A “Green” Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in <em>Croton Crassifolius</em>
Clerodane diterpenoids are the main bioactive constituents of <i>Croton crassifolius</i> and are proved to have multiple biological activities. However, quality control (QC) research on the constituents are rare. Thus, the major research purpose of the current study was to establish an e...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/4/694 |
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| author | Jin-Long Tian Chi Shu Ye Zhang Hui-Jun Cui Xu Xie Xu-Long Ran Tian-Shun Chen Zhi-Huan Zang Jian-Guo Liu Bin Li |
| author_facet | Jin-Long Tian Chi Shu Ye Zhang Hui-Jun Cui Xu Xie Xu-Long Ran Tian-Shun Chen Zhi-Huan Zang Jian-Guo Liu Bin Li |
| author_sort | Jin-Long Tian |
| collection | DOAJ |
| description | Clerodane diterpenoids are the main bioactive constituents of <i>Croton crassifolius</i> and are proved to have multiple biological activities. However, quality control (QC) research on the constituents are rare. Thus, the major research purpose of the current study was to establish an efficient homogenate extraction (HGE) process combined with a sensitive and specific ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS) technique together for the rapid extraction and determination of clerodane diterpenoids in <i>C. crassifolius</i>. All calibration curves showed good linearity (<i>r</i> > 0.9943) within the test ranges and the intra- and inter-day precisions and repeatability were all within required limits. This modified HGE–UHPLC–MS method only took 5 min to extract nine clerodane diterpenoids in <i>C. crassifolius</i> and another 12 min to quantify these components. The results indicated that the quantitative analysis based on UHPLC–MS was a feasible method for QC of clerodane diterpenoids in <i>C. crassifolius</i>, and the findings outlined in the current study also inferred the potential of the method in the QC of clerodane diterpenoids in other complex species of plants. |
| first_indexed | 2024-12-21T20:24:46Z |
| format | Article |
| id | doaj.art-f9db83197de044478ce120deb88423c1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T20:24:46Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f9db83197de044478ce120deb88423c12022-12-21T18:51:24ZengMDPI AGMolecules1420-30492019-02-0124469410.3390/molecules24040694molecules24040694A “Green” Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in <em>Croton Crassifolius</em>Jin-Long Tian0Chi Shu1Ye Zhang2Hui-Jun Cui3Xu Xie4Xu-Long Ran5Tian-Shun Chen6Zhi-Huan Zang7Jian-Guo Liu8Bin Li9College of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaClerodane diterpenoids are the main bioactive constituents of <i>Croton crassifolius</i> and are proved to have multiple biological activities. However, quality control (QC) research on the constituents are rare. Thus, the major research purpose of the current study was to establish an efficient homogenate extraction (HGE) process combined with a sensitive and specific ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS) technique together for the rapid extraction and determination of clerodane diterpenoids in <i>C. crassifolius</i>. All calibration curves showed good linearity (<i>r</i> > 0.9943) within the test ranges and the intra- and inter-day precisions and repeatability were all within required limits. This modified HGE–UHPLC–MS method only took 5 min to extract nine clerodane diterpenoids in <i>C. crassifolius</i> and another 12 min to quantify these components. The results indicated that the quantitative analysis based on UHPLC–MS was a feasible method for QC of clerodane diterpenoids in <i>C. crassifolius</i>, and the findings outlined in the current study also inferred the potential of the method in the QC of clerodane diterpenoids in other complex species of plants.https://www.mdpi.com/1420-3049/24/4/694<i>Croton crassifolius</i>clerodane diterpenoidshomogenate extractionUHPLC–MSprinciple component analysis |
| spellingShingle | Jin-Long Tian Chi Shu Ye Zhang Hui-Jun Cui Xu Xie Xu-Long Ran Tian-Shun Chen Zhi-Huan Zang Jian-Guo Liu Bin Li A “Green” Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in <em>Croton Crassifolius</em> Molecules <i>Croton crassifolius</i> clerodane diterpenoids homogenate extraction UHPLC–MS principle component analysis |
| title | A “Green” Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in <em>Croton Crassifolius</em> |
| title_full | A “Green” Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in <em>Croton Crassifolius</em> |
| title_fullStr | A “Green” Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in <em>Croton Crassifolius</em> |
| title_full_unstemmed | A “Green” Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in <em>Croton Crassifolius</em> |
| title_short | A “Green” Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in <em>Croton Crassifolius</em> |
| title_sort | green homogenate extraction coupled with uhplc ms for the rapid determination of diterpenoids in em croton crassifolius em |
| topic | <i>Croton crassifolius</i> clerodane diterpenoids homogenate extraction UHPLC–MS principle component analysis |
| url | https://www.mdpi.com/1420-3049/24/4/694 |
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