Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows
Objective The current research was aimed to profile the transcriptomic picture of the peripheral blood lymphocytes (PBLs) associated with immunity in Chinese Holsteins supplemented orally with coated folic acid during the periparturient period. Methods The total of 123 perinatal cows were selected f...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2020-09-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-18-0852.pdf |
| _version_ | 1818211733154562048 |
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| author | Muhammad Zahoor Khan Zhichao Zhang Lei Liu Di Wang Siyuan Mi Xueqin Liu Gang Liu Gang Guo Xizhi Li Yachun Wang Ying Yu |
| author_facet | Muhammad Zahoor Khan Zhichao Zhang Lei Liu Di Wang Siyuan Mi Xueqin Liu Gang Liu Gang Guo Xizhi Li Yachun Wang Ying Yu |
| author_sort | Muhammad Zahoor Khan |
| collection | DOAJ |
| description | Objective The current research was aimed to profile the transcriptomic picture of the peripheral blood lymphocytes (PBLs) associated with immunity in Chinese Holsteins supplemented orally with coated folic acid during the periparturient period. Methods The total of 123 perinatal cows were selected for this study and divided into three groups; group A (n = 41, 240 mg/500 kg cow/d), group B (n = 40, 120 mg/500 kg cow/d) and group C (n = 42, 0 mg/cow/d) based on the quantity of folic acid fed. Three samples of PBLs were selected from each folic acid treated group (high, low, and control) and RNA sequencing method was carried out for transcriptomic analysis. Results The analysis revealed that a higher number of genes and pathways were regulated in response to high and low folic acid supplementation compared to the controls. We reported the novel pathways tumor necrosis factor (TNF) signaling, antigen processing and presentation, Staphylococcus aureus infection and nuclear factor (NF)-kappa B signaling pathways) and the key genes (e.g. C-X-C motif chemokine ligand 10, TNF receptor superfamily member 1A, cluster difference 4, major histocompatibility complex, class II, DQ beta, NF-kappa-B inhibitor alpha, and TNF superfamily 13) having great importance in immunity and anti-inflammation in the periparturient cows in response to coated folic acid treatment. Conclusion Collectively, our study profiled first-time transcriptomic analysis of bovine lymphocytes and compared the involved cytokines, genes, and pathways between high vs control and low vs control. Our data suggest that the low folic acid supplementation (120 mg/500 kg) could be a good choice to boost appropriate immunity and anti-inflammation as well as might being applied to the health improvement of perinatal dairy cows. |
| first_indexed | 2024-12-12T05:37:11Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-12T05:37:11Z |
| publishDate | 2020-09-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-f9cb221e385849fcad780484c7f5acf32022-12-22T00:36:06ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172020-09-013391507151910.5713/ajas.18.085224263Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cowsMuhammad Zahoor Khan0Zhichao Zhang1Lei Liu2Di Wang3Siyuan Mi4Xueqin Liu5Gang Liu6Gang Guo7Xizhi Li8Yachun Wang9Ying Yu10 Key Laboratory of Animal Genetics, Breeding, and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China Key Laboratory of Animal Genetics, Breeding, and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China Key Laboratory of Animal Genetics, Breeding, and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China Key Laboratory of Animal Genetics, Breeding, and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China Key Laboratory of Animal Genetics, Breeding, and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China Key Laboratory of Animal Genetics, Breeding, and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China Hebei Shoulon Modern Agricultural Science and Technology Co. Ltd., Dingzhou 073000, China Hebei Shoulon Modern Agricultural Science and Technology Co. Ltd., Dingzhou 073000, China Beijing Sanyuan Breeding Technology Co. Ltd., Capital Agribusiness Group, Beijing 100193, China Key Laboratory of Animal Genetics, Breeding, and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China Key Laboratory of Animal Genetics, Breeding, and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaObjective The current research was aimed to profile the transcriptomic picture of the peripheral blood lymphocytes (PBLs) associated with immunity in Chinese Holsteins supplemented orally with coated folic acid during the periparturient period. Methods The total of 123 perinatal cows were selected for this study and divided into three groups; group A (n = 41, 240 mg/500 kg cow/d), group B (n = 40, 120 mg/500 kg cow/d) and group C (n = 42, 0 mg/cow/d) based on the quantity of folic acid fed. Three samples of PBLs were selected from each folic acid treated group (high, low, and control) and RNA sequencing method was carried out for transcriptomic analysis. Results The analysis revealed that a higher number of genes and pathways were regulated in response to high and low folic acid supplementation compared to the controls. We reported the novel pathways tumor necrosis factor (TNF) signaling, antigen processing and presentation, Staphylococcus aureus infection and nuclear factor (NF)-kappa B signaling pathways) and the key genes (e.g. C-X-C motif chemokine ligand 10, TNF receptor superfamily member 1A, cluster difference 4, major histocompatibility complex, class II, DQ beta, NF-kappa-B inhibitor alpha, and TNF superfamily 13) having great importance in immunity and anti-inflammation in the periparturient cows in response to coated folic acid treatment. Conclusion Collectively, our study profiled first-time transcriptomic analysis of bovine lymphocytes and compared the involved cytokines, genes, and pathways between high vs control and low vs control. Our data suggest that the low folic acid supplementation (120 mg/500 kg) could be a good choice to boost appropriate immunity and anti-inflammation as well as might being applied to the health improvement of perinatal dairy cows.http://www.ajas.info/upload/pdf/ajas-18-0852.pdffolic acidperiparturient dairy cowtranscriptomeanti-inflammationimmunity |
| spellingShingle | Muhammad Zahoor Khan Zhichao Zhang Lei Liu Di Wang Siyuan Mi Xueqin Liu Gang Liu Gang Guo Xizhi Li Yachun Wang Ying Yu Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows Asian-Australasian Journal of Animal Sciences folic acid periparturient dairy cow transcriptome anti-inflammation immunity |
| title | Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows |
| title_full | Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows |
| title_fullStr | Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows |
| title_full_unstemmed | Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows |
| title_short | Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows |
| title_sort | folic acid supplementation regulates key immunity associated genes and pathways during the periparturient period in dairy cows |
| topic | folic acid periparturient dairy cow transcriptome anti-inflammation immunity |
| url | http://www.ajas.info/upload/pdf/ajas-18-0852.pdf |
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