Report a Novel Mutation in Human Prostacyclin Receptor Gene in patient affected with Migraine
Objective: The human prostacyclin receptor gene (PTGIR) encodes the human prostacyclin (PGI2) receptor. PTGIR is a part of vasodilator system during the migraine attacks and probably has an important role in the mechanism of this disease. Materials and Methods: We used direct PCR and sequencing to d...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2017-06-01
|
| Series: | Iranian Journal of Psychiatry |
| Subjects: | |
| Online Access: | https://ijps.tums.ac.ir/index.php/ijps/article/view/843 |
| _version_ | 1818833596502245376 |
|---|---|
| author | Majid Kheirollahi Mohammad Reza Pourreza Fariborz Khorvash Mohammad Kazemi Gilda Amini |
| author_facet | Majid Kheirollahi Mohammad Reza Pourreza Fariborz Khorvash Mohammad Kazemi Gilda Amini |
| author_sort | Majid Kheirollahi |
| collection | DOAJ |
| description | Objective: The human prostacyclin receptor gene (PTGIR) encodes the human prostacyclin (PGI2) receptor. PTGIR is a part of vasodilator system during the migraine attacks and probably has an important role in the mechanism of this disease. Materials and Methods: We used direct PCR and sequencing to determine the any variants in PTGIR gene. A blood sample was collected from the patients and genomic DNA was extracted. Polymerase chain reaction was carried out on extracted DNA. The PCR products were then sequenced using a cycle sequencing kit, on an automated DNA sequencing machine. Results: In reviewing of familial and clinopathological of these two patients, both patients have migraines with visual aura and their mothers also are suffering from migraines. Their parents had been married strangers. Direct sequencing analysis of exon 2 of the PTGIR gene showing the presence of two mutations in two patients. These mutations were heterozygote that made the following changes; g.1626T>A, c.754T>A, cDNA.867T>A, and p.S252T for the first mutation and c.753C>T, cDNA866C>T, g.1625C>T, p.C251C for the second mutation. The first mutation alters the amino acid and is a novel mutation. The second change is a conservative mutation that have already been reported. Conclusion: The prediction results predicted the variant would negatively affect the protein’s function and seems to be disease causing. Although functional analysis is required to confirm the association between the variant and the disease. |
| first_indexed | 2024-12-19T02:21:26Z |
| format | Article |
| id | doaj.art-f9c3c8ea774b47c890dded8cf152268c |
| institution | Directory Open Access Journal |
| issn | 1735-4587 2008-2215 |
| language | English |
| last_indexed | 2024-12-19T02:21:26Z |
| publishDate | 2017-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Psychiatry |
| spelling | doaj.art-f9c3c8ea774b47c890dded8cf152268c2022-12-21T20:40:10ZengTehran University of Medical SciencesIranian Journal of Psychiatry1735-45872008-22152017-06-01123598Report a Novel Mutation in Human Prostacyclin Receptor Gene in patient affected with MigraineMajid Kheirollahi0Mohammad Reza Pourreza1Fariborz Khorvash2Mohammad Kazemi3Gilda Amini4Isfahan University of Medical Sciences, Isfahan, IranIsfahan University of Medical Sciences, Isfahan, IranIsfahan University of Medical Sciences, Isfahan, IranIsfahan University of Medical Sciences, Isfahan, IranIsfahan University of Medical Sciences, Isfahan, IranObjective: The human prostacyclin receptor gene (PTGIR) encodes the human prostacyclin (PGI2) receptor. PTGIR is a part of vasodilator system during the migraine attacks and probably has an important role in the mechanism of this disease. Materials and Methods: We used direct PCR and sequencing to determine the any variants in PTGIR gene. A blood sample was collected from the patients and genomic DNA was extracted. Polymerase chain reaction was carried out on extracted DNA. The PCR products were then sequenced using a cycle sequencing kit, on an automated DNA sequencing machine. Results: In reviewing of familial and clinopathological of these two patients, both patients have migraines with visual aura and their mothers also are suffering from migraines. Their parents had been married strangers. Direct sequencing analysis of exon 2 of the PTGIR gene showing the presence of two mutations in two patients. These mutations were heterozygote that made the following changes; g.1626T>A, c.754T>A, cDNA.867T>A, and p.S252T for the first mutation and c.753C>T, cDNA866C>T, g.1625C>T, p.C251C for the second mutation. The first mutation alters the amino acid and is a novel mutation. The second change is a conservative mutation that have already been reported. Conclusion: The prediction results predicted the variant would negatively affect the protein’s function and seems to be disease causing. Although functional analysis is required to confirm the association between the variant and the disease.https://ijps.tums.ac.ir/index.php/ijps/article/view/843MigraineMutationProstacyclin Receptor Gene |
| spellingShingle | Majid Kheirollahi Mohammad Reza Pourreza Fariborz Khorvash Mohammad Kazemi Gilda Amini Report a Novel Mutation in Human Prostacyclin Receptor Gene in patient affected with Migraine Iranian Journal of Psychiatry Migraine Mutation Prostacyclin Receptor Gene |
| title | Report a Novel Mutation in Human Prostacyclin Receptor Gene in patient affected with Migraine |
| title_full | Report a Novel Mutation in Human Prostacyclin Receptor Gene in patient affected with Migraine |
| title_fullStr | Report a Novel Mutation in Human Prostacyclin Receptor Gene in patient affected with Migraine |
| title_full_unstemmed | Report a Novel Mutation in Human Prostacyclin Receptor Gene in patient affected with Migraine |
| title_short | Report a Novel Mutation in Human Prostacyclin Receptor Gene in patient affected with Migraine |
| title_sort | report a novel mutation in human prostacyclin receptor gene in patient affected with migraine |
| topic | Migraine Mutation Prostacyclin Receptor Gene |
| url | https://ijps.tums.ac.ir/index.php/ijps/article/view/843 |
| work_keys_str_mv | AT majidkheirollahi reportanovelmutationinhumanprostacyclinreceptorgeneinpatientaffectedwithmigraine AT mohammadrezapourreza reportanovelmutationinhumanprostacyclinreceptorgeneinpatientaffectedwithmigraine AT fariborzkhorvash reportanovelmutationinhumanprostacyclinreceptorgeneinpatientaffectedwithmigraine AT mohammadkazemi reportanovelmutationinhumanprostacyclinreceptorgeneinpatientaffectedwithmigraine AT gildaamini reportanovelmutationinhumanprostacyclinreceptorgeneinpatientaffectedwithmigraine |