Placental Sampling for Understanding Viral Infections - A Simplified Protocol for the COVID-19 Pandemic
Abstract Objective The coronavirus disease 2019 (COVID-19) is a pandemic viral disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The impact of the disease among the obstetric population remains unclear, and the study of the placenta can provide valuable information....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Federação Brasileira das Sociedades de Ginecologia e Obstetrícia
2021-07-01
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| Series: | Revista Brasileira de Ginecologia e Obstetrícia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032021000500377&tlng=en |
| _version_ | 1798021822122295296 |
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| author | Guilherme de Moraes Nobrega José Paulo Siqueira Guida Rodolfo Rosa Japecanga Arthur Antolini-Tavares Indira Mysorekar Maria Laura Costa |
| author_facet | Guilherme de Moraes Nobrega José Paulo Siqueira Guida Rodolfo Rosa Japecanga Arthur Antolini-Tavares Indira Mysorekar Maria Laura Costa |
| author_sort | Guilherme de Moraes Nobrega |
| collection | DOAJ |
| description | Abstract Objective The coronavirus disease 2019 (COVID-19) is a pandemic viral disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The impact of the disease among the obstetric population remains unclear, and the study of the placenta can provide valuable information. Adequate sampling of the placental tissue can help characterize the pathways of viral infections. Methods A protocol of placental sampling is proposed, aiming at guaranteeing representativity of the placenta and describing the adequate conservation of samples and their integrity for future analysis. The protocol is presented in its complete and simplified versions, allowing its implementation in different complexity settings. Results Sampling with the minimum possible interval from childbirth is the key for adequate sampling and storage. This protocol has already been implemented during the Zika virus outbreak. Conclusion A protocol for adequate sampling and storage of placental tissue is fundamental for adequate evaluation of viral infections on the placenta. During the COVID-19 pandemic, implementation of this protocol may help to elucidate critical aspects of the SARS-CoV-2 infection. |
| first_indexed | 2024-04-11T17:19:42Z |
| format | Article |
| id | doaj.art-f9d41ad2987c45298c6e63ecf90166dd |
| institution | Directory Open Access Journal |
| issn | 0100-7203 |
| language | English |
| last_indexed | 2024-04-11T17:19:42Z |
| publishDate | 2021-07-01 |
| publisher | Federação Brasileira das Sociedades de Ginecologia e Obstetrícia |
| record_format | Article |
| series | Revista Brasileira de Ginecologia e Obstetrícia |
| spelling | doaj.art-f9d41ad2987c45298c6e63ecf90166dd2022-12-22T04:12:32ZengFederação Brasileira das Sociedades de Ginecologia e ObstetríciaRevista Brasileira de Ginecologia e Obstetrícia0100-72032021-07-0143537738310.1055/s-0041-1729146Placental Sampling for Understanding Viral Infections - A Simplified Protocol for the COVID-19 PandemicGuilherme de Moraes Nobregahttps://orcid.org/0000-0001-9406-4256José Paulo Siqueira Guidahttps://orcid.org/0000-0002-3648-6159Rodolfo Rosa Japecangahttps://orcid.org/0000-0002-1611-5174Arthur Antolini-Tavareshttps://orcid.org/0000-0001-5964-4407Indira Mysorekarhttps://orcid.org/0000-0003-4539-9257Maria Laura Costahttps://orcid.org/0000-0001-8280-3234Abstract Objective The coronavirus disease 2019 (COVID-19) is a pandemic viral disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The impact of the disease among the obstetric population remains unclear, and the study of the placenta can provide valuable information. Adequate sampling of the placental tissue can help characterize the pathways of viral infections. Methods A protocol of placental sampling is proposed, aiming at guaranteeing representativity of the placenta and describing the adequate conservation of samples and their integrity for future analysis. The protocol is presented in its complete and simplified versions, allowing its implementation in different complexity settings. Results Sampling with the minimum possible interval from childbirth is the key for adequate sampling and storage. This protocol has already been implemented during the Zika virus outbreak. Conclusion A protocol for adequate sampling and storage of placental tissue is fundamental for adequate evaluation of viral infections on the placenta. During the COVID-19 pandemic, implementation of this protocol may help to elucidate critical aspects of the SARS-CoV-2 infection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032021000500377&tlng=enCOVID-19placentapregnancysystematic samplingviral infections |
| spellingShingle | Guilherme de Moraes Nobrega José Paulo Siqueira Guida Rodolfo Rosa Japecanga Arthur Antolini-Tavares Indira Mysorekar Maria Laura Costa Placental Sampling for Understanding Viral Infections - A Simplified Protocol for the COVID-19 Pandemic Revista Brasileira de Ginecologia e Obstetrícia COVID-19 placenta pregnancy systematic sampling viral infections |
| title | Placental Sampling for Understanding Viral Infections - A Simplified Protocol for the COVID-19 Pandemic |
| title_full | Placental Sampling for Understanding Viral Infections - A Simplified Protocol for the COVID-19 Pandemic |
| title_fullStr | Placental Sampling for Understanding Viral Infections - A Simplified Protocol for the COVID-19 Pandemic |
| title_full_unstemmed | Placental Sampling for Understanding Viral Infections - A Simplified Protocol for the COVID-19 Pandemic |
| title_short | Placental Sampling for Understanding Viral Infections - A Simplified Protocol for the COVID-19 Pandemic |
| title_sort | placental sampling for understanding viral infections a simplified protocol for the covid 19 pandemic |
| topic | COVID-19 placenta pregnancy systematic sampling viral infections |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032021000500377&tlng=en |
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