A GABA Receptor Modulator and Semiochemical Compounds Evidenced Using Volatolomics as Candidate Markers of Chronic Exposure to Fipronil in <i>Apis mellifera</i>
Among the various “omics” approaches that can be used in toxicology, volatolomics is in full development. A volatolomic study was carried out on soil bacteria to validate the proof of concept, and this approach was implemented in a new model organism: the honeybee <i>Apis mellifera</i>....
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/2/185 |
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| author | Vincent Fernandes Kevin Hidalgo Marie Diogon Frédéric Mercier Magaly Angénieux Jérémy Ratel Frédéric Delbac Erwan Engel Philippe Bouchard |
| author_facet | Vincent Fernandes Kevin Hidalgo Marie Diogon Frédéric Mercier Magaly Angénieux Jérémy Ratel Frédéric Delbac Erwan Engel Philippe Bouchard |
| author_sort | Vincent Fernandes |
| collection | DOAJ |
| description | Among the various “omics” approaches that can be used in toxicology, volatolomics is in full development. A volatolomic study was carried out on soil bacteria to validate the proof of concept, and this approach was implemented in a new model organism: the honeybee <i>Apis mellifera</i>. Emerging bees raised in the laboratory in pain-type cages were used. Volatolomics analysis was performed on cuticles, fat bodies, and adhering tissues (abdomens without the digestive tract), after 14 and 21 days of chronic exposure to 0.5 and 1 µg/L of fipronil, corresponding to sublethal doses. The VOCs analysis was processed using an HS-SPME/GC-MS method. A total of 281 features were extracted and tentatively identified. No significant effect of fipronil on the volatolome could be observed after 14 days of chronic exposure. Mainly after 21 days of exposure, a volatolome deviation appeared. The study of this deviation highlighted 11 VOCs whose signal abundances evolved during the experiment. Interestingly, the volatolomics approach revealed a VOC (2,6-dimethylcyclohexanol) that could act on GABA receptor activity (the fipronil target) and VOCs associated with semiochemical activities (pheromones, repellent agents, and compounds related to the Nasonov gland) leading to a potential impact on bee behavior. |
| first_indexed | 2024-03-11T08:26:49Z |
| format | Article |
| id | doaj.art-f9d3ed2531c14e978ede1811c9e25031 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T08:26:49Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-f9d3ed2531c14e978ede1811c9e250312023-11-16T22:04:05ZengMDPI AGMetabolites2218-19892023-01-0113218510.3390/metabo13020185A GABA Receptor Modulator and Semiochemical Compounds Evidenced Using Volatolomics as Candidate Markers of Chronic Exposure to Fipronil in <i>Apis mellifera</i>Vincent Fernandes0Kevin Hidalgo1Marie Diogon2Frédéric Mercier3Magaly Angénieux4Jérémy Ratel5Frédéric Delbac6Erwan Engel7Philippe Bouchard8CNRS, UMR6023, Laboratoire Microorganismes Génome et Environnement, Université Clermont Auvergne, F-63000 Clermont-Ferrand, FranceCNRS, UMR6023, Laboratoire Microorganismes Génome et Environnement, Université Clermont Auvergne, F-63000 Clermont-Ferrand, FranceCNRS, UMR6023, Laboratoire Microorganismes Génome et Environnement, Université Clermont Auvergne, F-63000 Clermont-Ferrand, FranceINRAE, UR370 Qualité des Produites Animaux, MASS Group, F-63122 Saint-Genès-Champanelle, FranceINRAE, UR370 Qualité des Produites Animaux, MASS Group, F-63122 Saint-Genès-Champanelle, FranceINRAE, UR370 Qualité des Produites Animaux, MASS Group, F-63122 Saint-Genès-Champanelle, FranceCNRS, UMR6023, Laboratoire Microorganismes Génome et Environnement, Université Clermont Auvergne, F-63000 Clermont-Ferrand, FranceINRAE, UR370 Qualité des Produites Animaux, MASS Group, F-63122 Saint-Genès-Champanelle, FranceCNRS, UMR6023, Laboratoire Microorganismes Génome et Environnement, Université Clermont Auvergne, F-63000 Clermont-Ferrand, FranceAmong the various “omics” approaches that can be used in toxicology, volatolomics is in full development. A volatolomic study was carried out on soil bacteria to validate the proof of concept, and this approach was implemented in a new model organism: the honeybee <i>Apis mellifera</i>. Emerging bees raised in the laboratory in pain-type cages were used. Volatolomics analysis was performed on cuticles, fat bodies, and adhering tissues (abdomens without the digestive tract), after 14 and 21 days of chronic exposure to 0.5 and 1 µg/L of fipronil, corresponding to sublethal doses. The VOCs analysis was processed using an HS-SPME/GC-MS method. A total of 281 features were extracted and tentatively identified. No significant effect of fipronil on the volatolome could be observed after 14 days of chronic exposure. Mainly after 21 days of exposure, a volatolome deviation appeared. The study of this deviation highlighted 11 VOCs whose signal abundances evolved during the experiment. Interestingly, the volatolomics approach revealed a VOC (2,6-dimethylcyclohexanol) that could act on GABA receptor activity (the fipronil target) and VOCs associated with semiochemical activities (pheromones, repellent agents, and compounds related to the Nasonov gland) leading to a potential impact on bee behavior.https://www.mdpi.com/2218-1989/13/2/185volatolomicsvolatile organic compoundspesticideGABA modulatorhoneybees |
| spellingShingle | Vincent Fernandes Kevin Hidalgo Marie Diogon Frédéric Mercier Magaly Angénieux Jérémy Ratel Frédéric Delbac Erwan Engel Philippe Bouchard A GABA Receptor Modulator and Semiochemical Compounds Evidenced Using Volatolomics as Candidate Markers of Chronic Exposure to Fipronil in <i>Apis mellifera</i> Metabolites volatolomics volatile organic compounds pesticide GABA modulator honeybees |
| title | A GABA Receptor Modulator and Semiochemical Compounds Evidenced Using Volatolomics as Candidate Markers of Chronic Exposure to Fipronil in <i>Apis mellifera</i> |
| title_full | A GABA Receptor Modulator and Semiochemical Compounds Evidenced Using Volatolomics as Candidate Markers of Chronic Exposure to Fipronil in <i>Apis mellifera</i> |
| title_fullStr | A GABA Receptor Modulator and Semiochemical Compounds Evidenced Using Volatolomics as Candidate Markers of Chronic Exposure to Fipronil in <i>Apis mellifera</i> |
| title_full_unstemmed | A GABA Receptor Modulator and Semiochemical Compounds Evidenced Using Volatolomics as Candidate Markers of Chronic Exposure to Fipronil in <i>Apis mellifera</i> |
| title_short | A GABA Receptor Modulator and Semiochemical Compounds Evidenced Using Volatolomics as Candidate Markers of Chronic Exposure to Fipronil in <i>Apis mellifera</i> |
| title_sort | gaba receptor modulator and semiochemical compounds evidenced using volatolomics as candidate markers of chronic exposure to fipronil in i apis mellifera i |
| topic | volatolomics volatile organic compounds pesticide GABA modulator honeybees |
| url | https://www.mdpi.com/2218-1989/13/2/185 |
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