Genetic diversity of Bartonella species in small mammals in the Qaidam Basin, western China
Abstract Investigation of the prevalence and diversity of Bartonella infections in small mammals in the Qaidam Basin, western China, could provide a scientific basis for the control and prevention of Bartonella infections in humans. Accordingly, in this study, small mammals were captured using snap...
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| Format: | Article |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-81508-w |
| _version_ | 1818400715695980544 |
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| author | Huaxiang Rao Shoujiang Li Liang Lu Rong Wang Xiuping Song Kai Sun Yan Shi Dongmei Li Juan Yu |
| author_facet | Huaxiang Rao Shoujiang Li Liang Lu Rong Wang Xiuping Song Kai Sun Yan Shi Dongmei Li Juan Yu |
| author_sort | Huaxiang Rao |
| collection | DOAJ |
| description | Abstract Investigation of the prevalence and diversity of Bartonella infections in small mammals in the Qaidam Basin, western China, could provide a scientific basis for the control and prevention of Bartonella infections in humans. Accordingly, in this study, small mammals were captured using snap traps in Wulan County and Ge’ermu City, Qaidam Basin, China. Spleen and brain tissues were collected and cultured to isolate Bartonella strains. The suspected positive colonies were detected with polymerase chain reaction amplification and sequencing of gltA, ftsZ, RNA polymerase beta subunit (rpoB) and ribC genes. Among 101 small mammals, 39 were positive for Bartonella, with the infection rate of 38.61%. The infection rate in different tissues (spleens and brains) (χ 2 = 0.112, P = 0.738) and gender (χ 2 = 1.927, P = 0.165) of small mammals did not have statistical difference, but that in different habitats had statistical difference (χ 2 = 10.361, P = 0.016). Through genetic evolution analysis, 40 Bartonella strains were identified (two different Bartonella species were detected in one small mammal), including B. grahamii (30), B. jaculi (3), B. krasnovii (3) and Candidatus B. gerbillinarum (4), which showed rodent-specific characteristics. B. grahamii was the dominant epidemic strain (accounted for 75.0%). Furthermore, phylogenetic analysis showed that B. grahamii in the Qaidam Basin, might be close to the strains isolated from Japan and China. Overall, we observed a high prevalence of Bartonella infection in small mammals in the Qaidam Basin. B. grahamii may cause human disease, and the pathogenicity of the others Bartonella species needs further study, the corresponding prevention and control measures should be taken into consideration. |
| first_indexed | 2024-12-14T07:40:59Z |
| format | Article |
| id | doaj.art-f9e5cb1246724d7db4bee3860b4235a9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T07:40:59Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f9e5cb1246724d7db4bee3860b4235a92022-12-21T23:11:03ZengNature PortfolioScientific Reports2045-23222021-01-0111111010.1038/s41598-021-81508-wGenetic diversity of Bartonella species in small mammals in the Qaidam Basin, western ChinaHuaxiang Rao0Shoujiang Li1Liang Lu2Rong Wang3Xiuping Song4Kai Sun5Yan Shi6Dongmei Li7Juan Yu8Department of Public Health and Preventive Medicine, Changzhi Medical CollegeInstitute for Communicable Disease Control and Prevention, Qinghai Center for Disease Control and PreventionState Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionInstitute for Communicable Disease Control and Prevention, Qinghai Center for Disease Control and PreventionState Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionWulan Center for Disease Control and PreventionInstitute for Communicable Disease Control and Prevention, Qinghai Center for Disease Control and PreventionState Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionDepartment of Basic Medical Sciences, Changzhi Medical CollegeAbstract Investigation of the prevalence and diversity of Bartonella infections in small mammals in the Qaidam Basin, western China, could provide a scientific basis for the control and prevention of Bartonella infections in humans. Accordingly, in this study, small mammals were captured using snap traps in Wulan County and Ge’ermu City, Qaidam Basin, China. Spleen and brain tissues were collected and cultured to isolate Bartonella strains. The suspected positive colonies were detected with polymerase chain reaction amplification and sequencing of gltA, ftsZ, RNA polymerase beta subunit (rpoB) and ribC genes. Among 101 small mammals, 39 were positive for Bartonella, with the infection rate of 38.61%. The infection rate in different tissues (spleens and brains) (χ 2 = 0.112, P = 0.738) and gender (χ 2 = 1.927, P = 0.165) of small mammals did not have statistical difference, but that in different habitats had statistical difference (χ 2 = 10.361, P = 0.016). Through genetic evolution analysis, 40 Bartonella strains were identified (two different Bartonella species were detected in one small mammal), including B. grahamii (30), B. jaculi (3), B. krasnovii (3) and Candidatus B. gerbillinarum (4), which showed rodent-specific characteristics. B. grahamii was the dominant epidemic strain (accounted for 75.0%). Furthermore, phylogenetic analysis showed that B. grahamii in the Qaidam Basin, might be close to the strains isolated from Japan and China. Overall, we observed a high prevalence of Bartonella infection in small mammals in the Qaidam Basin. B. grahamii may cause human disease, and the pathogenicity of the others Bartonella species needs further study, the corresponding prevention and control measures should be taken into consideration.https://doi.org/10.1038/s41598-021-81508-w |
| spellingShingle | Huaxiang Rao Shoujiang Li Liang Lu Rong Wang Xiuping Song Kai Sun Yan Shi Dongmei Li Juan Yu Genetic diversity of Bartonella species in small mammals in the Qaidam Basin, western China Scientific Reports |
| title | Genetic diversity of Bartonella species in small mammals in the Qaidam Basin, western China |
| title_full | Genetic diversity of Bartonella species in small mammals in the Qaidam Basin, western China |
| title_fullStr | Genetic diversity of Bartonella species in small mammals in the Qaidam Basin, western China |
| title_full_unstemmed | Genetic diversity of Bartonella species in small mammals in the Qaidam Basin, western China |
| title_short | Genetic diversity of Bartonella species in small mammals in the Qaidam Basin, western China |
| title_sort | genetic diversity of bartonella species in small mammals in the qaidam basin western china |
| url | https://doi.org/10.1038/s41598-021-81508-w |
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