Effect of Danhong injection on heart failure in rats evaluated by metabolomics
BackgroundHeart failure (HF) is characterized by reduced ventricular filling or ejection function due to organic or non-organic cardiovascular diseases. Danhong injection (DHI) is a medicinal material used clinically to treat HF for many years in China. Although prior research has shown that Danhong...
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2023.1259182/full |
| _version_ | 1797666815540723712 |
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| author | Lin Li Lin Li Lin Li Senjie Zhong Jiahao Ye Jiahao Ye Siyuan Hu Zhixi Hu Zhixi Hu |
| author_facet | Lin Li Lin Li Lin Li Senjie Zhong Jiahao Ye Jiahao Ye Siyuan Hu Zhixi Hu Zhixi Hu |
| author_sort | Lin Li |
| collection | DOAJ |
| description | BackgroundHeart failure (HF) is characterized by reduced ventricular filling or ejection function due to organic or non-organic cardiovascular diseases. Danhong injection (DHI) is a medicinal material used clinically to treat HF for many years in China. Although prior research has shown that Danhong injection can improve cardiac function and structure, the biological mechanism has yet to be determined.MethodsSerum metabolic analysis was conducted via ultra-high-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UHPLC-QE/MS) to explore underlying protective mechanisms of DHI in the transverse aortic constriction (TAC)-induced heart failure. Multivariate statistical techniques were used in the research, such as unsupervised principal component analysis (PCA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA). MetaboAnalyst and Kyoto Encyclopedia of Genes and Genomes (KEGG) were employed to pinpoint pertinent metabolic pathways.ResultsAfter DHI treatment, cardiac morphology and function as well as the metabolism in model rats were improved. We identified 17 differential metabolites and six metabolic pathways. Two biomarkers, PC(18:3(6Z,9Z,12Z)/24:0) and L-Phenylalanine, were identified for the first time as strong indicators for the significant effect of DHI.ConclusionThis study revealed that DHI could regulate potential biomarkers and correlated metabolic pathway, which highlighted therapeutic potential of DHI in managing HF. |
| first_indexed | 2024-03-11T20:05:00Z |
| format | Article |
| id | doaj.art-f9e880bd8c714c4b998549dd0d8077af |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-03-11T20:05:00Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-f9e880bd8c714c4b998549dd0d8077af2023-10-04T08:15:28ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2023-10-011010.3389/fmed.2023.12591821259182Effect of Danhong injection on heart failure in rats evaluated by metabolomicsLin Li0Lin Li1Lin Li2Senjie Zhong3Jiahao Ye4Jiahao Ye5Siyuan Hu6Zhixi Hu7Zhixi Hu8The Domestic First-class Discipline Construction Project of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, ChinaProvincial Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan, ChinaHunan Engineering Technology Research Center for Medicinal and Functional Food, Changsha, Hunan, ChinaThe First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaThe First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaPost-Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan, ChinaThe Domestic First-class Discipline Construction Project of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, ChinaThe Domestic First-class Discipline Construction Project of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, ChinaProvincial Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan, ChinaBackgroundHeart failure (HF) is characterized by reduced ventricular filling or ejection function due to organic or non-organic cardiovascular diseases. Danhong injection (DHI) is a medicinal material used clinically to treat HF for many years in China. Although prior research has shown that Danhong injection can improve cardiac function and structure, the biological mechanism has yet to be determined.MethodsSerum metabolic analysis was conducted via ultra-high-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UHPLC-QE/MS) to explore underlying protective mechanisms of DHI in the transverse aortic constriction (TAC)-induced heart failure. Multivariate statistical techniques were used in the research, such as unsupervised principal component analysis (PCA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA). MetaboAnalyst and Kyoto Encyclopedia of Genes and Genomes (KEGG) were employed to pinpoint pertinent metabolic pathways.ResultsAfter DHI treatment, cardiac morphology and function as well as the metabolism in model rats were improved. We identified 17 differential metabolites and six metabolic pathways. Two biomarkers, PC(18:3(6Z,9Z,12Z)/24:0) and L-Phenylalanine, were identified for the first time as strong indicators for the significant effect of DHI.ConclusionThis study revealed that DHI could regulate potential biomarkers and correlated metabolic pathway, which highlighted therapeutic potential of DHI in managing HF.https://www.frontiersin.org/articles/10.3389/fmed.2023.1259182/fullheart failureDanhong injectionmetabolomicstransverse aortic constrictionChinese medicine (CM) |
| spellingShingle | Lin Li Lin Li Lin Li Senjie Zhong Jiahao Ye Jiahao Ye Siyuan Hu Zhixi Hu Zhixi Hu Effect of Danhong injection on heart failure in rats evaluated by metabolomics Frontiers in Medicine heart failure Danhong injection metabolomics transverse aortic constriction Chinese medicine (CM) |
| title | Effect of Danhong injection on heart failure in rats evaluated by metabolomics |
| title_full | Effect of Danhong injection on heart failure in rats evaluated by metabolomics |
| title_fullStr | Effect of Danhong injection on heart failure in rats evaluated by metabolomics |
| title_full_unstemmed | Effect of Danhong injection on heart failure in rats evaluated by metabolomics |
| title_short | Effect of Danhong injection on heart failure in rats evaluated by metabolomics |
| title_sort | effect of danhong injection on heart failure in rats evaluated by metabolomics |
| topic | heart failure Danhong injection metabolomics transverse aortic constriction Chinese medicine (CM) |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2023.1259182/full |
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