Comparative Analysis of Cytokine Profiles in Cerebrospinal Fluid and Blood Serum in Patients with Acute and Subacute Spinal Cord Injury
Background: Cytokines are actively involved in the regulation of the inflammatory and immune responses and have crucial importance in the outcome of spinal cord injuries (SCIs). Examining more objective and representative indicators of the patient’s condition is still required to reveal the fundamen...
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MDPI AG
2023-09-01
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| Online Access: | https://www.mdpi.com/2227-9059/11/10/2641 |
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| author | Davran Sabirov Sergei Ogurcov Ilya Shulman Ilyas Kabdesh Ekaterina Garanina Albert Sufianov Albert Rizvanov Yana Mukhamedshina |
| author_facet | Davran Sabirov Sergei Ogurcov Ilya Shulman Ilyas Kabdesh Ekaterina Garanina Albert Sufianov Albert Rizvanov Yana Mukhamedshina |
| author_sort | Davran Sabirov |
| collection | DOAJ |
| description | Background: Cytokines are actively involved in the regulation of the inflammatory and immune responses and have crucial importance in the outcome of spinal cord injuries (SCIs). Examining more objective and representative indicators of the patient’s condition is still required to reveal the fundamental patterns of the abovementioned posttraumatic processes, including the identification of changes in the expression of cytokines. Methods: We performed a dynamic (3, 7, and 14 days post-injury (dpi)) extended multiplex analysis of cytokine profiles in both CSF and blood serum of SCI patients with baseline American Spinal Injury Association Impairment Scale grades of A. Results: The data obtained showed a large elevation of IL6 (>58 fold) in CSF and IFN-γ (>14 fold) in blood serum at 3 dpi with a downward trend as the post-traumatic period increases. The level of cytokine CCL26 was significantly elevated in both CSF and blood serum at 3 days post-SCI, while other cytokines did not show the same trend in the different biosamples. Conclusions: The dynamic changes in cytokine levels observed in our study can explore the relationships with the SCI region and injury severity, paving the way for a better understanding of the pathophysiology of SCI and potentially more targeted and personalized therapeutic interventions. |
| first_indexed | 2024-03-10T21:26:01Z |
| format | Article |
| id | doaj.art-f9ee07a4d1344b698ae983e18429ca5b |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T21:26:01Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-f9ee07a4d1344b698ae983e18429ca5b2023-11-19T15:44:59ZengMDPI AGBiomedicines2227-90592023-09-011110264110.3390/biomedicines11102641Comparative Analysis of Cytokine Profiles in Cerebrospinal Fluid and Blood Serum in Patients with Acute and Subacute Spinal Cord InjuryDavran Sabirov0Sergei Ogurcov1Ilya Shulman2Ilyas Kabdesh3Ekaterina Garanina4Albert Sufianov5Albert Rizvanov6Yana Mukhamedshina7OpenLab “Gene and Cell Technologies”, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, RussiaNeurosurgical Department No. 2, Republic Clinical Hospital, 420138 Kazan, RussiaNeurosurgical Department No. 2, Republic Clinical Hospital, 420138 Kazan, RussiaOpenLab “Gene and Cell Technologies”, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, RussiaDepartment of Genetics, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, RussiaDepartment of Neurosurgery, Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), 119991 Moscow, RussiaOpenLab “Gene and Cell Technologies”, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, RussiaOpenLab “Gene and Cell Technologies”, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, RussiaBackground: Cytokines are actively involved in the regulation of the inflammatory and immune responses and have crucial importance in the outcome of spinal cord injuries (SCIs). Examining more objective and representative indicators of the patient’s condition is still required to reveal the fundamental patterns of the abovementioned posttraumatic processes, including the identification of changes in the expression of cytokines. Methods: We performed a dynamic (3, 7, and 14 days post-injury (dpi)) extended multiplex analysis of cytokine profiles in both CSF and blood serum of SCI patients with baseline American Spinal Injury Association Impairment Scale grades of A. Results: The data obtained showed a large elevation of IL6 (>58 fold) in CSF and IFN-γ (>14 fold) in blood serum at 3 dpi with a downward trend as the post-traumatic period increases. The level of cytokine CCL26 was significantly elevated in both CSF and blood serum at 3 days post-SCI, while other cytokines did not show the same trend in the different biosamples. Conclusions: The dynamic changes in cytokine levels observed in our study can explore the relationships with the SCI region and injury severity, paving the way for a better understanding of the pathophysiology of SCI and potentially more targeted and personalized therapeutic interventions.https://www.mdpi.com/2227-9059/11/10/2641traumatic spinal cord injurycytokine profilecerebrospinal fluidblood serummultiplex analysis |
| spellingShingle | Davran Sabirov Sergei Ogurcov Ilya Shulman Ilyas Kabdesh Ekaterina Garanina Albert Sufianov Albert Rizvanov Yana Mukhamedshina Comparative Analysis of Cytokine Profiles in Cerebrospinal Fluid and Blood Serum in Patients with Acute and Subacute Spinal Cord Injury Biomedicines traumatic spinal cord injury cytokine profile cerebrospinal fluid blood serum multiplex analysis |
| title | Comparative Analysis of Cytokine Profiles in Cerebrospinal Fluid and Blood Serum in Patients with Acute and Subacute Spinal Cord Injury |
| title_full | Comparative Analysis of Cytokine Profiles in Cerebrospinal Fluid and Blood Serum in Patients with Acute and Subacute Spinal Cord Injury |
| title_fullStr | Comparative Analysis of Cytokine Profiles in Cerebrospinal Fluid and Blood Serum in Patients with Acute and Subacute Spinal Cord Injury |
| title_full_unstemmed | Comparative Analysis of Cytokine Profiles in Cerebrospinal Fluid and Blood Serum in Patients with Acute and Subacute Spinal Cord Injury |
| title_short | Comparative Analysis of Cytokine Profiles in Cerebrospinal Fluid and Blood Serum in Patients with Acute and Subacute Spinal Cord Injury |
| title_sort | comparative analysis of cytokine profiles in cerebrospinal fluid and blood serum in patients with acute and subacute spinal cord injury |
| topic | traumatic spinal cord injury cytokine profile cerebrospinal fluid blood serum multiplex analysis |
| url | https://www.mdpi.com/2227-9059/11/10/2641 |
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