Fast determination of citreoviridin residues in rice using a monoclonal antibody-based immunochromatographic strip assay
Citreoviridin (CTV) is a toxic secondary metabolite that can be found in mouldy cereals. In this study, a sensitive and ethanol-tolerant anti-CTV monoclonal antibody (6B2) was produced with a 50% inhibition concentration of 4.1 ng/mL and then labelled using gold nanoparticle (GNP). Based on competit...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2020.1786670 |
| _version_ | 1819073056202555392 |
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| author | Yue Li Guohao Jin Liqiang Liu Jing Xiao Hua Kuang |
| author_facet | Yue Li Guohao Jin Liqiang Liu Jing Xiao Hua Kuang |
| author_sort | Yue Li |
| collection | DOAJ |
| description | Citreoviridin (CTV) is a toxic secondary metabolite that can be found in mouldy cereals. In this study, a sensitive and ethanol-tolerant anti-CTV monoclonal antibody (6B2) was produced with a 50% inhibition concentration of 4.1 ng/mL and then labelled using gold nanoparticle (GNP). Based on competitive inhibition of the binding of CTV-COOH-BSA conjugate sprayed onto a membrane to limited GNP-labelled antibody by free CTV in the samples, an easy-to-operate immunochromatographic strip (ICS) was developed for the fast screening of CTV in rice. The results are seen as a colour change within 5-10 min. We used relatively safe ethanol instead of highly toxic methanol or chloroform as the extraction solution and found a visible detection limit of 100 μg/kg and cut-off value of 1000 μg/kg. The developed ICS assay worked well and represented a reliable, safe, fast and a highthroughput method for the detection of CTV residues in rice samples. |
| first_indexed | 2024-12-21T17:47:33Z |
| format | Article |
| id | doaj.art-f9ed97a0005c4dd9a0f66e6b0fb5ef18 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-21T17:47:33Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-f9ed97a0005c4dd9a0f66e6b0fb5ef182022-12-21T18:55:28ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432020-01-0131189390610.1080/09540105.2020.17866701786670Fast determination of citreoviridin residues in rice using a monoclonal antibody-based immunochromatographic strip assayYue Li0Guohao Jin1Liqiang Liu2Jing Xiao3Hua Kuang4State Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityNHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk AssessmentState Key Laboratory of Food Science and Technology, Jiangnan UniversityCitreoviridin (CTV) is a toxic secondary metabolite that can be found in mouldy cereals. In this study, a sensitive and ethanol-tolerant anti-CTV monoclonal antibody (6B2) was produced with a 50% inhibition concentration of 4.1 ng/mL and then labelled using gold nanoparticle (GNP). Based on competitive inhibition of the binding of CTV-COOH-BSA conjugate sprayed onto a membrane to limited GNP-labelled antibody by free CTV in the samples, an easy-to-operate immunochromatographic strip (ICS) was developed for the fast screening of CTV in rice. The results are seen as a colour change within 5-10 min. We used relatively safe ethanol instead of highly toxic methanol or chloroform as the extraction solution and found a visible detection limit of 100 μg/kg and cut-off value of 1000 μg/kg. The developed ICS assay worked well and represented a reliable, safe, fast and a highthroughput method for the detection of CTV residues in rice samples.http://dx.doi.org/10.1080/09540105.2020.1786670citreoviridinimmunochromatographic stripgold nanoparticlesmonoclonal antibodyrice |
| spellingShingle | Yue Li Guohao Jin Liqiang Liu Jing Xiao Hua Kuang Fast determination of citreoviridin residues in rice using a monoclonal antibody-based immunochromatographic strip assay Food and Agricultural Immunology citreoviridin immunochromatographic strip gold nanoparticles monoclonal antibody rice |
| title | Fast determination of citreoviridin residues in rice using a monoclonal antibody-based immunochromatographic strip assay |
| title_full | Fast determination of citreoviridin residues in rice using a monoclonal antibody-based immunochromatographic strip assay |
| title_fullStr | Fast determination of citreoviridin residues in rice using a monoclonal antibody-based immunochromatographic strip assay |
| title_full_unstemmed | Fast determination of citreoviridin residues in rice using a monoclonal antibody-based immunochromatographic strip assay |
| title_short | Fast determination of citreoviridin residues in rice using a monoclonal antibody-based immunochromatographic strip assay |
| title_sort | fast determination of citreoviridin residues in rice using a monoclonal antibody based immunochromatographic strip assay |
| topic | citreoviridin immunochromatographic strip gold nanoparticles monoclonal antibody rice |
| url | http://dx.doi.org/10.1080/09540105.2020.1786670 |
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