Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo Bioluminescence
Luciferase from the North American firefly ( Photinis pyralis ) is a useful reporter gene in vivo, allowing noninvasive imaging of tumor growth, metastasis, gene transfer, drug treatment, and gene expression. Luciferase is heat labile with an in vitro half-life of approximately 3 min at 37°C. We hav...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publications
2004-10-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200403178 |