Seasonal Variation of Melatonin Concentration and mRNA Expression of Melatonin-Related Genes in Developing Ovarian Follicles of Mares Kept under Natural Photoperiods in the Southern Hemisphere
This study investigated the seasonal variations in mRNA expression of FSH (<i>Fshr</i>), LH (<i>Lhr</i>) receptors, melatonin (<i>Mt1</i> and <i>Mt2</i>) receptors, melatonin-synthetizing enzymes (<i>Asmt</i> and <i>Aanat</i>) a...
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MDPI AG
2023-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/6/1063 |
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| author | Lia Alencar Coelho Luciano Andrade Silva Ana Paula Reway Daniella Do Carmo Buonfiglio Jéssica Andrade-Silva Patrícia Rodrigues Lourenço Gomes José Cipolla-Neto |
| author_facet | Lia Alencar Coelho Luciano Andrade Silva Ana Paula Reway Daniella Do Carmo Buonfiglio Jéssica Andrade-Silva Patrícia Rodrigues Lourenço Gomes José Cipolla-Neto |
| author_sort | Lia Alencar Coelho |
| collection | DOAJ |
| description | This study investigated the seasonal variations in mRNA expression of FSH (<i>Fshr</i>), LH (<i>Lhr</i>) receptors, melatonin (<i>Mt1</i> and <i>Mt2</i>) receptors, melatonin-synthetizing enzymes (<i>Asmt</i> and <i>Aanat</i>) and melatonin concentration in developing follicles from mares raised in natural photoperiods. For one year, ultrasonographic follicular aspiration procedures were performed monthly, and small (<20 mm), medium (20 to 35 mm) and large (>35 mm) follicles were recovered from five mares. One day before monthly sample collections, an exploratory ultrasonography conducted to record the number and the size of all follicles larger than 15 mm. The total number of large follicles were higher during the spring/summer (8.2 ± 1.9) than during autumn/winter (3.0 ± 0.5). Compared to autumn/winter seasons, there was an increase of <i>Fshr</i> and <i>Aanat</i> mRNA expressions in small, medium and large follicles, an increase of <i>Lhr</i> and <i>Asmt</i> mRNA expressions in medium and large follicles and an increase of <i>Mt1</i> and <i>Mt2</i> mRNA expressions in small and large follicles during spring/summer. The melatonin levels in follicular fluid were also higher during the spring/summer seasons. The present data show that melatonin locally upregulates the mRNA expression of <i>Mt1</i> and <i>Mt2</i> receptors and melatonin-forming enzymes in mare developing follicles during reproductive seasons. |
| first_indexed | 2024-03-11T07:02:07Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T07:02:07Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-f9c826bd80f1459b9b0d7d46bcf47fa12023-11-17T09:11:50ZengMDPI AGAnimals2076-26152023-03-01136106310.3390/ani13061063Seasonal Variation of Melatonin Concentration and mRNA Expression of Melatonin-Related Genes in Developing Ovarian Follicles of Mares Kept under Natural Photoperiods in the Southern HemisphereLia Alencar Coelho0Luciano Andrade Silva1Ana Paula Reway2Daniella Do Carmo Buonfiglio3Jéssica Andrade-Silva4Patrícia Rodrigues Lourenço Gomes5José Cipolla-Neto6Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo, Pirassununga 13635-900, BrazilDepartment of Veterinary Medicine, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo, Pirassununga 13635-900, BrazilDepartment of Veterinary Medicine, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo, Pirassununga 13635-900, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences (ICB), University of São Paulo, São Paulo 05508-000, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences (ICB), University of São Paulo, São Paulo 05508-000, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences (ICB), University of São Paulo, São Paulo 05508-000, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences (ICB), University of São Paulo, São Paulo 05508-000, BrazilThis study investigated the seasonal variations in mRNA expression of FSH (<i>Fshr</i>), LH (<i>Lhr</i>) receptors, melatonin (<i>Mt1</i> and <i>Mt2</i>) receptors, melatonin-synthetizing enzymes (<i>Asmt</i> and <i>Aanat</i>) and melatonin concentration in developing follicles from mares raised in natural photoperiods. For one year, ultrasonographic follicular aspiration procedures were performed monthly, and small (<20 mm), medium (20 to 35 mm) and large (>35 mm) follicles were recovered from five mares. One day before monthly sample collections, an exploratory ultrasonography conducted to record the number and the size of all follicles larger than 15 mm. The total number of large follicles were higher during the spring/summer (8.2 ± 1.9) than during autumn/winter (3.0 ± 0.5). Compared to autumn/winter seasons, there was an increase of <i>Fshr</i> and <i>Aanat</i> mRNA expressions in small, medium and large follicles, an increase of <i>Lhr</i> and <i>Asmt</i> mRNA expressions in medium and large follicles and an increase of <i>Mt1</i> and <i>Mt2</i> mRNA expressions in small and large follicles during spring/summer. The melatonin levels in follicular fluid were also higher during the spring/summer seasons. The present data show that melatonin locally upregulates the mRNA expression of <i>Mt1</i> and <i>Mt2</i> receptors and melatonin-forming enzymes in mare developing follicles during reproductive seasons.https://www.mdpi.com/2076-2615/13/6/1063<i>Asmt</i><i>Aanat</i>follicular fluidgonadotropin receptorsmaresmelatonin receptors |
| spellingShingle | Lia Alencar Coelho Luciano Andrade Silva Ana Paula Reway Daniella Do Carmo Buonfiglio Jéssica Andrade-Silva Patrícia Rodrigues Lourenço Gomes José Cipolla-Neto Seasonal Variation of Melatonin Concentration and mRNA Expression of Melatonin-Related Genes in Developing Ovarian Follicles of Mares Kept under Natural Photoperiods in the Southern Hemisphere Animals <i>Asmt</i> <i>Aanat</i> follicular fluid gonadotropin receptors mares melatonin receptors |
| title | Seasonal Variation of Melatonin Concentration and mRNA Expression of Melatonin-Related Genes in Developing Ovarian Follicles of Mares Kept under Natural Photoperiods in the Southern Hemisphere |
| title_full | Seasonal Variation of Melatonin Concentration and mRNA Expression of Melatonin-Related Genes in Developing Ovarian Follicles of Mares Kept under Natural Photoperiods in the Southern Hemisphere |
| title_fullStr | Seasonal Variation of Melatonin Concentration and mRNA Expression of Melatonin-Related Genes in Developing Ovarian Follicles of Mares Kept under Natural Photoperiods in the Southern Hemisphere |
| title_full_unstemmed | Seasonal Variation of Melatonin Concentration and mRNA Expression of Melatonin-Related Genes in Developing Ovarian Follicles of Mares Kept under Natural Photoperiods in the Southern Hemisphere |
| title_short | Seasonal Variation of Melatonin Concentration and mRNA Expression of Melatonin-Related Genes in Developing Ovarian Follicles of Mares Kept under Natural Photoperiods in the Southern Hemisphere |
| title_sort | seasonal variation of melatonin concentration and mrna expression of melatonin related genes in developing ovarian follicles of mares kept under natural photoperiods in the southern hemisphere |
| topic | <i>Asmt</i> <i>Aanat</i> follicular fluid gonadotropin receptors mares melatonin receptors |
| url | https://www.mdpi.com/2076-2615/13/6/1063 |
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