Evaluation of metabolites in Iranian Licorice accessions under salinity stress and Azotobacter sp. inoculation
Abstract Licorice (Glycyrrhiza glabra L.) is an industrial medicinal plant that is potentially threatened by extinction. In this study, the effects of salinity (0 and 200 mM sodium chloride (NaCl)) and Azotobacter inoculation were evaluated on 16 licorice accessions. The results showed that salinity...
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| Format: | Article |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-20366-6 |
| _version_ | 1828175463559725056 |
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| author | Seyyed Sasan Mousavi Akbar Karami Mohammad Jamal Saharkhiz Mohammad Etemadi Mohammad Mehdi Zarshenas |
| author_facet | Seyyed Sasan Mousavi Akbar Karami Mohammad Jamal Saharkhiz Mohammad Etemadi Mohammad Mehdi Zarshenas |
| author_sort | Seyyed Sasan Mousavi |
| collection | DOAJ |
| description | Abstract Licorice (Glycyrrhiza glabra L.) is an industrial medicinal plant that is potentially threatened by extinction. In this study, the effects of salinity (0 and 200 mM sodium chloride (NaCl)) and Azotobacter inoculation were evaluated on 16 licorice accessions. The results showed that salinity significantly reduced the fresh and dry biomass (FW and DW, respectively) of roots, compared to plants of the control group (a decrease of 15.92% and 17.26%, respectively). As a result of bacterial inoculation, the total sugar content of roots increased by 21.56% when salinity was applied, but increased by 14.01% without salinity. Salinity stress increased the content of glycyrrhizic acid (GA), phenols, and flavonoids in licorice roots by 104.6%, 117.2%, and 56.3%, respectively. Integrated bacterial inoculation and salt stress significantly increased the GA content in the accessions. Bajgah and Sepidan accessions had the highest GA contents (96.26 and 83.17 mg/g DW, respectively), while Eghlid accession had the lowest (41.98 mg/g DW). With the bacterial application, the maximum amounts of glabridin were obtained in Kashmar and Kermanshah accessions (2.04 and 1.98 mg/g DW, respectively). Bajgah and Kashmar accessions had higher amounts of rutin in their aerial parts (6.11 and 9.48 mg/g DW, respectively) when their roots were uninoculated. In conclusion, these results can assist in selecting promising licorice accessions for cultivation in harsh environments. |
| first_indexed | 2024-04-12T04:28:27Z |
| format | Article |
| id | doaj.art-f9d1937c0e1c43eaa08872a537ab4720 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T04:28:27Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f9d1937c0e1c43eaa08872a537ab47202022-12-22T03:48:00ZengNature PortfolioScientific Reports2045-23222022-09-0112111310.1038/s41598-022-20366-6Evaluation of metabolites in Iranian Licorice accessions under salinity stress and Azotobacter sp. inoculationSeyyed Sasan Mousavi0Akbar Karami1Mohammad Jamal Saharkhiz2Mohammad Etemadi3Mohammad Mehdi Zarshenas4Department of Horticultural Science, School of Agriculture, Shiraz UniversityDepartment of Horticultural Science, School of Agriculture, Shiraz UniversityDepartment of Horticultural Science, School of Agriculture, Shiraz UniversityDepartment of Horticultural Science, School of Agriculture, Shiraz UniversityMedicinal Plants Processing Research Center, Shiraz University of Medical SciencesAbstract Licorice (Glycyrrhiza glabra L.) is an industrial medicinal plant that is potentially threatened by extinction. In this study, the effects of salinity (0 and 200 mM sodium chloride (NaCl)) and Azotobacter inoculation were evaluated on 16 licorice accessions. The results showed that salinity significantly reduced the fresh and dry biomass (FW and DW, respectively) of roots, compared to plants of the control group (a decrease of 15.92% and 17.26%, respectively). As a result of bacterial inoculation, the total sugar content of roots increased by 21.56% when salinity was applied, but increased by 14.01% without salinity. Salinity stress increased the content of glycyrrhizic acid (GA), phenols, and flavonoids in licorice roots by 104.6%, 117.2%, and 56.3%, respectively. Integrated bacterial inoculation and salt stress significantly increased the GA content in the accessions. Bajgah and Sepidan accessions had the highest GA contents (96.26 and 83.17 mg/g DW, respectively), while Eghlid accession had the lowest (41.98 mg/g DW). With the bacterial application, the maximum amounts of glabridin were obtained in Kashmar and Kermanshah accessions (2.04 and 1.98 mg/g DW, respectively). Bajgah and Kashmar accessions had higher amounts of rutin in their aerial parts (6.11 and 9.48 mg/g DW, respectively) when their roots were uninoculated. In conclusion, these results can assist in selecting promising licorice accessions for cultivation in harsh environments.https://doi.org/10.1038/s41598-022-20366-6 |
| spellingShingle | Seyyed Sasan Mousavi Akbar Karami Mohammad Jamal Saharkhiz Mohammad Etemadi Mohammad Mehdi Zarshenas Evaluation of metabolites in Iranian Licorice accessions under salinity stress and Azotobacter sp. inoculation Scientific Reports |
| title | Evaluation of metabolites in Iranian Licorice accessions under salinity stress and Azotobacter sp. inoculation |
| title_full | Evaluation of metabolites in Iranian Licorice accessions under salinity stress and Azotobacter sp. inoculation |
| title_fullStr | Evaluation of metabolites in Iranian Licorice accessions under salinity stress and Azotobacter sp. inoculation |
| title_full_unstemmed | Evaluation of metabolites in Iranian Licorice accessions under salinity stress and Azotobacter sp. inoculation |
| title_short | Evaluation of metabolites in Iranian Licorice accessions under salinity stress and Azotobacter sp. inoculation |
| title_sort | evaluation of metabolites in iranian licorice accessions under salinity stress and azotobacter sp inoculation |
| url | https://doi.org/10.1038/s41598-022-20366-6 |
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