Detection of IL-37 in peripheral blood of patients with rheumatoid arthritis and its clinical significance
The study aimed to detect the expression level of interleukin-37 (IL-37) in patients with rheumatoid arthritis (RA) and explore its clinical significance. A total of 40 peripheral blood samples from active and stable RA patients were collected (40 patients with RA), and peripheral blood from 40 heal...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2019-03-01
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| Series: | European Journal of Inflammation |
| Online Access: | https://doi.org/10.1177/2058739218820221 |
| _version_ | 1819113115898347520 |
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| author | Ge Zhang Wei Huang Ying Wang |
| author_facet | Ge Zhang Wei Huang Ying Wang |
| author_sort | Ge Zhang |
| collection | DOAJ |
| description | The study aimed to detect the expression level of interleukin-37 (IL-37) in patients with rheumatoid arthritis (RA) and explore its clinical significance. A total of 40 peripheral blood samples from active and stable RA patients were collected (40 patients with RA), and peripheral blood from 40 healthy volunteers was used as the control group. Peripheral blood serum and peripheral blood mononuclear cells (PBMCs) were isolated. The expression of IL-37 mRNA in PBMCs was detected by real-time fluorescence quantitative PCR. Serum levels of IL-37, rheumatoid factor (RF), and anticyclic citrullinated peptide antibody (CCP) were measured by enzyme-linked immunosorbent assay (ELISA). The results were then calculated and analyzed. The results showed that expression of IL-37 mRNA in the PBMCs of patients with RA was significantly higher than that in the control group ( P < 0.05). Expression of IL-37 mRNA in the PBMCs of the active period group was significantly higher than that in the stable period group ( P < 0.05). IL-37 levels in patients with RA were significantly higher than those of the control group ( P < 0.05). IL-37 levels in the active period group were also significantly higher than those of the stable period group ( P < 0.05). The comparative analysis of RF and anti-CCP antibody levels showed that IL-37 was positively correlated with RF and anti-CCP levels in patients with RA. In conclusion, the expression level of IL-37 in peripheral blood of RA patients was significantly higher than that of normal control group, and it was correlated with RF and CCP antibody levels, indicating that IL-37 plays an important role in the development of RA. |
| first_indexed | 2024-12-22T04:24:17Z |
| format | Article |
| id | doaj.art-f9d2eaf9cb5b4d5d9917409b34fae6c3 |
| institution | Directory Open Access Journal |
| issn | 2058-7392 |
| language | English |
| last_indexed | 2024-12-22T04:24:17Z |
| publishDate | 2019-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | European Journal of Inflammation |
| spelling | doaj.art-f9d2eaf9cb5b4d5d9917409b34fae6c32022-12-21T18:39:11ZengSAGE PublishingEuropean Journal of Inflammation2058-73922019-03-011710.1177/2058739218820221Detection of IL-37 in peripheral blood of patients with rheumatoid arthritis and its clinical significanceGe ZhangWei HuangYing WangThe study aimed to detect the expression level of interleukin-37 (IL-37) in patients with rheumatoid arthritis (RA) and explore its clinical significance. A total of 40 peripheral blood samples from active and stable RA patients were collected (40 patients with RA), and peripheral blood from 40 healthy volunteers was used as the control group. Peripheral blood serum and peripheral blood mononuclear cells (PBMCs) were isolated. The expression of IL-37 mRNA in PBMCs was detected by real-time fluorescence quantitative PCR. Serum levels of IL-37, rheumatoid factor (RF), and anticyclic citrullinated peptide antibody (CCP) were measured by enzyme-linked immunosorbent assay (ELISA). The results were then calculated and analyzed. The results showed that expression of IL-37 mRNA in the PBMCs of patients with RA was significantly higher than that in the control group ( P < 0.05). Expression of IL-37 mRNA in the PBMCs of the active period group was significantly higher than that in the stable period group ( P < 0.05). IL-37 levels in patients with RA were significantly higher than those of the control group ( P < 0.05). IL-37 levels in the active period group were also significantly higher than those of the stable period group ( P < 0.05). The comparative analysis of RF and anti-CCP antibody levels showed that IL-37 was positively correlated with RF and anti-CCP levels in patients with RA. In conclusion, the expression level of IL-37 in peripheral blood of RA patients was significantly higher than that of normal control group, and it was correlated with RF and CCP antibody levels, indicating that IL-37 plays an important role in the development of RA.https://doi.org/10.1177/2058739218820221 |
| spellingShingle | Ge Zhang Wei Huang Ying Wang Detection of IL-37 in peripheral blood of patients with rheumatoid arthritis and its clinical significance European Journal of Inflammation |
| title | Detection of IL-37 in peripheral blood of patients with rheumatoid arthritis and its clinical significance |
| title_full | Detection of IL-37 in peripheral blood of patients with rheumatoid arthritis and its clinical significance |
| title_fullStr | Detection of IL-37 in peripheral blood of patients with rheumatoid arthritis and its clinical significance |
| title_full_unstemmed | Detection of IL-37 in peripheral blood of patients with rheumatoid arthritis and its clinical significance |
| title_short | Detection of IL-37 in peripheral blood of patients with rheumatoid arthritis and its clinical significance |
| title_sort | detection of il 37 in peripheral blood of patients with rheumatoid arthritis and its clinical significance |
| url | https://doi.org/10.1177/2058739218820221 |
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