Comparative Effects of Coenzyme Q10 or <i>n</i>-3 Polyunsaturated Fatty Acid Supplementation on Retinal Angiogenesis in a Rat Model of Oxygen-Induced Retinopathy
Neonatal intermittent hypoxia (IH) or apnea afflicts 70% to 90% of all preterm infants <28 weeks gestation, and is associated with severe retinopathy of prematurity (ROP). We tested the hypotheses that coenzyme Q10 (CoQ10) or omega-3 polyunsaturated fatty acids (<i>n</i>-3 PUFAs)...
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MDPI AG
2018-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/7/11/160 |
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| author | Kay D. Beharry Charles L. Cai Faisal Siddiqui Sara Chowdhury Christina D’Agrosa Gloria B. Valencia Jacob V. Aranda |
| author_facet | Kay D. Beharry Charles L. Cai Faisal Siddiqui Sara Chowdhury Christina D’Agrosa Gloria B. Valencia Jacob V. Aranda |
| author_sort | Kay D. Beharry |
| collection | DOAJ |
| description | Neonatal intermittent hypoxia (IH) or apnea afflicts 70% to 90% of all preterm infants <28 weeks gestation, and is associated with severe retinopathy of prematurity (ROP). We tested the hypotheses that coenzyme Q10 (CoQ10) or omega-3 polyunsaturated fatty acids (<i>n</i>-3 PUFAs) supplementation during neonatal IH reduces the severity of oxygen-induced retinopathy (OIR). Newborn rats were exposed to two IH paradigms: (1) 50% O<sub>2</sub> with brief hypoxia (12% O<sub>2</sub>); or (2) 21% O<sub>2</sub> with brief hypoxia, until postnatal day 14 (P14), during which they received daily oral CoQ10 in olive oil, <i>n</i>-3 PUFAs in fish oil, or olive oil only and compared to room air (RA) treated groups. Pups were examined at P14, or placed in RA until P21. Retinal angiogenesis, histopathology, and morphometry were determined. Both IH paradigms produced severe OIR, but these were worsened with 50/12% O<sub>2</sub> IH. CoQ10 and <i>n</i>-3 PUFAs reduced the severity of OIR, as well as ocular growth factors in both IH paradigms, but CoQ10 was more effective in 50/12% O<sub>2</sub> IH. Supplementation with either CoQ10 or <i>n</i>-3 PUFAs targeting IH-induced retinal injury is individually effective for ameliorating specific characteristics consistent with ROP. Given the complexity of ROP, further studies are needed to determine whether combined CoQ10 and <i>n</i>-3 PUFAs supplementation would optimize their efficacy and result in a better outcome. |
| first_indexed | 2024-03-12T08:04:27Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T08:04:27Z |
| publishDate | 2018-11-01 |
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| series | Antioxidants |
| spelling | doaj.art-f9cc3b52414b461ea68a15304e9e512d2023-09-02T19:43:33ZengMDPI AGAntioxidants2076-39212018-11-0171116010.3390/antiox7110160antiox7110160Comparative Effects of Coenzyme Q10 or <i>n</i>-3 Polyunsaturated Fatty Acid Supplementation on Retinal Angiogenesis in a Rat Model of Oxygen-Induced RetinopathyKay D. Beharry0Charles L. Cai1Faisal Siddiqui2Sara Chowdhury3Christina D’Agrosa4Gloria B. Valencia5Jacob V. Aranda6Department of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY 11203, USADepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY 11203, USADepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY 11203, USADepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY 11203, USADepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY 11203, USADepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY 11203, USADepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY 11203, USANeonatal intermittent hypoxia (IH) or apnea afflicts 70% to 90% of all preterm infants <28 weeks gestation, and is associated with severe retinopathy of prematurity (ROP). We tested the hypotheses that coenzyme Q10 (CoQ10) or omega-3 polyunsaturated fatty acids (<i>n</i>-3 PUFAs) supplementation during neonatal IH reduces the severity of oxygen-induced retinopathy (OIR). Newborn rats were exposed to two IH paradigms: (1) 50% O<sub>2</sub> with brief hypoxia (12% O<sub>2</sub>); or (2) 21% O<sub>2</sub> with brief hypoxia, until postnatal day 14 (P14), during which they received daily oral CoQ10 in olive oil, <i>n</i>-3 PUFAs in fish oil, or olive oil only and compared to room air (RA) treated groups. Pups were examined at P14, or placed in RA until P21. Retinal angiogenesis, histopathology, and morphometry were determined. Both IH paradigms produced severe OIR, but these were worsened with 50/12% O<sub>2</sub> IH. CoQ10 and <i>n</i>-3 PUFAs reduced the severity of OIR, as well as ocular growth factors in both IH paradigms, but CoQ10 was more effective in 50/12% O<sub>2</sub> IH. Supplementation with either CoQ10 or <i>n</i>-3 PUFAs targeting IH-induced retinal injury is individually effective for ameliorating specific characteristics consistent with ROP. Given the complexity of ROP, further studies are needed to determine whether combined CoQ10 and <i>n</i>-3 PUFAs supplementation would optimize their efficacy and result in a better outcome.https://www.mdpi.com/2076-3921/7/11/160coenzyme Q10insulin-like growth factor-I<i>n</i>-3 polyunsaturated fatty acidsneonatal intermittent hypoxiaoxygen-induced retinopathyvascular endothelial growth factor |
| spellingShingle | Kay D. Beharry Charles L. Cai Faisal Siddiqui Sara Chowdhury Christina D’Agrosa Gloria B. Valencia Jacob V. Aranda Comparative Effects of Coenzyme Q10 or <i>n</i>-3 Polyunsaturated Fatty Acid Supplementation on Retinal Angiogenesis in a Rat Model of Oxygen-Induced Retinopathy Antioxidants coenzyme Q10 insulin-like growth factor-I <i>n</i>-3 polyunsaturated fatty acids neonatal intermittent hypoxia oxygen-induced retinopathy vascular endothelial growth factor |
| title | Comparative Effects of Coenzyme Q10 or <i>n</i>-3 Polyunsaturated Fatty Acid Supplementation on Retinal Angiogenesis in a Rat Model of Oxygen-Induced Retinopathy |
| title_full | Comparative Effects of Coenzyme Q10 or <i>n</i>-3 Polyunsaturated Fatty Acid Supplementation on Retinal Angiogenesis in a Rat Model of Oxygen-Induced Retinopathy |
| title_fullStr | Comparative Effects of Coenzyme Q10 or <i>n</i>-3 Polyunsaturated Fatty Acid Supplementation on Retinal Angiogenesis in a Rat Model of Oxygen-Induced Retinopathy |
| title_full_unstemmed | Comparative Effects of Coenzyme Q10 or <i>n</i>-3 Polyunsaturated Fatty Acid Supplementation on Retinal Angiogenesis in a Rat Model of Oxygen-Induced Retinopathy |
| title_short | Comparative Effects of Coenzyme Q10 or <i>n</i>-3 Polyunsaturated Fatty Acid Supplementation on Retinal Angiogenesis in a Rat Model of Oxygen-Induced Retinopathy |
| title_sort | comparative effects of coenzyme q10 or i n i 3 polyunsaturated fatty acid supplementation on retinal angiogenesis in a rat model of oxygen induced retinopathy |
| topic | coenzyme Q10 insulin-like growth factor-I <i>n</i>-3 polyunsaturated fatty acids neonatal intermittent hypoxia oxygen-induced retinopathy vascular endothelial growth factor |
| url | https://www.mdpi.com/2076-3921/7/11/160 |
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