Detection of Barley yellow mosaic virus from Soil Using Nested PCR
Barley yellow mosaic virus (BaYMV), which is transmitted by the root-inhabiting protist Polymyxa graminis, causes a soil-borne disease. In this study, we detected BaYMV from soil using two-step nested polymerase chain reaction (PCR). Specific primers based on a coat protein region of BaYMV segment R...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2017-03-01
|
| Series: | Research in Plant Disease |
| Subjects: | |
| Online Access: | http://www.online-rpd.org/journal/view.html?doi=10.5423/RPD.2017.23.1.65 |
| _version_ | 1828407009625505792 |
|---|---|
| author | Joong-Hwan Lee Chang-Gi Son Joong-Bae Kwon Hyo-Hun Nam Yeong-Tae Kim Bong-Choon Lee Dong-Bum Shin |
| author_facet | Joong-Hwan Lee Chang-Gi Son Joong-Bae Kwon Hyo-Hun Nam Yeong-Tae Kim Bong-Choon Lee Dong-Bum Shin |
| author_sort | Joong-Hwan Lee |
| collection | DOAJ |
| description | Barley yellow mosaic virus (BaYMV), which is transmitted by the root-inhabiting protist Polymyxa graminis, causes a soil-borne disease. In this study, we detected BaYMV from soil using two-step nested polymerase chain reaction (PCR). Specific primers based on a coat protein region of BaYMV segment RNA1 were used in the first round of amplification. Based on the sequenced amplicon, an inner primer was designed for the second round of amplification. A PCR product of 372 bp exhibited 98%–100% nucleotide sequence identity with the coat protein region of BaYMV segment RNA1. In this study, we propose an easy method for the detection of BaYMV from soil, may considerably assist in accurate fungus-transmitted virus diagnosis and subsequent disease forecasting. This is the first report on the detection of BaYMV from soil. |
| first_indexed | 2024-12-10T11:19:14Z |
| format | Article |
| id | doaj.art-f9c68613fc9141dd943209a5468c3aa8 |
| institution | Directory Open Access Journal |
| issn | 1598-2262 |
| language | English |
| last_indexed | 2024-12-10T11:19:14Z |
| publishDate | 2017-03-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj.art-f9c68613fc9141dd943209a5468c3aa82022-12-22T01:51:03ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622017-03-01231656810.5423/RPD.2017.23.1.65RPD.2017.23.1.65Detection of Barley yellow mosaic virus from Soil Using Nested PCRJoong-Hwan Lee0Chang-Gi Son1Joong-Bae Kwon2Hyo-Hun Nam3Yeong-Tae Kim4Bong-Choon Lee5Dong-Bum Shin6Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services, Andong 36614, KoreaInstitute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services, Andong 36614, KoreaInstitute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services, Andong 36614, KoreaInstitute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services, Andong 36614, KoreaInstitute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services, Andong 36614, KoreaCrop Foundation Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaCrop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration, Suwon 16616, KoreaBarley yellow mosaic virus (BaYMV), which is transmitted by the root-inhabiting protist Polymyxa graminis, causes a soil-borne disease. In this study, we detected BaYMV from soil using two-step nested polymerase chain reaction (PCR). Specific primers based on a coat protein region of BaYMV segment RNA1 were used in the first round of amplification. Based on the sequenced amplicon, an inner primer was designed for the second round of amplification. A PCR product of 372 bp exhibited 98%–100% nucleotide sequence identity with the coat protein region of BaYMV segment RNA1. In this study, we propose an easy method for the detection of BaYMV from soil, may considerably assist in accurate fungus-transmitted virus diagnosis and subsequent disease forecasting. This is the first report on the detection of BaYMV from soil.http://www.online-rpd.org/journal/view.html?doi=10.5423/RPD.2017.23.1.65Barley yellow mosaic virusNested PCRPolymyxa graminis |
| spellingShingle | Joong-Hwan Lee Chang-Gi Son Joong-Bae Kwon Hyo-Hun Nam Yeong-Tae Kim Bong-Choon Lee Dong-Bum Shin Detection of Barley yellow mosaic virus from Soil Using Nested PCR Research in Plant Disease Barley yellow mosaic virus Nested PCR Polymyxa graminis |
| title | Detection of Barley yellow mosaic virus from Soil Using Nested PCR |
| title_full | Detection of Barley yellow mosaic virus from Soil Using Nested PCR |
| title_fullStr | Detection of Barley yellow mosaic virus from Soil Using Nested PCR |
| title_full_unstemmed | Detection of Barley yellow mosaic virus from Soil Using Nested PCR |
| title_short | Detection of Barley yellow mosaic virus from Soil Using Nested PCR |
| title_sort | detection of barley yellow mosaic virus from soil using nested pcr |
| topic | Barley yellow mosaic virus Nested PCR Polymyxa graminis |
| url | http://www.online-rpd.org/journal/view.html?doi=10.5423/RPD.2017.23.1.65 |
| work_keys_str_mv | AT joonghwanlee detectionofbarleyyellowmosaicvirusfromsoilusingnestedpcr AT changgison detectionofbarleyyellowmosaicvirusfromsoilusingnestedpcr AT joongbaekwon detectionofbarleyyellowmosaicvirusfromsoilusingnestedpcr AT hyohunnam detectionofbarleyyellowmosaicvirusfromsoilusingnestedpcr AT yeongtaekim detectionofbarleyyellowmosaicvirusfromsoilusingnestedpcr AT bongchoonlee detectionofbarleyyellowmosaicvirusfromsoilusingnestedpcr AT dongbumshin detectionofbarleyyellowmosaicvirusfromsoilusingnestedpcr |