create a linear regression model for disease differentiation (within-sample accuracy 91%, cross-validation accuracy 73%).These data warrant replication in independent cohorts to further develop and validate a serum cytokine signature that may be able to distinguish sarcoidosis from tuberculosis. Systemic Th2 cytokine differences between sarcoidosis and tuberculosis may also underly different disease outcomes to similar respiratory stimuli.http://europepmc.org/articles/PMC3398021?pdf=render |
| spellingShingle | Muhunthan Thillai Christian Eberhardt Alex M Lewin Lee Potiphar Suzie Hingley-Wilson Saranya Sridhar Jonathan Macintyre Onn Min Kon Melissa Wickremasinghe Athol Wells Mark E Weeks Donald Mitchell Ajit Lalvani Sarcoidosis and tuberculosis cytokine profiles: indistinguishable in bronchoalveolar lavage but different in blood. PLoS ONE |
| title | Sarcoidosis and tuberculosis cytokine profiles: indistinguishable in bronchoalveolar lavage but different in blood. |
| title_full | Sarcoidosis and tuberculosis cytokine profiles: indistinguishable in bronchoalveolar lavage but different in blood. |
| title_fullStr | Sarcoidosis and tuberculosis cytokine profiles: indistinguishable in bronchoalveolar lavage but different in blood. |
| title_full_unstemmed | Sarcoidosis and tuberculosis cytokine profiles: indistinguishable in bronchoalveolar lavage but different in blood. |
| title_short | Sarcoidosis and tuberculosis cytokine profiles: indistinguishable in bronchoalveolar lavage but different in blood. |
| title_sort | sarcoidosis and tuberculosis cytokine profiles indistinguishable in bronchoalveolar lavage but different in blood |
| url | http://europepmc.org/articles/PMC3398021?pdf=render |
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