Chemical Profiling and Biological Screening of Some River Nile Derived-Microorganisms
AimsChemical and biological studies of the River Nile derived-microorganisms are limited. Hence, this work was carried out to screen the River Nile habitat. Identification of the isolated organisms, chemical profiling of their ethyl acetate extracts as well as screening of their antimicrobial, antil...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00787/full |
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| author | Momen M. Lotfy Hossam M. Hassan Hossam M. Hassan Rabab Mohammed Mona Hetta Ahmed O. El-Gendy Mostafa E. Rateb Mostafa E. Rateb Mohamed A. Zaki Noha M. Gamaleldin Noha M. Gamaleldin |
| author_facet | Momen M. Lotfy Hossam M. Hassan Hossam M. Hassan Rabab Mohammed Mona Hetta Ahmed O. El-Gendy Mostafa E. Rateb Mostafa E. Rateb Mohamed A. Zaki Noha M. Gamaleldin Noha M. Gamaleldin |
| author_sort | Momen M. Lotfy |
| collection | DOAJ |
| description | AimsChemical and biological studies of the River Nile derived-microorganisms are limited. Hence, this work was carried out to screen the River Nile habitat. Identification of the isolated organisms, chemical profiling of their ethyl acetate extracts as well as screening of their antimicrobial, antileishmanial, antitrypanosomal, and antimalarial activities were investigated.MethodsIdentification of the microbial isolates were carried out using bacterial 16S rRNA and fungal 18S rRNA gene sequencing. Chemical profiling of the EtOAc extracts using LC-HRESIMS spectroscopy was carried out. The in vitro antimicrobial screening using the modified version of the CLSI method, antileishmanial and antitrypanosomal activities were screened using Leishmania donovani promastigote assay, L. donovani axenic amastigote assay, Trypanosoma brucei trypamastigotes assay and THP1 toxicity assay. The in vitro antimalarial activities against D6 (chloroquine sensitive) and W2 (chloroquine-resistant) strains of Plasmodium falciparum were evaluated.ResultsSeven isolated microorganisms were identified as Streptomyces indiaensis, Bacillus safensis, B. anthracis, Bacillus sp., and Aspergillus awamori. Chemical investigation of different extracts showed several bioactive compounds, identified as; nigragillin, 5-caboxybenzofuran and dyramide B from A. awamori and actinopolysporin B from S. indiaensis. On the other hand many nitrogenous compounds with high molecular weights showed no hits that may correspond to new long chain and/or cyclic peptides. The EtOAc extract of B. safensis fermentation broth showed the highest activity against P. falciparum D6 and P. falciparum W2 (IC50 = 25.94 and 27.28 μg/mL, respectively), while two isolates S. indiaensis and Bacillus sp. RN-011 extracts showed the highest antitrypanosomal activity (IC50 = 0.8 and 0.96 μg/mL).ConclusionThe River Nile could be a new source for production of promising bioactive leading compound where antimicrobial and antiparasitic activities may be correlated. |
| first_indexed | 2024-12-14T12:28:27Z |
| format | Article |
| id | doaj.art-f9c6a10b138f47e1a4b51b9ffc3d837b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T12:28:27Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f9c6a10b138f47e1a4b51b9ffc3d837b2022-12-21T23:01:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-04-011010.3389/fmicb.2019.00787435978Chemical Profiling and Biological Screening of Some River Nile Derived-MicroorganismsMomen M. Lotfy0Hossam M. Hassan1Hossam M. Hassan2Rabab Mohammed3Mona Hetta4Ahmed O. El-Gendy5Mostafa E. Rateb6Mostafa E. Rateb7Mohamed A. Zaki8Noha M. Gamaleldin9Noha M. Gamaleldin10Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Fayoum University, Fayoum, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Beni- Suef University, Beni-Suef, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, EgyptSchool of Computing, Engineering and Physical Sciences, University of the West of Scotland, Paisley, United KingdomDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, EgyptDepartment of Microbiology, Faculty of Pharmacy, The British University in Egypt (BUE), El-Sherouk, EgyptThe Center for Drug Research and Development (CDRD), The British University in Egypt (BUE), El-Sherouk, EgyptAimsChemical and biological studies of the River Nile derived-microorganisms are limited. Hence, this work was carried out to screen the River Nile habitat. Identification of the isolated organisms, chemical profiling of their ethyl acetate extracts as well as screening of their antimicrobial, antileishmanial, antitrypanosomal, and antimalarial activities were investigated.MethodsIdentification of the microbial isolates were carried out using bacterial 16S rRNA and fungal 18S rRNA gene sequencing. Chemical profiling of the EtOAc extracts using LC-HRESIMS spectroscopy was carried out. The in vitro antimicrobial screening using the modified version of the CLSI method, antileishmanial and antitrypanosomal activities were screened using Leishmania donovani promastigote assay, L. donovani axenic amastigote assay, Trypanosoma brucei trypamastigotes assay and THP1 toxicity assay. The in vitro antimalarial activities against D6 (chloroquine sensitive) and W2 (chloroquine-resistant) strains of Plasmodium falciparum were evaluated.ResultsSeven isolated microorganisms were identified as Streptomyces indiaensis, Bacillus safensis, B. anthracis, Bacillus sp., and Aspergillus awamori. Chemical investigation of different extracts showed several bioactive compounds, identified as; nigragillin, 5-caboxybenzofuran and dyramide B from A. awamori and actinopolysporin B from S. indiaensis. On the other hand many nitrogenous compounds with high molecular weights showed no hits that may correspond to new long chain and/or cyclic peptides. The EtOAc extract of B. safensis fermentation broth showed the highest activity against P. falciparum D6 and P. falciparum W2 (IC50 = 25.94 and 27.28 μg/mL, respectively), while two isolates S. indiaensis and Bacillus sp. RN-011 extracts showed the highest antitrypanosomal activity (IC50 = 0.8 and 0.96 μg/mL).ConclusionThe River Nile could be a new source for production of promising bioactive leading compound where antimicrobial and antiparasitic activities may be correlated.https://www.frontiersin.org/article/10.3389/fmicb.2019.00787/fullchemical profilingantimicrobialantileishmanialantitrypanosomalantimalarial |
| spellingShingle | Momen M. Lotfy Hossam M. Hassan Hossam M. Hassan Rabab Mohammed Mona Hetta Ahmed O. El-Gendy Mostafa E. Rateb Mostafa E. Rateb Mohamed A. Zaki Noha M. Gamaleldin Noha M. Gamaleldin Chemical Profiling and Biological Screening of Some River Nile Derived-Microorganisms Frontiers in Microbiology chemical profiling antimicrobial antileishmanial antitrypanosomal antimalarial |
| title | Chemical Profiling and Biological Screening of Some River Nile Derived-Microorganisms |
| title_full | Chemical Profiling and Biological Screening of Some River Nile Derived-Microorganisms |
| title_fullStr | Chemical Profiling and Biological Screening of Some River Nile Derived-Microorganisms |
| title_full_unstemmed | Chemical Profiling and Biological Screening of Some River Nile Derived-Microorganisms |
| title_short | Chemical Profiling and Biological Screening of Some River Nile Derived-Microorganisms |
| title_sort | chemical profiling and biological screening of some river nile derived microorganisms |
| topic | chemical profiling antimicrobial antileishmanial antitrypanosomal antimalarial |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00787/full |
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