<i>Lactobacillus</i> Probiotic Strains Differ in Their Ability to Adhere to Human Lung Epithelial Cells and to Prevent Adhesion of Clinical Isolates of <i>Pseudomonas aeruginosa</i> from Cystic Fibrosis Lung
The field of probiotic applications is rapidly expanding, including their use for the control of respiratory tract infections. Nevertheless, probiotics ability to colonize the lung environment and to compete with pulmonary pathogens is still a poorly investigated research area. In this study, we aim...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/7/1707 |
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| author | Giovanna Batoni Esingül Kaya Elisa Catelli Sabrina Quinti Matteo Botti Alessandro De Carli Marta Bianchi Giuseppantonio Maisetta Semih Esin |
| author_facet | Giovanna Batoni Esingül Kaya Elisa Catelli Sabrina Quinti Matteo Botti Alessandro De Carli Marta Bianchi Giuseppantonio Maisetta Semih Esin |
| author_sort | Giovanna Batoni |
| collection | DOAJ |
| description | The field of probiotic applications is rapidly expanding, including their use for the control of respiratory tract infections. Nevertheless, probiotics ability to colonize the lung environment and to compete with pulmonary pathogens is still a poorly investigated research area. In this study, we aimed to evaluate the adhesion ability of a number of commercial probiotic strains to the human lung epithelial cell line A549. Furthermore, we assessed probiotic ability to prevent host cell adhesion of one of the major lung pathogens in cystic fibrosis, <i>Pseudomonas aeruginosa</i>, and to reduce the pathogen-induced inflammatory response of human peripheral blood mononuclear cells (PBMCs) in terms of cytokine release. <i>Lactobacillus acidophilus</i> displayed the highest adhesion ability to A549 cells evaluated as percent of adhered bacteria compared to the inoculum. In agreement with such an observation, <i>L. acidophilus</i> was the most efficient in preventing adhesion to A549 cells of a <i>P. aeruginosa</i> isolate from CF sputum. Three-color fluorescence labeling of A549 cells, <i>P. aeruginosa</i>, and <i>L. acidophilus</i>, and confocal microcopy image analyses revealed a likely exclusion effect played by both live and UV-killed <i>L. acidophilus</i> towards <i>P. aeruginosa</i>. Such results were confirmed by CFU count. When co-cultured with PBMCs, both live and UV-killed <i>L. acidophilus</i> reduced the amount of IL-1β and IL-6 in culture supernatants in a statistically significant manner. Overall, the results obtained point to <i>L. acidophilus</i> as an interesting candidate for further studies for a potential aerogenous administration to control <i>P. aeruginosa</i> infections. |
| first_indexed | 2024-03-11T00:49:24Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T00:49:24Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f9d5535f7fe34383b0fb3923a9f93f172023-11-18T20:35:19ZengMDPI AGMicroorganisms2076-26072023-06-01117170710.3390/microorganisms11071707<i>Lactobacillus</i> Probiotic Strains Differ in Their Ability to Adhere to Human Lung Epithelial Cells and to Prevent Adhesion of Clinical Isolates of <i>Pseudomonas aeruginosa</i> from Cystic Fibrosis LungGiovanna Batoni0Esingül Kaya1Elisa Catelli2Sabrina Quinti3Matteo Botti4Alessandro De Carli5Marta Bianchi6Giuseppantonio Maisetta7Semih Esin8Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56123 Pisa, ItalyCystic Fibrosis Supporting Service, Azienda USL Toscana Nord-Ovest, 57128 Livorno, ItalyCystic Fibrosis Supporting Service, Azienda USL Toscana Nord-Ovest, 57128 Livorno, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56123 Pisa, ItalyThe field of probiotic applications is rapidly expanding, including their use for the control of respiratory tract