MicroRNA miR-263b-5p Regulates Developmental Growth and Cell Association by Suppressing <i>Laminin A</i> in <i>Drosophila</i>
Basement membranes (BMs) play important roles under various physiological conditions in animals, including ecdysozoans. During development, BMs undergo alterations through diverse intrinsic and extrinsic regulatory mechanisms; however, the full complement of pathways controlling these changes remain...
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/2079-7737/12/8/1096 |
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| author | Chae Jeong Kim Hyun Ho Kim Hee Kyung Kim Sojeong Lee Daegyu Jang Chanhyeok Kim Do-Hwan Lim |
| author_facet | Chae Jeong Kim Hyun Ho Kim Hee Kyung Kim Sojeong Lee Daegyu Jang Chanhyeok Kim Do-Hwan Lim |
| author_sort | Chae Jeong Kim |
| collection | DOAJ |
| description | Basement membranes (BMs) play important roles under various physiological conditions in animals, including ecdysozoans. During development, BMs undergo alterations through diverse intrinsic and extrinsic regulatory mechanisms; however, the full complement of pathways controlling these changes remain unclear. Here, we found that fat body-overexpression of <i>Drosophila miR-263b</i>, which is highly expressed during the larval-to-pupal transition, resulted in a decrease in the overall size of the larval fat body, and ultimately, in a severe growth defect accompanied by a reduction in cell proliferation and cell size. Interestingly, we further observed that a large proportion of the larval fat body cells were prematurely disassociated from each other. Moreover, we present evidence that miR-263b-5p suppresses the main component of BMs, <i>Laminin A</i> (<i>LanA</i>). Through experiments using RNA interference (RNAi) of <i>LanA</i>, we found that its depletion phenocopied the effects in <i>miR-263b</i>-overexpressing flies. Overall, our findings suggest a potential role for miR-263b in developmental growth and cell association by suppressing <i>LanA</i> expression in the <i>Drosophila</i> fat body. |
| first_indexed | 2024-03-09T10:56:50Z |
| format | Article |
| id | doaj.art-f9e070c6834944f7ab2dba5596103fa6 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T10:56:50Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-f9e070c6834944f7ab2dba5596103fa62023-12-01T01:38:55ZengMDPI AGBiology2079-77372023-08-01128109610.3390/biology12081096MicroRNA miR-263b-5p Regulates Developmental Growth and Cell Association by Suppressing <i>Laminin A</i> in <i>Drosophila</i>Chae Jeong Kim0Hyun Ho Kim1Hee Kyung Kim2Sojeong Lee3Daegyu Jang4Chanhyeok Kim5Do-Hwan Lim6School of Systems Biomedical Science, Soongsil University, Seoul 06978, Republic of KoreaSchool of Systems Biomedical Science, Soongsil University, Seoul 06978, Republic of KoreaSchool of Systems Biomedical Science, Soongsil University, Seoul 06978, Republic of KoreaSchool of Systems Biomedical Science, Soongsil University, Seoul 06978, Republic of KoreaSchool of Systems Biomedical Science, Soongsil University, Seoul 06978, Republic of KoreaSchool of Systems Biomedical Science, Soongsil University, Seoul 06978, Republic of KoreaSchool of Systems Biomedical Science, Soongsil University, Seoul 06978, Republic of KoreaBasement membranes (BMs) play important roles under various physiological conditions in animals, including ecdysozoans. During development, BMs undergo alterations through diverse intrinsic and extrinsic regulatory mechanisms; however, the full complement of pathways controlling these changes remain unclear. Here, we found that fat body-overexpression of <i>Drosophila miR-263b</i>, which is highly expressed during the larval-to-pupal transition, resulted in a decrease in the overall size of the larval fat body, and ultimately, in a severe growth defect accompanied by a reduction in cell proliferation and cell size. Interestingly, we further observed that a large proportion of the larval fat body cells were prematurely disassociated from each other. Moreover, we present evidence that miR-263b-5p suppresses the main component of BMs, <i>Laminin A</i> (<i>LanA</i>). Through experiments using RNA interference (RNAi) of <i>LanA</i>, we found that its depletion phenocopied the effects in <i>miR-263b</i>-overexpressing flies. Overall, our findings suggest a potential role for miR-263b in developmental growth and cell association by suppressing <i>LanA</i> expression in the <i>Drosophila</i> fat body.https://www.mdpi.com/2079-7737/12/8/1096<i>Drosophila</i>miR-263b<i>Laminin A</i>extracellular matrixbasement membranetissue remodeling |
| spellingShingle | Chae Jeong Kim Hyun Ho Kim Hee Kyung Kim Sojeong Lee Daegyu Jang Chanhyeok Kim Do-Hwan Lim MicroRNA miR-263b-5p Regulates Developmental Growth and Cell Association by Suppressing <i>Laminin A</i> in <i>Drosophila</i> Biology <i>Drosophila</i> miR-263b <i>Laminin A</i> extracellular matrix basement membrane tissue remodeling |
| title | MicroRNA miR-263b-5p Regulates Developmental Growth and Cell Association by Suppressing <i>Laminin A</i> in <i>Drosophila</i> |
| title_full | MicroRNA miR-263b-5p Regulates Developmental Growth and Cell Association by Suppressing <i>Laminin A</i> in <i>Drosophila</i> |
| title_fullStr | MicroRNA miR-263b-5p Regulates Developmental Growth and Cell Association by Suppressing <i>Laminin A</i> in <i>Drosophila</i> |
| title_full_unstemmed | MicroRNA miR-263b-5p Regulates Developmental Growth and Cell Association by Suppressing <i>Laminin A</i> in <i>Drosophila</i> |
| title_short | MicroRNA miR-263b-5p Regulates Developmental Growth and Cell Association by Suppressing <i>Laminin A</i> in <i>Drosophila</i> |
| title_sort | microrna mir 263b 5p regulates developmental growth and cell association by suppressing i laminin a i in i drosophila i |
| topic | <i>Drosophila</i> miR-263b <i>Laminin A</i> extracellular matrix basement membrane tissue remodeling |
| url | https://www.mdpi.com/2079-7737/12/8/1096 |
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