Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved]
Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of...
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| Format: | Article |
| Language: | English |
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Wellcome
2017-09-01
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| Series: | Wellcome Open Research |
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| Online Access: | https://wellcomeopenresearch.org/articles/2-80/v1 |
| _version_ | 1818272070294831104 |
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| author | Vu Thi Ty Hang Nguyen Thi Han Ny Tran My Phuc Pham Thi Thanh Tam Dang Thao Huong Ho Dang Trung Nghia Nguyen Tran Anh Vu Pham Thi Hong Phuong Nguyen Van Xang Nguyen Dong Pham Nhu Hiep Nguyen Van Hung Tran Tinh Hien Maia Rabaa Guy E. Thwaites Stephen Baker Le Van Tan H.Rogier van Doorn VIZIONS consortium |
| author_facet | Vu Thi Ty Hang Nguyen Thi Han Ny Tran My Phuc Pham Thi Thanh Tam Dang Thao Huong Ho Dang Trung Nghia Nguyen Tran Anh Vu Pham Thi Hong Phuong Nguyen Van Xang Nguyen Dong Pham Nhu Hiep Nguyen Van Hung Tran Tinh Hien Maia Rabaa Guy E. Thwaites Stephen Baker Le Van Tan H.Rogier van Doorn VIZIONS consortium |
| author_sort | Vu Thi Ty Hang |
| collection | DOAJ |
| description | Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription. |
| first_indexed | 2024-12-12T21:36:13Z |
| format | Article |
| id | doaj.art-f9ef2311a0e3446fbb93c0acae7de416 |
| institution | Directory Open Access Journal |
| issn | 2398-502X |
| language | English |
| last_indexed | 2024-12-12T21:36:13Z |
| publishDate | 2017-09-01 |
| publisher | Wellcome |
| record_format | Article |
| series | Wellcome Open Research |
| spelling | doaj.art-f9ef2311a0e3446fbb93c0acae7de4162022-12-22T00:11:11ZengWellcomeWellcome Open Research2398-502X2017-09-01210.12688/wellcomeopenres.12429.113459Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved]Vu Thi Ty Hang0Nguyen Thi Han Ny1Tran My Phuc2Pham Thi Thanh Tam3Dang Thao Huong4Ho Dang Trung Nghia5Nguyen Tran Anh Vu6Pham Thi Hong Phuong7Nguyen Van Xang8Nguyen Dong9Pham Nhu Hiep10Nguyen Van Hung11Tran Tinh Hien12Maia Rabaa13Guy E. Thwaites14Stephen Baker15Le Van Tan16H.Rogier van Doorn17VIZIONS consortiumHospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, VietnamHospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, VietnamHospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, VietnamHospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, VietnamHospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, VietnamHospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, VietnamDong Thap General Hospital, Dong Thap, VietnamDong Thap General Hospital, Dong Thap, VietnamKhanh Hoa General Hospital, Khanh Hoa, VietnamKhanh Hoa General Hospital, Khanh Hoa, VietnamHue Central Hospital, Thua Thien - Hue, VietnamDak Lak General Hospital, Dak Lak, VietnamHospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, VietnamHospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, VietnamHospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, VietnamHospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, VietnamHospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, VietnamCentre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UKBackground: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription.https://wellcomeopenresearch.org/articles/2-80/v1Methods for Diagnostic & Therapeutic StudiesVirology |
| spellingShingle | Vu Thi Ty Hang Nguyen Thi Han Ny Tran My Phuc Pham Thi Thanh Tam Dang Thao Huong Ho Dang Trung Nghia Nguyen Tran Anh Vu Pham Thi Hong Phuong Nguyen Van Xang Nguyen Dong Pham Nhu Hiep Nguyen Van Hung Tran Tinh Hien Maia Rabaa Guy E. Thwaites Stephen Baker Le Van Tan H.Rogier van Doorn VIZIONS consortium Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved] Wellcome Open Research Methods for Diagnostic & Therapeutic Studies Virology |
| title | Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved] |
| title_full | Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved] |
| title_fullStr | Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved] |
| title_full_unstemmed | Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved] |
| title_short | Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved] |
| title_sort | evaluation of the luminex xtag respiratory viral panel fast v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in vietnam version 1 referees 2 approved |
| topic | Methods for Diagnostic & Therapeutic Studies Virology |
| url | https://wellcomeopenresearch.org/articles/2-80/v1 |
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