Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo Bioluminescence
Luciferase from the North American firefly ( Photinis pyralis ) is a useful reporter gene in vivo, allowing noninvasive imaging of tumor growth, metastasis, gene transfer, drug treatment, and gene expression. Luciferase is heat labile with an in vitro half-life of approximately 3 min at 37°C. We hav...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publications
2004-10-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200403178 |
| _version_ | 1797283404970983424 |
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| author | Brenda Baggett Rupali Roy Shafinaz Momen Sherif Morgan Laurence Tisi David Morse Robert J. Gillies |
| author_facet | Brenda Baggett Rupali Roy Shafinaz Momen Sherif Morgan Laurence Tisi David Morse Robert J. Gillies |
| author_sort | Brenda Baggett |
| collection | DOAJ |
| description | Luciferase from the North American firefly ( Photinis pyralis ) is a useful reporter gene in vivo, allowing noninvasive imaging of tumor growth, metastasis, gene transfer, drug treatment, and gene expression. Luciferase is heat labile with an in vitro half-life of approximately 3 min at 37°C. We have characterized wild type and six thermostabilized mutant luciferases. In vitro, mutants showed half-lives between 2- and 25-fold higher than wild type. Luciferase transfected mammalian cells were used to determine in vivo half-lives following cycloheximide inhibition of de novo protein synthesis. This showed increased in vivo thermostability in both wild-type and mutant luciferases. This may be due to a variety of factors, including chaperone activity, as steady-state luciferase levels were reduced by geldanamycin, an Hsp90 inhibitor. Mice inoculated with tumor cells stably transfected with mutant or wild-type luciferases were imaged. Increased light production and sensitivity were observed in the tumors bearing thermostable luciferase. Thermostable proteins increase imaging sensitivity. Presumably, as more active protein accumulates, detection is possible from a smaller number of mutant transfected cells compared to wild-type transfected cells. |
| first_indexed | 2024-03-07T17:31:35Z |
| format | Article |
| id | doaj.art-f9cd53b764b94921bd91adf187fa7851 |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T17:31:35Z |
| publishDate | 2004-10-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-f9cd53b764b94921bd91adf187fa78512024-03-02T17:58:06ZengSAGE PublicationsMolecular Imaging1536-01212004-10-01310.1162/1535350020040317810.1162_15353500200403178Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo BioluminescenceBrenda Baggett0Rupali Roy1Shafinaz Momen2Sherif Morgan3Laurence Tisi4David Morse5Robert J. Gillies6University of ArizonaUniversity of ArizonaUniversity of ArizonaUniversity of ArizonaCambridge UniversityUniversity of ArizonaUniversity of ArizonaLuciferase from the North American firefly ( Photinis pyralis ) is a useful reporter gene in vivo, allowing noninvasive imaging of tumor growth, metastasis, gene transfer, drug treatment, and gene expression. Luciferase is heat labile with an in vitro half-life of approximately 3 min at 37°C. We have characterized wild type and six thermostabilized mutant luciferases. In vitro, mutants showed half-lives between 2- and 25-fold higher than wild type. Luciferase transfected mammalian cells were used to determine in vivo half-lives following cycloheximide inhibition of de novo protein synthesis. This showed increased in vivo thermostability in both wild-type and mutant luciferases. This may be due to a variety of factors, including chaperone activity, as steady-state luciferase levels were reduced by geldanamycin, an Hsp90 inhibitor. Mice inoculated with tumor cells stably transfected with mutant or wild-type luciferases were imaged. Increased light production and sensitivity were observed in the tumors bearing thermostable luciferase. Thermostable proteins increase imaging sensitivity. Presumably, as more active protein accumulates, detection is possible from a smaller number of mutant transfected cells compared to wild-type transfected cells.https://doi.org/10.1162/15353500200403178 |
| spellingShingle | Brenda Baggett Rupali Roy Shafinaz Momen Sherif Morgan Laurence Tisi David Morse Robert J. Gillies Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo Bioluminescence Molecular Imaging |
| title | Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo Bioluminescence |
| title_full | Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo Bioluminescence |
| title_fullStr | Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo Bioluminescence |
| title_full_unstemmed | Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo Bioluminescence |
| title_short | Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo Bioluminescence |
| title_sort | thermostability of firefly luciferases affects efficiency of detection by in vivo bioluminescence |
| url | https://doi.org/10.1162/15353500200403178 |
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