Analysis of fertility potential in men with severe azoospermia and oligospermia of various etiology
The study objective is to evaluate the significance of the Y chromosome microdeletions for prediction of spermatozoa retrieval during testicle biopsy in men with severe azoospermia and oligozoospermia.Materials and methods. In total, 109 men aged 21 to 56 years (mean age 32.7 ± 0.2 years) with infer...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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ABV-press
2018-11-01
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| Series: | Андрология и генитальная хирургия |
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| Online Access: | https://agx.abvpress.ru/jour/article/view/310 |
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| author | T. A. Yamandi L. V. Akulenko N. Yu. Safina I. I. Vityazeva S. V. Bogolubov Ya. I. Melnik E. V. Kasatonova A. V. Polyakov M. V. Andreeva V. B. Chernykh |
| author_facet | T. A. Yamandi L. V. Akulenko N. Yu. Safina I. I. Vityazeva S. V. Bogolubov Ya. I. Melnik E. V. Kasatonova A. V. Polyakov M. V. Andreeva V. B. Chernykh |
| author_sort | T. A. Yamandi |
| collection | DOAJ |
| description | The study objective is to evaluate the significance of the Y chromosome microdeletions for prediction of spermatozoa retrieval during testicle biopsy in men with severe azoospermia and oligozoospermia.Materials and methods. In total, 109 men aged 21 to 56 years (mean age 32.7 ± 0.2 years) with infertility in marriage were examined. Cytogenetic, special andrological, spermiological, and molecular genetic examinations were performed to evaluate non-genetic and genetic causes of infertility.Results. Normal karyotype and absence of AZF deletions were found in 75 men, presence of deletions – in 34. The frequencies of pathozoospermia forms were comparable in these groups. Spermatozoa were retrieved during biopsy in 47 (62.7 %) patients without Y chromosome microdeletions and only in 7 (20.6 %) patients with Y chromosome microdeletions. The men with AZF deletions were divided into 2 subgroups: men with complete AZF region deletions (n = 25) and men with partial AZF deletions (n = 9). Among men with complete deletions, azoospermia was diagnosed in 25 (100 %), spermatozoa were retrieved during biopsy in 2 (8 %); among men with partial deletions, azoospermia was diagnosed in 7 (77.8 %), severe oligozoospermia in 2 (22.2 %), spermatozoa were retrieved during biopsy in 5 (56 %). Then the patients were divided according to another criterion: 54 patients from whom spermatozoa were retrieved during biopsy and 55 men with negative results. Among patients with successful result of biopsy, Y chromosome microdeletions were identified in 7 (13 %); among patient with negative biopsy result – in 27 (49 %) (р < 0.01).Conclusion. Success rate of spermatozoa retrieval during testicle biopsy is significantly higher in men without AZF deletions (р < 0.01) than in men with deletions. Molecular genetic examination of Y chromosome microdeletions is recommended for men with azoospermia and severe oligozoospermia because it allows diagnosing of cause male infertility and predicting. |
| first_indexed | 2024-04-10T03:53:20Z |
| format | Article |
| id | doaj.art-f9bfc85646064463937d29cbf44854e4 |
| institution | Directory Open Access Journal |
| issn | 2070-9781 |
| language | Russian |
| last_indexed | 2024-04-10T03:53:20Z |
| publishDate | 2018-11-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Андрология и генитальная хирургия |
| spelling | doaj.art-f9bfc85646064463937d29cbf44854e42023-03-13T07:15:09ZrusABV-pressАндрология и генитальная хирургия2070-97812018-11-01193606910.17650/2070-9781-2018-19-3-60-69282Analysis of fertility potential in men with severe azoospermia and oligospermia of various etiologyT. A. Yamandi0L. V. Akulenko1N. Yu. Safina2I. I. Vityazeva3S. V. Bogolubov4Ya. I. Melnik5E. V. Kasatonova6A. V. Polyakov7M. V. Andreeva8V. B. Chernykh9A.I. Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of RussiaA.I. Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of RussiaA.I. Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of RussiaNational Medical Research Center of Endocrinology, Ministry of Health of RussiaNational Medical Research Center of Endocrinology, Ministry of Health of RussiaN.