Characterization of Acquired Resistance in Lesion-Mimic Transgenic Potato Expressing Bacterio-Opsin
The lesion-mimic mutants of certain plants display necrotic lesions resembling those of the hypersensitive response and activate local and systemic defense responses in the absence of pathogens. We have engineered a lesion-mimic phenotype in transgenic Russet Burbank potato plants through constituti...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1997-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.5.635 |
| _version_ | 1811264614010191872 |
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| author | Mark S. Abad Salim M. Hakimi Wojciech K. Kaniewski Caius M. T. Rommens Vladimir Shulaev Eric Lam Dilip M. Shah |
| author_facet | Mark S. Abad Salim M. Hakimi Wojciech K. Kaniewski Caius M. T. Rommens Vladimir Shulaev Eric Lam Dilip M. Shah |
| author_sort | Mark S. Abad |
| collection | DOAJ |
| description | The lesion-mimic mutants of certain plants display necrotic lesions resembling those of the hypersensitive response and activate local and systemic defense responses in the absence of pathogens. We have engineered a lesion-mimic phenotype in transgenic Russet Burbank potato plants through constitutive expression of a bacterio-opsin (bO) proton pump derived from Halobacterium halobium. Transgenic potato plants exhibiting a lesion-mimic phenotype had increased levels of salicylic acid and overexpressed several pathogenesis-related messenger RNAs, all hallmarks of systemic acquired resistance (SAR). The lesion-mimic plants also displayed enhanced resistance to the US1 isolate (A1 mating type) of a fungal pathogen, Phytophthora infestans, a causal agent of late blight disease. In contrast, little resistance was observed against the US8 isolate (A2 mating type) of this pathogen. Furthermore, a majority of the transgenic plants displaying the lesion-mimic phenotype had increased susceptibility to potato virus X. The tubers of these plants were not resistant to the bacterial pathogen Erwinia carotovora. These results indicate that expression of bO can result in the activation of defense responses in transgenic potato plants and show for the first time that bO expression can confer resistance to a pathogenic fungus. However, our results also demonstrate that like SAR, this “engineered” resistance is likely to be limited to certain pathogens and particular cultivars. |
| first_indexed | 2024-04-12T20:06:23Z |
| format | Article |
| id | doaj.art-f9ca1acc193742daa26693d56824a62f |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:06:23Z |
| publishDate | 1997-07-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-f9ca1acc193742daa26693d56824a62f2022-12-22T03:18:23ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061997-07-0110563564510.1094/MPMI.1997.10.5.635Characterization of Acquired Resistance in Lesion-Mimic Transgenic Potato Expressing Bacterio-OpsinMark S. AbadSalim M. HakimiWojciech K. KaniewskiCaius M. T. RommensVladimir ShulaevEric LamDilip M. ShahThe lesion-mimic mutants of certain plants display necrotic lesions resembling those of the hypersensitive response and activate local and systemic defense responses in the absence of pathogens. We have engineered a lesion-mimic phenotype in transgenic Russet Burbank potato plants through constitutive expression of a bacterio-opsin (bO) proton pump derived from Halobacterium halobium. Transgenic potato plants exhibiting a lesion-mimic phenotype had increased levels of salicylic acid and overexpressed several pathogenesis-related messenger RNAs, all hallmarks of systemic acquired resistance (SAR). The lesion-mimic plants also displayed enhanced resistance to the US1 isolate (A1 mating type) of a fungal pathogen, Phytophthora infestans, a causal agent of late blight disease. In contrast, little resistance was observed against the US8 isolate (A2 mating type) of this pathogen. Furthermore, a majority of the transgenic plants displaying the lesion-mimic phenotype had increased susceptibility to potato virus X. The tubers of these plants were not resistant to the bacterial pathogen Erwinia carotovora. These results indicate that expression of bO can result in the activation of defense responses in transgenic potato plants and show for the first time that bO expression can confer resistance to a pathogenic fungus. However, our results also demonstrate that like SAR, this “engineered” resistance is likely to be limited to certain pathogens and particular cultivars.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.5.635 |
| spellingShingle | Mark S. Abad Salim M. Hakimi Wojciech K. Kaniewski Caius M. T. Rommens Vladimir Shulaev Eric Lam Dilip M. Shah Characterization of Acquired Resistance in Lesion-Mimic Transgenic Potato Expressing Bacterio-Opsin Molecular Plant-Microbe Interactions |
| title | Characterization of Acquired Resistance in Lesion-Mimic Transgenic Potato Expressing Bacterio-Opsin |
| title_full | Characterization of Acquired Resistance in Lesion-Mimic Transgenic Potato Expressing Bacterio-Opsin |
| title_fullStr | Characterization of Acquired Resistance in Lesion-Mimic Transgenic Potato Expressing Bacterio-Opsin |
| title_full_unstemmed | Characterization of Acquired Resistance in Lesion-Mimic Transgenic Potato Expressing Bacterio-Opsin |
| title_short | Characterization of Acquired Resistance in Lesion-Mimic Transgenic Potato Expressing Bacterio-Opsin |
| title_sort | characterization of acquired resistance in lesion mimic transgenic potato expressing bacterio opsin |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.5.635 |
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