De novo sequencing of the leaf transcriptome reveals complex light-responsive regulatory networks in Camellia sinensis cv. Baijiguan
Tea plants (Camellia sinensis L.) possess high genetic diversity that is important for breeding. One cultivar, Baijiguan, exhibits a yellow leaf phenotype, reduced chlorophyll (Chl) content and aberrant chloroplast structures under high light intensity. In contrast, under low light intensity, the fl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-03-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00332/full |
| _version_ | 1818624392143306752 |
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| author | Quanjin eWu Zhidan eChen Weijiang eSun Tingting eDeng Mingjie eChen |
| author_facet | Quanjin eWu Zhidan eChen Weijiang eSun Tingting eDeng Mingjie eChen |
| author_sort | Quanjin eWu |
| collection | DOAJ |
| description | Tea plants (Camellia sinensis L.) possess high genetic diversity that is important for breeding. One cultivar, Baijiguan, exhibits a yellow leaf phenotype, reduced chlorophyll (Chl) content and aberrant chloroplast structures under high light intensity. In contrast, under low light intensity, the flush shoot from Baijiguan becomes green, the Chl content increases significantly, and the chloroplasts exhibit normal structures. To understand the underlying molecular mechanisms for these observations, we performed de novo transcriptome sequencing and digital gene expression (DGE) profiling using Illumina sequencing technology. De novo transcriptome assembly identified 88,788 unigenes, including 1652 transcription factors from 25 families. In total, 1993 and 2576 differentially expressed genes (DEGs) were identified in Baijiguan plants treated with three and six days of shade, respectively. Gene Ontology (GO) and pathway enrichment analyses indicated that the DEGs are predominantly involved in the ROS scavenging system, chloroplast development, photosynthetic pigment synthesis, secondary metabolism and circadian systems. The light-responsive gene POR (protochlorophyllide oxidoreductase) and transcription factor HY5 were identified. Quantitative real-time PCR (qRT-PCR) analysis of 20 selected DEGs confirmed the RNA-sequencing (RNA-Seq) results. Overall, these findings suggest that high light intensity inhibits the expression of photosystem II 10-kDa protein (PsbR) in Baijiguan, thus affecting PSII stability, chloroplast development and chlorophyll biosynthesis. |
| first_indexed | 2024-12-16T18:56:13Z |
| format | Article |
| id | doaj.art-f9c7c6a2b1ac4b73be35813fd91a30f8 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T18:56:13Z |
| publishDate | 2016-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f9c7c6a2b1ac4b73be35813fd91a30f82022-12-21T22:20:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-03-01710.3389/fpls.2016.00332160284De novo sequencing of the leaf transcriptome reveals complex light-responsive regulatory networks in Camellia sinensis cv. BaijiguanQuanjin eWu0Zhidan eChen1Weijiang eSun2Tingting eDeng3Mingjie eChen4Fujian Agriculture And Forestry UniversityFujian Agriculture And Forestry UniversityFujian Agriculture And Forestry UniversityFujian Agriculture And Forestry UniversityFujian Agriculture And Forestry UniversityTea plants (Camellia sinensis L.) possess high genetic diversity that is important for breeding. One cultivar, Baijiguan, exhibits a yellow leaf phenotype, reduced chlorophyll (Chl) content and aberrant chloroplast structures under high light intensity. In contrast, under low light intensity, the flush shoot from Baijiguan becomes green, the Chl content increases significantly, and the chloroplasts exhibit normal structures. To understand the underlying molecular mechanisms for these observations, we performed de novo transcriptome sequencing and digital gene expression (DGE) profiling using Illumina sequencing technology. De novo transcriptome assembly identified 88,788 unigenes, including 1652 transcription factors from 25 families. In total, 1993 and 2576 differentially expressed genes (DEGs) were identified in Baijiguan plants treated with three and six days of shade, respectively. Gene Ontology (GO) and pathway enrichment analyses indicated that the DEGs are predominantly involved in the ROS scavenging system, chloroplast development, photosynthetic pigment synthesis, secondary metabolism and circadian systems. The light-responsive gene POR (protochlorophyllide oxidoreductase) and transcription factor HY5 were identified. Quantitative real-time PCR (qRT-PCR) analysis of 20 selected DEGs confirmed the RNA-sequencing (RNA-Seq) results. Overall, these findings suggest that high light intensity inhibits the expression of photosystem II 10-kDa protein (PsbR) in Baijiguan, thus affecting PSII stability, chloroplast development and chlorophyll biosynthesis.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00332/fullCamellia sinensisCHLOROPHYLL SYNTHESISAntioxidant EnzymesChloroplast developmentDifferentially expressed genesLight-shading |
| spellingShingle | Quanjin eWu Zhidan eChen Weijiang eSun Tingting eDeng Mingjie eChen De novo sequencing of the leaf transcriptome reveals complex light-responsive regulatory networks in Camellia sinensis cv. Baijiguan Frontiers in Plant Science Camellia sinensis CHLOROPHYLL SYNTHESIS Antioxidant Enzymes Chloroplast development Differentially expressed genes Light-shading |
| title | De novo sequencing of the leaf transcriptome reveals complex light-responsive regulatory networks in Camellia sinensis cv. Baijiguan |
| title_full | De novo sequencing of the leaf transcriptome reveals complex light-responsive regulatory networks in Camellia sinensis cv. Baijiguan |
| title_fullStr | De novo sequencing of the leaf transcriptome reveals complex light-responsive regulatory networks in Camellia sinensis cv. Baijiguan |
| title_full_unstemmed | De novo sequencing of the leaf transcriptome reveals complex light-responsive regulatory networks in Camellia sinensis cv. Baijiguan |
| title_short | De novo sequencing of the leaf transcriptome reveals complex light-responsive regulatory networks in Camellia sinensis cv. Baijiguan |
| title_sort | de novo sequencing of the leaf transcriptome reveals complex light responsive regulatory networks in camellia sinensis cv baijiguan |
| topic | Camellia sinensis CHLOROPHYLL SYNTHESIS Antioxidant Enzymes Chloroplast development Differentially expressed genes Light-shading |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00332/full |
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