Sex chromosome differentiation in Humulus japonicus Siebold & Zuccarini, 1846 (Cannabaceae) revealed by fluorescence in situ hybridization of subtelomeric repeat
Humulus japonicus Siebold & Zucc (Japanese hop) is a dioecious species of the family Cannabaceae. The chromosome number is 2n = 16 = 14 + XX for females and 2n = 17 = 14 + XY1Y2 for male. To date, no fluorescence in situ hybridization (FISH) markers have been established for the identificati...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2012-07-01
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| Series: | Comparative Cytogenetics |
| Online Access: | http://compcytogen.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=1765 |
| _version_ | 1819315397161123840 |
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| author | Oleg Alexandrov Mikhail Divashuk Nikolay Yakovin Gennady Karlov |
| author_facet | Oleg Alexandrov Mikhail Divashuk Nikolay Yakovin Gennady Karlov |
| author_sort | Oleg Alexandrov |
| collection | DOAJ |
| description | Humulus japonicus Siebold & Zucc (Japanese hop) is a dioecious species of the family Cannabaceae. The chromosome number is 2n = 16 = 14 + XX for females and 2n = 17 = 14 + XY1Y2 for male. To date, no fluorescence in situ hybridization (FISH) markers have been established for the identification of H. japonicus sex chromosomes. In this paper, we report a method for the mitotic and meiotic sex chromosome differentiation in H. japonicus by FISH for HJSR, a high copy subtelomeric repeat. The signal is present in the subtelomeric region of one arm of the X chromosome. We demonstrate that males have two Y chromosomes that differ in FISH signal with the HJSR probe. Indeed, the HJSR probe hybridizes to a subtelomeric region on both arms of chromosome Y1 but not of chromosome Y2. The orientation and position of pseudoautosomal regions (PAR1 and PAR2) were also determined. |
| first_indexed | 2024-12-24T09:59:27Z |
| format | Article |
| id | doaj.art-f9e3cf8801db401aaada23ccd35707bd |
| institution | Directory Open Access Journal |
| issn | 1993-0771 1993-078X |
| language | English |
| last_indexed | 2024-12-24T09:59:27Z |
| publishDate | 2012-07-01 |
| publisher | Pensoft Publishers |
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| series | Comparative Cytogenetics |
| spelling | doaj.art-f9e3cf8801db401aaada23ccd35707bd2022-12-21T17:01:11ZengPensoft PublishersComparative Cytogenetics1993-07711993-078X2012-07-016323924710.3897/compcytogen.v6i3.32611765Sex chromosome differentiation in Humulus japonicus Siebold & Zuccarini, 1846 (Cannabaceae) revealed by fluorescence in situ hybridization of subtelomeric repeatOleg AlexandrovMikhail DivashukNikolay YakovinGennady KarlovHumulus japonicus Siebold & Zucc (Japanese hop) is a dioecious species of the family Cannabaceae. The chromosome number is 2n = 16 = 14 + XX for females and 2n = 17 = 14 + XY1Y2 for male. To date, no fluorescence in situ hybridization (FISH) markers have been established for the identification of H. japonicus sex chromosomes. In this paper, we report a method for the mitotic and meiotic sex chromosome differentiation in H. japonicus by FISH for HJSR, a high copy subtelomeric repeat. The signal is present in the subtelomeric region of one arm of the X chromosome. We demonstrate that males have two Y chromosomes that differ in FISH signal with the HJSR probe. Indeed, the HJSR probe hybridizes to a subtelomeric region on both arms of chromosome Y1 but not of chromosome Y2. The orientation and position of pseudoautosomal regions (PAR1 and PAR2) were also determined.http://compcytogen.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=1765 |
| spellingShingle | Oleg Alexandrov Mikhail Divashuk Nikolay Yakovin Gennady Karlov Sex chromosome differentiation in Humulus japonicus Siebold & Zuccarini, 1846 (Cannabaceae) revealed by fluorescence in situ hybridization of subtelomeric repeat Comparative Cytogenetics |
| title | Sex chromosome differentiation in Humulus japonicus Siebold & Zuccarini, 1846 (Cannabaceae) revealed by fluorescence in situ hybridization of subtelomeric repeat |
| title_full | Sex chromosome differentiation in Humulus japonicus Siebold & Zuccarini, 1846 (Cannabaceae) revealed by fluorescence in situ hybridization of subtelomeric repeat |
| title_fullStr | Sex chromosome differentiation in Humulus japonicus Siebold & Zuccarini, 1846 (Cannabaceae) revealed by fluorescence in situ hybridization of subtelomeric repeat |
| title_full_unstemmed | Sex chromosome differentiation in Humulus japonicus Siebold & Zuccarini, 1846 (Cannabaceae) revealed by fluorescence in situ hybridization of subtelomeric repeat |
| title_short | Sex chromosome differentiation in Humulus japonicus Siebold & Zuccarini, 1846 (Cannabaceae) revealed by fluorescence in situ hybridization of subtelomeric repeat |
| title_sort | sex chromosome differentiation in humulus japonicus siebold zuccarini 1846 cannabaceae revealed by fluorescence in situ hybridization of subtelomeric repeat |
| url | http://compcytogen.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=1765 |
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