Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in Cereals
The primary pollutants in cereal products are aflatoxins B1 (AFB1), zearalenone (ZEN), and deoxynivalenol (DON). In this study, anti-AFB1 MAb (4C9), anti-ZEN MAb (2A3), and anti-DON MAb (1F10) were developed and used in time-resolved fluorescence immunoassay microfluidics to determine AFB1, ZEN, and...
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MDPI AG
2022-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/9/1319 |
| _version_ | 1797504881819385856 |
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| author | Xu Wang Disha Lu Qingfeng Huang Jinyi Yang |
| author_facet | Xu Wang Disha Lu Qingfeng Huang Jinyi Yang |
| author_sort | Xu Wang |
| collection | DOAJ |
| description | The primary pollutants in cereal products are aflatoxins B1 (AFB1), zearalenone (ZEN), and deoxynivalenol (DON). In this study, anti-AFB1 MAb (4C9), anti-ZEN MAb (2A3), and anti-DON MAb (1F10) were developed and used in time-resolved fluorescence immunoassay microfluidics to determine AFB1, ZEN, and DON in agricultural products. The linear range for AFB1, ZEN, and DON were 0.05~2.2 μg/kg, 1.45~375.75 μg/kg, and 11.1~124.2 μg/kg, respectively. In maize, the recoveries of AFB1/ZEN/DON were 92~101%, 102~105%, and 103~108%, respectively. High-performance liquid chromatography and the proposed approach had a good correlation. Time-resolved fluorescence immunoassay microfluidics is a highly efficient and sensitive field detection method for fungal toxins in agricultural products. |
| first_indexed | 2024-03-10T04:10:42Z |
| format | Article |
| id | doaj.art-f9dfaf8d63f442e98e101b05fe81d4ac |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T04:10:42Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-f9dfaf8d63f442e98e101b05fe81d4ac2023-11-23T08:13:47ZengMDPI AGFoods2304-81582022-04-01119131910.3390/foods11091319Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in CerealsXu Wang0Disha Lu1Qingfeng Huang2Jinyi Yang3Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaThe primary pollutants in cereal products are aflatoxins B1 (AFB1), zearalenone (ZEN), and deoxynivalenol (DON). In this study, anti-AFB1 MAb (4C9), anti-ZEN MAb (2A3), and anti-DON MAb (1F10) were developed and used in time-resolved fluorescence immunoassay microfluidics to determine AFB1, ZEN, and DON in agricultural products. The linear range for AFB1, ZEN, and DON were 0.05~2.2 μg/kg, 1.45~375.75 μg/kg, and 11.1~124.2 μg/kg, respectively. In maize, the recoveries of AFB1/ZEN/DON were 92~101%, 102~105%, and 103~108%, respectively. High-performance liquid chromatography and the proposed approach had a good correlation. Time-resolved fluorescence immunoassay microfluidics is a highly efficient and sensitive field detection method for fungal toxins in agricultural products.https://www.mdpi.com/2304-8158/11/9/1319aflatoxins B1zearalenonedeoxynivalenolmonoclonal antibodymicrofluidicstime-resolved fluorescence immunoassay |
| spellingShingle | Xu Wang Disha Lu Qingfeng Huang Jinyi Yang Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in Cereals Foods aflatoxins B1 zearalenone deoxynivalenol monoclonal antibody microfluidics time-resolved fluorescence immunoassay |
| title | Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in Cereals |
| title_full | Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in Cereals |
| title_fullStr | Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in Cereals |
| title_full_unstemmed | Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in Cereals |
| title_short | Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in Cereals |
| title_sort | microfluidics based time resolved fluorescence immunoassay for the on site detection of aflatoxins b1 zearalenone and deoxynivalenol in cereals |
| topic | aflatoxins B1 zearalenone deoxynivalenol monoclonal antibody microfluidics time-resolved fluorescence immunoassay |
| url | https://www.mdpi.com/2304-8158/11/9/1319 |
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