Hybrid Impedimetric Biosensors for Express Protein Markers Detection
Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/15/2/181 |
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| author | Nikita Sitkov Andrey Ryabko Vyacheslav Moshnikov Andrey Aleshin Dmitry Kaplun Tatiana Zimina |
| author_facet | Nikita Sitkov Andrey Ryabko Vyacheslav Moshnikov Andrey Aleshin Dmitry Kaplun Tatiana Zimina |
| author_sort | Nikita Sitkov |
| collection | DOAJ |
| description | Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics. |
| first_indexed | 2024-03-07T22:21:24Z |
| format | Article |
| id | doaj.art-f9d82264ccd440f398c6b4ffa435fc53 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-07T22:21:24Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-f9d82264ccd440f398c6b4ffa435fc532024-02-23T15:27:32ZengMDPI AGMicromachines2072-666X2024-01-0115218110.3390/mi15020181Hybrid Impedimetric Biosensors for Express Protein Markers DetectionNikita Sitkov0Andrey Ryabko1Vyacheslav Moshnikov2Andrey Aleshin3Dmitry Kaplun4Tatiana Zimina5Department of Micro and Nanoelectronics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, RussiaLaboratory of Nonequilibrium Processes in Semiconductors, Ioffe Institute, 26 Politekhnicheskaya, 194021 Saint Petersburg, RussiaDepartment of Micro and Nanoelectronics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, RussiaLaboratory of Nonequilibrium Processes in Semiconductors, Ioffe Institute, 26 Politekhnicheskaya, 194021 Saint Petersburg, RussiaArtificial Intelligence Research Institute, China University of Mining and Technology, 1 Daxue Road, Xuzhou 221116, ChinaDepartment of Micro and Nanoelectronics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, RussiaImpedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.https://www.mdpi.com/2072-666X/15/2/181impedimetric biosensorselectrochemical impedance spectroscopyproteinsnanomaterialsexpress detectionmicrofluidics |
| spellingShingle | Nikita Sitkov Andrey Ryabko Vyacheslav Moshnikov Andrey Aleshin Dmitry Kaplun Tatiana Zimina Hybrid Impedimetric Biosensors for Express Protein Markers Detection Micromachines impedimetric biosensors electrochemical impedance spectroscopy proteins nanomaterials express detection microfluidics |
| title | Hybrid Impedimetric Biosensors for Express Protein Markers Detection |
| title_full | Hybrid Impedimetric Biosensors for Express Protein Markers Detection |
| title_fullStr | Hybrid Impedimetric Biosensors for Express Protein Markers Detection |
| title_full_unstemmed | Hybrid Impedimetric Biosensors for Express Protein Markers Detection |
| title_short | Hybrid Impedimetric Biosensors for Express Protein Markers Detection |
| title_sort | hybrid impedimetric biosensors for express protein markers detection |
| topic | impedimetric biosensors electrochemical impedance spectroscopy proteins nanomaterials express detection microfluidics |
| url | https://www.mdpi.com/2072-666X/15/2/181 |
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