Comparative Whey Proteome Profiling of Donkey Milk With Human and Cow Milk
Donkey milk (DM), similar to human milk (HM) in chemical composition, has been suggested as the best potential hypoallergenic replacement diet for babies suffering from Cow milk (CM) protein allergy. In order to better understand DM protein, many studies based on proteomic have been performed. In th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.911454/full |
| _version_ | 1811326495449153536 |
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| author | Xinhao Zhang Xinhao Zhang Guimiao Jiang Chuanliang Ji Zhaobin Fan Shihao Ge Haijing Li Yantao Wang Xin Lv Fuwei Zhao |
| author_facet | Xinhao Zhang Xinhao Zhang Guimiao Jiang Chuanliang Ji Zhaobin Fan Shihao Ge Haijing Li Yantao Wang Xin Lv Fuwei Zhao |
| author_sort | Xinhao Zhang |
| collection | DOAJ |
| description | Donkey milk (DM), similar to human milk (HM) in chemical composition, has been suggested as the best potential hypoallergenic replacement diet for babies suffering from Cow milk (CM) protein allergy. In order to better understand DM protein, many studies based on proteomic have been performed. In this study, the label-free quantitative proteomic approach was conducted to quantitatively identify the differentially expressed whey proteins (DEPs) in DM vs. HM group and DM vs. CM group. In total, 241 and 365 DEPs were found in these two groups, respectively. Bioinformatics analysis of DEPs showed that the majority of DEPs participated in the lipoprotein metabolic process, regulation of cytokine production, chemical homeostasis, and catabolic process. The Kyoto Encyclopedia of Gene and Genomes (KEGG) pathways analysis found that these DEPs mainly participated in an antigen processing, complement, and coagulation cascades. These results may provide valuable information in the composition of milk whey proteins in DM, HM, and CM, especially for low abundant components, and expand our knowledge of different biological functions between DM and HM or CM. |
| first_indexed | 2024-04-13T14:50:17Z |
| format | Article |
| id | doaj.art-f9c734584d064beea3595bd95271d431 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-13T14:50:17Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-f9c734584d064beea3595bd95271d4312022-12-22T02:42:37ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-06-01910.3389/fnut.2022.911454911454Comparative Whey Proteome Profiling of Donkey Milk With Human and Cow MilkXinhao Zhang0Xinhao Zhang1Guimiao Jiang2Chuanliang Ji3Zhaobin Fan4Shihao Ge5Haijing Li6Yantao Wang7Xin Lv8Fuwei Zhao9Department of Animal Pharmacy, College of Pharmacy, Heze University, Heze, ChinaNational Engineering Research Center for Gelatin-Based TCM, Dong-E E-Jiao Co., Ltd., Liaocheng, ChinaNational Engineering Research Center for Gelatin-Based TCM, Dong-E E-Jiao Co., Ltd., Liaocheng, ChinaNational Engineering Research Center for Gelatin-Based TCM, Dong-E E-Jiao Co., Ltd., Liaocheng, ChinaDepartment of Animal Pharmacy, College of Pharmacy, Heze University, Heze, ChinaDepartment of Animal Pharmacy, College of Pharmacy, Heze University, Heze, ChinaNational Engineering Research Center for Gelatin-Based TCM, Dong-E E-Jiao Co., Ltd., Liaocheng, ChinaNational Engineering Research Center for Gelatin-Based TCM, Dong-E E-Jiao Co., Ltd., Liaocheng, ChinaNational Engineering Research Center for Gelatin-Based TCM, Dong-E E-Jiao Co., Ltd., Liaocheng, ChinaDepartment of Animal Pharmacy, College of Pharmacy, Heze University, Heze, ChinaDonkey milk (DM), similar to human milk (HM) in chemical composition, has been suggested as the best potential hypoallergenic replacement diet for babies suffering from Cow milk (CM) protein allergy. In order to better understand DM protein, many studies based on proteomic have been performed. In this study, the label-free quantitative proteomic approach was conducted to quantitatively identify the differentially expressed whey proteins (DEPs) in DM vs. HM group and DM vs. CM group. In total, 241 and 365 DEPs were found in these two groups, respectively. Bioinformatics analysis of DEPs showed that the majority of DEPs participated in the lipoprotein metabolic process, regulation of cytokine production, chemical homeostasis, and catabolic process. The Kyoto Encyclopedia of Gene and Genomes (KEGG) pathways analysis found that these DEPs mainly participated in an antigen processing, complement, and coagulation cascades. These results may provide valuable information in the composition of milk whey proteins in DM, HM, and CM, especially for low abundant components, and expand our knowledge of different biological functions between DM and HM or CM.https://www.frontiersin.org/articles/10.3389/fnut.2022.911454/fulldonkey milkhuman milkcow milkwheyproteome |
| spellingShingle | Xinhao Zhang Xinhao Zhang Guimiao Jiang Chuanliang Ji Zhaobin Fan Shihao Ge Haijing Li Yantao Wang Xin Lv Fuwei Zhao Comparative Whey Proteome Profiling of Donkey Milk With Human and Cow Milk Frontiers in Nutrition donkey milk human milk cow milk whey proteome |
| title | Comparative Whey Proteome Profiling of Donkey Milk With Human and Cow Milk |
| title_full | Comparative Whey Proteome Profiling of Donkey Milk With Human and Cow Milk |
| title_fullStr | Comparative Whey Proteome Profiling of Donkey Milk With Human and Cow Milk |
| title_full_unstemmed | Comparative Whey Proteome Profiling of Donkey Milk With Human and Cow Milk |
| title_short | Comparative Whey Proteome Profiling of Donkey Milk With Human and Cow Milk |
| title_sort | comparative whey proteome profiling of donkey milk with human and cow milk |
| topic | donkey milk human milk cow milk whey proteome |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.911454/full |
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