infections. Nevertheless, probiotics ability to colonize the lung environment and to compete with pulmonary pathogens is still a poorly investigated research area. In this study, we aimed to evaluate the adhesion ability of a number of commercial probiotic strains to the human lung epithelial cell line A549. Furthermore, we assessed probiotic ability to prevent host cell adhesion of one of the major lung pathogens in cystic fibrosis, <i>Pseudomonas aeruginosa</i>, and to reduce the pathogen-induced inflammatory response of human peripheral blood mononuclear cells (PBMCs) in terms of cytokine release. <i>Lactobacillus acidophilus</i> displayed the highest adhesion ability to A549 cells evaluated as percent of adhered bacteria compared to the inoculum. In agreement with such an observation, <i>L. acidophilus</i> was the most efficient in preventing adhesion to A549 cells of a <i>P. aeruginosa</i> isolate from CF sputum. Three-color fluorescence labeling of A549 cells, <i>P. aeruginosa</i>, and <i>L. acidophilus</i>, and confocal microcopy image analyses revealed a likely exclusion effect played by both live and UV-killed <i>L. acidophilus</i> towards <i>P. aeruginosa</i>. Such results were confirmed by CFU count. When co-cultured with PBMCs, both live and UV-killed <i>L. acidophilus</i> reduced the amount of IL-1β and IL-6 in culture supernatants in a statistically significant manner. Overall, the results obtained point to <i>L. acidophilus</i> as an interesting candidate for further studies for a potential aerogenous administration to control <i>P. aeruginosa</i> infections.https://www.mdpi.com/2076-2607/11/7/1707<i>Lactobacillus</i>cystic fibrosis<i>Pseudomonas aeruginosa</i>adhesion assayimmunomodulationprobiotics |
| spellingShingle | Giovanna Batoni Esingül Kaya Elisa Catelli Sabrina Quinti Matteo Botti Alessandro De Carli Marta Bianchi Giuseppantonio Maisetta Semih Esin <i>Lactobacillus</i> Probiotic Strains Differ in Their Ability to Adhere to Human Lung Epithelial Cells and to Prevent Adhesion of Clinical Isolates of <i>Pseudomonas aeruginosa</i> from Cystic Fibrosis Lung Microorganisms <i>Lactobacillus</i> cystic fibrosis <i>Pseudomonas aeruginosa</i> adhesion assay immunomodulation probiotics |
| title | <i>Lactobacillus</i> Probiotic Strains Differ in Their Ability to Adhere to Human Lung Epithelial Cells and to Prevent Adhesion of Clinical Isolates of <i>Pseudomonas aeruginosa</i> from Cystic Fibrosis Lung |
| title_full | <i>Lactobacillus</i> Probiotic Strains Differ in Their Ability to Adhere to Human Lung Epithelial Cells and to Prevent Adhesion of Clinical Isolates of <i>Pseudomonas aeruginosa</i> from Cystic Fibrosis Lung |
| title_fullStr | <i>Lactobacillus</i> Probiotic Strains Differ in Their Ability to Adhere to Human Lung Epithelial Cells and to Prevent Adhesion of Clinical Isolates of <i>Pseudomonas aeruginosa</i> from Cystic Fibrosis Lung |
| title_full_unstemmed | <i>Lactobacillus</i> Probiotic Strains Differ in Their Ability to Adhere to Human Lung Epithelial Cells and to Prevent Adhesion of Clinical Isolates of <i>Pseudomonas aeruginosa</i> from Cystic Fibrosis Lung |
| title_short | <i>Lactobacillus</i> Probiotic Strains Differ in Their Ability to Adhere to Human Lung Epithelial Cells and to Prevent Adhesion of Clinical Isolates of <i>Pseudomonas aeruginosa</i> from Cystic Fibrosis Lung |
| title_sort | i lactobacillus i probiotic strains differ in their ability to adhere to human lung epithelial cells and to prevent adhesion of clinical isolates of i pseudomonas aeruginosa i from cystic fibrosis lung |
| topic | <i>Lactobacillus</i> cystic fibrosis <i>Pseudomonas aeruginosa</i> adhesion assay immunomodulation probiotics |
| url | https://www.mdpi.com/2076-2607/11/7/1707 |
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