The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish Virology
Much of the knowledge on viruses is focused on those that can be propagated using cell-cultures or that can cause disease in humans or in economically important animals and plants. However, this only reflects a small portion of the virosphere. Therefore, in this study, we explore by targeted next-ge...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/11/1634 |
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| author | Andreia Filipa-Silva Ricardo Parreira Sandra Martínez-Puchol Sílvia Bofill-Mas Maria Teresa Barreto Crespo Mónica Nunes |
| author_facet | Andreia Filipa-Silva Ricardo Parreira Sandra Martínez-Puchol Sílvia Bofill-Mas Maria Teresa Barreto Crespo Mónica Nunes |
| author_sort | Andreia Filipa-Silva |
| collection | DOAJ |
| description | Much of the knowledge on viruses is focused on those that can be propagated using cell-cultures or that can cause disease in humans or in economically important animals and plants. However, this only reflects a small portion of the virosphere. Therefore, in this study, we explore by targeted next-generation sequencing, how the virome varies between Atlantic horse mackerels and gilthead seabreams from fisheries and aquaculture from the center and south regions of Portugal. Viral genomes potentially pathogenic to fish and crustaceans, as well as to humans, were identified, namely <i>Astroviridae</i>, <i>Nodaviridae</i>, <i>Hepadnaviridae</i>, <i>Birnaviridae</i>, <i>Caliciviridae</i>, and <i>Picornaviridae</i> families. Also bacteriophages sequences were identified corresponding to the majority of sequences detected, with <i>Myoviridae</i>, <i>Podoviridae</i>, and <i>Siphoviridae</i>, the most widespread families in both fish species. However, these findings can also be due to the presence of bacteria in fish tissues, or even to contamination. Overall, seabreams harbored viruses from a smaller number of families in comparison with mackerels. Therefore, the obtained data show that fish sold for consumption can harbor a high diversity of viruses, many of which are unknown, reflecting the overall uncharacterized virome of fish. While cross-species transmission of <i>bonafide</i> fish viruses to humans is unlikely, the finding of human pathogenic viruses in fish suggest that fish virome can be a potential threat regarding food safety. |
| first_indexed | 2024-03-10T15:00:22Z |
| format | Article |
| id | doaj.art-f9e7241d47164a4183b3adf6a211859a |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T15:00:22Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-f9e7241d47164a4183b3adf6a211859a2023-11-20T20:16:21ZengMDPI AGFoods2304-81582020-11-01911163410.3390/foods9111634The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish VirologyAndreia Filipa-Silva0Ricardo Parreira1Sandra Martínez-Puchol2Sílvia Bofill-Mas3Maria Teresa Barreto Crespo4Mónica Nunes5ITQB NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, PortugalGlobal Health and Tropical Medicine (GHTM) Research Center, Unidade de Microbiologia Médica, Instituto de Higiene e Medicina Tropical (IHTM), Universidade Nova de Lisboa (NOVA), 1349-008 Lisboa, PortugalLaboratory of Viruses Contaminants of Water and Food, Genetics, Microbiology & Statistics Department, Universitat de Barcelona, 08028 Barcelona, Catalonia, SpainLaboratory of Viruses Contaminants of Water and Food, Genetics, Microbiology & Statistics Department, Universitat de Barcelona, 08028 Barcelona, Catalonia, SpainITQB NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, PortugalITQB NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, PortugalMuch of the knowledge on viruses is focused on those that can be propagated using cell-cultures or that can cause disease in humans or in economically important animals and plants. However, this only reflects a small portion of the virosphere. Therefore, in this study, we explore by targeted next-generation sequencing, how the virome varies between Atlantic horse mackerels and gilthead seabreams from fisheries and aquaculture from the center and south regions of Portugal. Viral genomes potentially pathogenic to fish and crustaceans, as well as to humans, were identified, namely <i>Astroviridae</i>, <i>Nodaviridae</i>, <i>Hepadnaviridae</i>, <i>Birnaviridae</i>, <i>Caliciviridae</i>, and <i>Picornaviridae</i> families. Also bacteriophages sequences were identified corresponding to the majority of sequences detected, with <i>Myoviridae</i>, <i>Podoviridae</i>, and <i>Siphoviridae</i>, the most widespread families in both fish species. However, these findings can also be due to the presence of bacteria in fish tissues, or even to contamination. Overall, seabreams harbored viruses from a smaller number of families in comparison with mackerels. Therefore, the obtained data show that fish sold for consumption can harbor a high diversity of viruses, many of which are unknown, reflecting the overall uncharacterized virome of fish. While cross-species transmission of <i>bonafide</i> fish viruses to humans is unlikely, the finding of human pathogenic viruses in fish suggest that fish virome can be a potential threat regarding food safety.https://www.mdpi.com/2304-8158/9/11/1634virome diversitymetagenomicsAtlantic horse mackerelgilthead seabreamhealth-threat |
| spellingShingle | Andreia Filipa-Silva Ricardo Parreira Sandra Martínez-Puchol Sílvia Bofill-Mas Maria Teresa Barreto Crespo Mónica Nunes The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish Virology Foods virome diversity metagenomics Atlantic horse mackerel gilthead seabream health-threat |
| title | The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish Virology |
| title_full | The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish Virology |
| title_fullStr | The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish Virology |
| title_full_unstemmed | The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish Virology |
| title_short | The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish Virology |
| title_sort | unexplored virome of two atlantic coast fish contribution of next generation sequencing to fish virology |
| topic | virome diversity metagenomics Atlantic horse mackerel gilthead seabream health-threat |
| url | https://www.mdpi.com/2304-8158/9/11/1634 |
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