А. Lopatkin Scientific Research Institute of Urology and Interventional Radiology – branch of the National Medical Research Radiologiсal Center, Ministry of Health of RussiaN.А. Lopatkin Scientific Research Institute of Urology and Interventional Radiology – branch of the National Medical Research Radiologiсal Center, Ministry of Health of RussiaResearch Centre for Medical GeneticsResearch Centre for Medical GeneticsResearch Centre for Medical Genetics; N.I. Pirogov Russian National Research Medical University, Ministry of Health of RussiaThe study objective is to evaluate the significance of the Y chromosome microdeletions for prediction of spermatozoa retrieval during testicle biopsy in men with severe azoospermia and oligozoospermia.Materials and methods. In total, 109 men aged 21 to 56 years (mean age 32.7 ± 0.2 years) with infertility in marriage were examined. Cytogenetic, special andrological, spermiological, and molecular genetic examinations were performed to evaluate non-genetic and genetic causes of infertility.Results. Normal karyotype and absence of AZF deletions were found in 75 men, presence of deletions – in 34. The frequencies of pathozoospermia forms were comparable in these groups. Spermatozoa were retrieved during biopsy in 47 (62.7 %) patients without Y chromosome microdeletions and only in 7 (20.6 %) patients with Y chromosome microdeletions. The men with AZF deletions were divided into 2 subgroups: men with complete AZF region deletions (n = 25) and men with partial AZF deletions (n = 9). Among men with complete deletions, azoospermia was diagnosed in 25 (100 %), spermatozoa were retrieved during biopsy in 2 (8 %); among men with partial deletions, azoospermia was diagnosed in 7 (77.8 %), severe oligozoospermia in 2 (22.2 %), spermatozoa were retrieved during biopsy in 5 (56 %). Then the patients were divided according to another criterion: 54 patients from whom spermatozoa were retrieved during biopsy and 55 men with negative results. Among patients with successful result of biopsy, Y chromosome microdeletions were identified in 7 (13 %); among patient with negative biopsy result – in 27 (49 %) (р < 0.01).Conclusion. Success rate of spermatozoa retrieval during testicle biopsy is significantly higher in men without AZF deletions (р < 0.01) than in men with deletions. Molecular genetic examination of Y chromosome microdeletions is recommended for men with azoospermia and severe oligozoospermia because it allows diagnosing of cause male infertility and predicting.https://agx.abvpress.ru/jour/article/view/310testicular biopsyy chromosomeazf locusdeletionsmale infertilityhuman reproductionchromosomal anomaliesin vitro fertilizationivf/icsi |
| spellingShingle | T. A. Yamandi L. V. Akulenko N. Yu. Safina I. I. Vityazeva S. V. Bogolubov Ya. I. Melnik E. V. Kasatonova A. V. Polyakov M. V. Andreeva V. B. Chernykh Analysis of fertility potential in men with severe azoospermia and oligospermia of various etiology Андрология и генитальная хирургия testicular biopsy y chromosome azf locus deletions male infertility human reproduction chromosomal anomalies in vitro fertilization ivf/icsi |
| title | Analysis of fertility potential in men with severe azoospermia and oligospermia of various etiology |
| title_full | Analysis of fertility potential in men with severe azoospermia and oligospermia of various etiology |
| title_fullStr | Analysis of fertility potential in men with severe azoospermia and oligospermia of various etiology |
| title_full_unstemmed | Analysis of fertility potential in men with severe azoospermia and oligospermia of various etiology |
| title_short | Analysis of fertility potential in men with severe azoospermia and oligospermia of various etiology |
| title_sort | analysis of fertility potential in men with severe azoospermia and oligospermia of various etiology |
| topic | testicular biopsy y chromosome azf locus deletions male infertility human reproduction chromosomal anomalies in vitro fertilization ivf/icsi |
| url | https://agx.abvpress.ru/jour/article/view/310 |
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