Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test
Purpose: Betulin is a pentacyclic triterpene found in the outer barks of innumerous plants. This secondary metabolite is easily isolated from plants with the major interest in converting it to betulinic acid, which pharmacological properties were much more exploited than betulin. But, investments in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2016-09-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-443.pdf |
| _version_ | 1818833030032130048 |
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| author | Edson Hideaki Yoshida Natália Tribuiani Giovana Sabadim Débora Antunes Neto Moreno Eliana Aparecida Varanda Yoko Oshima-Franco |
| author_facet | Edson Hideaki Yoshida Natália Tribuiani Giovana Sabadim Débora Antunes Neto Moreno Eliana Aparecida Varanda Yoko Oshima-Franco |
| author_sort | Edson Hideaki Yoshida |
| collection | DOAJ |
| description | Purpose: Betulin is a pentacyclic triterpene found in the outer barks of innumerous plants. This secondary metabolite is easily isolated from plants with the major interest in converting it to betulinic acid, which pharmacological properties were much more exploited than betulin. But, investments in the own betulin have been grown since no chemical step is necessary. In this study we focused the precursor betulin in order to evaluate its mutagenicity by Salmonella/microsome assay (Ames test). Methods: The Ames test was carried out using a commercial betulin exposed to Salmonella typhimurium strains TA98, TA100, TA102, and TA97a, in experiments with (+S9) and without (-S9) metabolic activation. Results: Betulin was unable to increase the number of revertants (+S9 and -S9 metabolic activation) showing the absence of any mutagenic effect by Ames test.Conclusion: This study allowed attribute safety to betulin being important for exploiting its pharmacological uses. |
| first_indexed | 2024-12-19T02:12:26Z |
| format | Article |
| id | doaj.art-f9da1f3a50054ba2b6ccb746d1ac1429 |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-19T02:12:26Z |
| publishDate | 2016-09-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-f9da1f3a50054ba2b6ccb746d1ac14292022-12-21T20:40:40ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082016-09-016344344710.15171/apb.2016.057APB_6394_20160622165736Evaluation of Betulin Mutagenicity by Salmonella/Microsome TestEdson Hideaki Yoshida0Natália Tribuiani1Giovana Sabadim2Débora Antunes Neto Moreno3Eliana Aparecida Varanda4Yoko Oshima-Franco5Post-Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil. Post-Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil. Post-Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil. Post-Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil. Pharmaceutical Sciences Faculty of Araraquara, São Paulo State University (UNESP), Rodovia Araraquara-Jau, Km 1, CEP 14801-902, Araraquara, São Paulo, Brazil.Post-Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil. Purpose: Betulin is a pentacyclic triterpene found in the outer barks of innumerous plants. This secondary metabolite is easily isolated from plants with the major interest in converting it to betulinic acid, which pharmacological properties were much more exploited than betulin. But, investments in the own betulin have been grown since no chemical step is necessary. In this study we focused the precursor betulin in order to evaluate its mutagenicity by Salmonella/microsome assay (Ames test). Methods: The Ames test was carried out using a commercial betulin exposed to Salmonella typhimurium strains TA98, TA100, TA102, and TA97a, in experiments with (+S9) and without (-S9) metabolic activation. Results: Betulin was unable to increase the number of revertants (+S9 and -S9 metabolic activation) showing the absence of any mutagenic effect by Ames test.Conclusion: This study allowed attribute safety to betulin being important for exploiting its pharmacological uses.http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-443.pdfAmes testBetulinSalmonella mutagenicityTriterpenoid |
| spellingShingle | Edson Hideaki Yoshida Natália Tribuiani Giovana Sabadim Débora Antunes Neto Moreno Eliana Aparecida Varanda Yoko Oshima-Franco Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test Advanced Pharmaceutical Bulletin Ames test Betulin Salmonella mutagenicity Triterpenoid |
| title | Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test |
| title_full | Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test |
| title_fullStr | Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test |
| title_full_unstemmed | Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test |
| title_short | Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test |
| title_sort | evaluation of betulin mutagenicity by salmonella microsome test |
| topic | Ames test Betulin Salmonella mutagenicity Triterpenoid |
| url | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-443.pdf |
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