Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran
Background: Leishmaniasis is a protozoan disease cause by Leishmania genus. Anthroponotic and zoonotic cutaneous leishmaniasis are endemic in Iran. The aim of this study was to identify the causative agent of cutaneous leishmaniasis by mini-exon gene in five regions of Khuzestan Province, southwest...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2010-03-01
|
| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/123 |
| _version_ | 1818877363625132032 |
|---|---|
| author | J Saki AR Meamar H Oormazdi L Akhlaghi S Maraghi M Mohebali S Khademvatan E Razmjou |
| author_facet | J Saki AR Meamar H Oormazdi L Akhlaghi S Maraghi M Mohebali S Khademvatan E Razmjou |
| author_sort | J Saki |
| collection | DOAJ |
| description | Background: Leishmaniasis is a protozoan disease cause by Leishmania genus. Anthroponotic and zoonotic cutaneous leishmaniasis are endemic in Iran. The aim of this study was to identify the causative agent of cutaneous leishmaniasis by mini-exon gene in five regions of Khuzestan Province, southwest of Iran.
Methods: From 2007 to 2008 in this cross-sectional study, cutaneous samples were collected from patients referred to Health Centers and Hospitals of the Khuzestan Province for cutaneous leishmaniasis diagnosis and cultured in Novy-MacNeal-Nicolle (NNN) and RPMI 1640. The propagated promastigotes were harvested and Leishmania species of cutaneous leishmaniasis were identified by RFLP and DNA sequencing of the PCR generated fragments.
Results: L. major and L. tropica were the causative agents of cutaneous leishmaniasis by predominantly of L. major species. The alignment of the mini-exon sequencing isolates with reported sequencing of L. major and L. tropica revealed 92%-99% identity.
Conclusion: Our study showed that mini-exon PCR-RFLP was useful method to identify the causative species of cutaneous leishmaniasis. |
| first_indexed | 2024-12-19T13:57:06Z |
| format | Article |
| id | doaj.art-f9dd1b6fa5ea40eeac52a7b4145974d6 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-19T13:57:06Z |
| publishDate | 2010-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-f9dd1b6fa5ea40eeac52a7b4145974d62022-12-21T20:18:34ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2010-03-0151Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest IranJ Saki0AR Meamar1H Oormazdi2L Akhlaghi3S Maraghi4M Mohebali5S Khademvatan6E Razmjou7Department of Medical Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran AND Department of Medical Parasitology and Mycology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Medical Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Medical Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Medical Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Medical Parasitology and Mycology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Parasitology and Mycology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Medical Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranBackground: Leishmaniasis is a protozoan disease cause by Leishmania genus. Anthroponotic and zoonotic cutaneous leishmaniasis are endemic in Iran. The aim of this study was to identify the causative agent of cutaneous leishmaniasis by mini-exon gene in five regions of Khuzestan Province, southwest of Iran. Methods: From 2007 to 2008 in this cross-sectional study, cutaneous samples were collected from patients referred to Health Centers and Hospitals of the Khuzestan Province for cutaneous leishmaniasis diagnosis and cultured in Novy-MacNeal-Nicolle (NNN) and RPMI 1640. The propagated promastigotes were harvested and Leishmania species of cutaneous leishmaniasis were identified by RFLP and DNA sequencing of the PCR generated fragments. Results: L. major and L. tropica were the causative agents of cutaneous leishmaniasis by predominantly of L. major species. The alignment of the mini-exon sequencing isolates with reported sequencing of L. major and L. tropica revealed 92%-99% identity. Conclusion: Our study showed that mini-exon PCR-RFLP was useful method to identify the causative species of cutaneous leishmaniasis.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/123Cutaneous leishmaniasisMmini-exonRFLPSequencingIran |
| spellingShingle | J Saki AR Meamar H Oormazdi L Akhlaghi S Maraghi M Mohebali S Khademvatan E Razmjou Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran Iranian Journal of Parasitology Cutaneous leishmaniasis Mmini-exon RFLP Sequencing Iran |
| title | Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran |
| title_full | Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran |
| title_fullStr | Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran |
| title_full_unstemmed | Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran |
| title_short | Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran |
| title_sort | mini exon genotyping of leishmania species in khuzestan province southwest iran |
| topic | Cutaneous leishmaniasis Mmini-exon RFLP Sequencing Iran |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/123 |
| work_keys_str_mv | AT jsaki miniexongenotypingofleishmaniaspeciesinkhuzestanprovincesouthwestiran AT armeamar miniexongenotypingofleishmaniaspeciesinkhuzestanprovincesouthwestiran AT hoormazdi miniexongenotypingofleishmaniaspeciesinkhuzestanprovincesouthwestiran AT lakhlaghi miniexongenotypingofleishmaniaspeciesinkhuzestanprovincesouthwestiran AT smaraghi miniexongenotypingofleishmaniaspeciesinkhuzestanprovincesouthwestiran AT mmohebali miniexongenotypingofleishmaniaspeciesinkhuzestanprovincesouthwestiran AT skhademvatan miniexongenotypingofleishmaniaspeciesinkhuzestanprovincesouthwestiran AT erazmjou miniexongenotypingofleishmaniaspeciesinkhuzestanprovincesouthwestiran |