Staphylococcus aureus from Atopic Dermatitis Patients: Its Genetic Structure and Susceptibility to Phototreatment
ABSTRACT We characterized the population of Staphylococcus aureus from patients with atopic dermatitis (AD) in terms of (i) genetic diversity, (ii) presence and functionality of genes encoding important virulence factors: staphylococcal enterotoxins (sea, seb, sec, sed), toxic shock syndrome 1 toxin...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-06-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.04598-22 |
| _version_ | 1797803433414098944 |
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| author | Patrycja Ogonowska Klaudia Szymczak Joanna Empel Małgorzata Urbaś Agata Woźniak-Pawlikowska Wioletta Barańska-Rybak Dariusz Świetlik Joanna Nakonieczna |
| author_facet | Patrycja Ogonowska Klaudia Szymczak Joanna Empel Małgorzata Urbaś Agata Woźniak-Pawlikowska Wioletta Barańska-Rybak Dariusz Świetlik Joanna Nakonieczna |
| author_sort | Patrycja Ogonowska |
| collection | DOAJ |
| description | ABSTRACT We characterized the population of Staphylococcus aureus from patients with atopic dermatitis (AD) in terms of (i) genetic diversity, (ii) presence and functionality of genes encoding important virulence factors: staphylococcal enterotoxins (sea, seb, sec, sed), toxic shock syndrome 1 toxin (tsst-1), and Panton-Valentine leukocidin (lukS/lukF-PV) by spa typing, PCR, drug resistance profile determination, and Western blot. We then subjected the studied population of S. aureus to photoinactivation based on a light-activated compound called rose bengal (RB) to verify photoinactivation as an approach to effectively kill toxin-producing S. aureus. We have obtained 43 different spa types that can be grouped into 12 clusters, indicating for the first-time clonal complex (CC) 7 as the most widespread. A total of 65% of the tested isolates had at least one gene encoding the tested virulence factor, but their distribution differed between the group of children and adults, and between patients with AD and the control group without atopy. We detected a 3.5% frequency of methicillin-resistant strains (MRSA) and no other multidrug resistance. Despite genetic diversity and production of various toxins, all isolates tested were effectively photoinactivated (bacterial cell viability reduction ≥ 3 log10 units) under safe conditions for the human keratinocyte cell line, which indicates that photoinactivation can be a good option in skin decolonization. IMPORTANCE Staphylococcus aureus massively colonizes the skin of patients with atopic dermatitis (AD). It is worth noting that the frequency of detection of multidrug-resistant S. aureus (MRSA) in AD patients is higher than the healthy population, which makes treatment much more difficult. Information about the specific genetic background of S. aureus accompanying and/or causing exacerbations of AD is of great importance from the point of view of epidemiological investigations and the development of possible treatment options. |
| first_indexed | 2024-03-13T05:21:49Z |
| format | Article |
| id | doaj.art-f9e9cfa81dc14b1caa41522407203583 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-13T05:21:49Z |
| publishDate | 2023-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-f9e9cfa81dc14b1caa415224072035832023-06-15T13:18:31ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-06-0111310.1128/spectrum.04598-22Staphylococcus aureus from Atopic Dermatitis Patients: Its Genetic Structure and Susceptibility to PhototreatmentPatrycja Ogonowska0Klaudia Szymczak1Joanna Empel2Małgorzata Urbaś3Agata Woźniak-Pawlikowska4Wioletta Barańska-Rybak5Dariusz Świetlik6Joanna Nakonieczna7Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, PolandIntercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, PolandDepartment of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, PolandDepartment of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, PolandIntercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, PolandDepartment of Dermatology, Venereology and Allergology, Medical University of Gdańsk, Gdańsk, PolandDivision of Biostatistics and Neural Networks, Medical University of Gdańsk, Gdańsk, PolandIntercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, PolandABSTRACT We characterized the population of Staphylococcus aureus from patients with atopic dermatitis (AD) in terms of (i) genetic diversity, (ii) presence and functionality of genes encoding important virulence factors: staphylococcal enterotoxins (sea, seb, sec, sed), toxic shock syndrome 1 toxin (tsst-1), and Panton-Valentine leukocidin (lukS/lukF-PV) by spa typing, PCR, drug resistance profile determination, and Western blot. We then subjected the studied population of S. aureus to photoinactivation based on a light-activated compound called rose bengal (RB) to verify photoinactivation as an approach to effectively kill toxin-producing S. aureus. We have obtained 43 different spa types that can be grouped into 12 clusters, indicating for the first-time clonal complex (CC) 7 as the most widespread. A total of 65% of the tested isolates had at least one gene encoding the tested virulence factor, but their distribution differed between the group of children and adults, and between patients with AD and the control group without atopy. We detected a 3.5% frequency of methicillin-resistant strains (MRSA) and no other multidrug resistance. Despite genetic diversity and production of various toxins, all isolates tested were effectively photoinactivated (bacterial cell viability reduction ≥ 3 log10 units) under safe conditions for the human keratinocyte cell line, which indicates that photoinactivation can be a good option in skin decolonization. IMPORTANCE Staphylococcus aureus massively colonizes the skin of patients with atopic dermatitis (AD). It is worth noting that the frequency of detection of multidrug-resistant S. aureus (MRSA) in AD patients is higher than the healthy population, which makes treatment much more difficult. Information about the specific genetic background of S. aureus accompanying and/or causing exacerbations of AD is of great importance from the point of view of epidemiological investigations and the development of possible treatment options.https://journals.asm.org/doi/10.1128/spectrum.04598-22atopic eczemaMRSA/MSSAspa typeclonal complexsuperantigen (SAg)staphylococcal enterotoxin |
| spellingShingle | Patrycja Ogonowska Klaudia Szymczak Joanna Empel Małgorzata Urbaś Agata Woźniak-Pawlikowska Wioletta Barańska-Rybak Dariusz Świetlik Joanna Nakonieczna Staphylococcus aureus from Atopic Dermatitis Patients: Its Genetic Structure and Susceptibility to Phototreatment Microbiology Spectrum atopic eczema MRSA/MSSA spa type clonal complex superantigen (SAg) staphylococcal enterotoxin |
| title | Staphylococcus aureus from Atopic Dermatitis Patients: Its Genetic Structure and Susceptibility to Phototreatment |
| title_full | Staphylococcus aureus from Atopic Dermatitis Patients: Its Genetic Structure and Susceptibility to Phototreatment |
| title_fullStr | Staphylococcus aureus from Atopic Dermatitis Patients: Its Genetic Structure and Susceptibility to Phototreatment |
| title_full_unstemmed | Staphylococcus aureus from Atopic Dermatitis Patients: Its Genetic Structure and Susceptibility to Phototreatment |
| title_short | Staphylococcus aureus from Atopic Dermatitis Patients: Its Genetic Structure and Susceptibility to Phototreatment |
| title_sort | staphylococcus aureus from atopic dermatitis patients its genetic structure and susceptibility to phototreatment |
| topic | atopic eczema MRSA/MSSA spa type clonal complex superantigen (SAg) staphylococcal enterotoxin |
| url | https://journals.asm.org/doi/10.1128/spectrum.04598-22 |
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