High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry
Abstract The progression of prostate cancer (PCa) leads to poor prognosis. However, the molecular mechanism of PCa is still not completely clear. This study aimed to elucidate the important role of centromere protein A (CENPA) in PCa. Large numbers of bulk RNA sequencing (RNA‐seq) data and in‐house...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-10-01
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| Series: | IET Systems Biology |
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| Online Access: | https://doi.org/10.1049/syb2.12073 |
| _version_ | 1827792620395429888 |
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| author | Fang‐Cheng Jiang Gao‐Qiang Zhai Jia‐Lin Liu Rui‐Gong Wang Yuan‐Ping Yang Harivignesh Murugesan Xiao‐Xiang Yu Xiu‐Fang Du Juan He Zhen‐Bo Feng Shang Ling Pan Gang Chen Sheng‐Hua Li Zhi‐Guang Huang |
| author_facet | Fang‐Cheng Jiang Gao‐Qiang Zhai Jia‐Lin Liu Rui‐Gong Wang Yuan‐Ping Yang Harivignesh Murugesan Xiao‐Xiang Yu Xiu‐Fang Du Juan He Zhen‐Bo Feng Shang Ling Pan Gang Chen Sheng‐Hua Li Zhi‐Guang Huang |
| author_sort | Fang‐Cheng Jiang |
| collection | DOAJ |
| description | Abstract The progression of prostate cancer (PCa) leads to poor prognosis. However, the molecular mechanism of PCa is still not completely clear. This study aimed to elucidate the important role of centromere protein A (CENPA) in PCa. Large numbers of bulk RNA sequencing (RNA‐seq) data and in‐house immunohistochemistry data were used in analysing the expression level of CENPA in PCa and metastatic PCa (MPCa). Single‐cell RNA‐seq data was used to explore the expression status of CENPA in different prostate subpopulations. Enrichment analysis was employed to detect the function of CENPA in PCa. Clinicopathological parameters analysis was utilised in analysing the clinical value of CENPA. The results showed that CENPA was upregulated in PCa (standardised mean difference [SMD] = 0.83, p = 0.001) and MPCa (SMD = 0.61, p = 0.029). CENPA was overexpressed in prostate cancer stem cells (CSCs) with androgen receptor (AR) negative compared to epithelial cells with AR positive. CENPA may influence the development of PCa through affecting cell cycle. Patients with nodal metastasis had higher expression level of CENPA. And patients with high CENPA expression had poor disease‐free survival. Taken together, Overexpression of CENPA may influence the development of PCa by regulating cell cycle and promoting metastasis. |
| first_indexed | 2024-03-11T18:05:41Z |
| format | Article |
| id | doaj.art-f9f4979522174a3ebe8f3b6a7a7b20af |
| institution | Directory Open Access Journal |
| issn | 1751-8849 1751-8857 |
| language | English |
| last_indexed | 2024-03-11T18:05:41Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Systems Biology |
| spelling | doaj.art-f9f4979522174a3ebe8f3b6a7a7b20af2023-10-17T08:16:38ZengWileyIET Systems Biology1751-88491751-88572023-10-0117524525810.1049/syb2.12073High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistryFang‐Cheng Jiang0Gao‐Qiang Zhai1Jia‐Lin Liu2Rui‐Gong Wang3Yuan‐Ping Yang4Harivignesh Murugesan5Xiao‐Xiang Yu6Xiu‐Fang Du7Juan He8Zhen‐Bo Feng9Shang Ling Pan10Gang Chen11Sheng‐Hua Li12Zhi‐Guang Huang13Department of Pathology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Urology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Urology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Urology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Pathology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Pathology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Urology The 923rd Hospital of Chinese People's Liberation Army Joint Logistics Support Force Nanning ChinaDepartment of Pathology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Pathology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Pathology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Pathophysiology School of Pre‐clinical Medicine Guangxi Medical University Nanning Guangxi ChinaDepartment of Pathology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Urology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Pathology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaAbstract The progression of prostate cancer (PCa) leads to poor prognosis. However, the molecular mechanism of PCa is still not completely clear. This study aimed to elucidate the important role of centromere protein A (CENPA) in PCa. Large numbers of bulk RNA sequencing (RNA‐seq) data and in‐house immunohistochemistry data were used in analysing the expression level of CENPA in PCa and metastatic PCa (MPCa). Single‐cell RNA‐seq data was used to explore the expression status of CENPA in different prostate subpopulations. Enrichment analysis was employed to detect the function of CENPA in PCa. Clinicopathological parameters analysis was utilised in analysing the clinical value of CENPA. The results showed that CENPA was upregulated in PCa (standardised mean difference [SMD] = 0.83, p = 0.001) and MPCa (SMD = 0.61, p = 0.029). CENPA was overexpressed in prostate cancer stem cells (CSCs) with androgen receptor (AR) negative compared to epithelial cells with AR positive. CENPA may influence the development of PCa through affecting cell cycle. Patients with nodal metastasis had higher expression level of CENPA. And patients with high CENPA expression had poor disease‐free survival. Taken together, Overexpression of CENPA may influence the development of PCa by regulating cell cycle and promoting metastasis.https://doi.org/10.1049/syb2.12073centromere protein A (CENPA)data miningimmunohistochemistryprostate cancer (PCa)single‐cell RNA sequencing |
| spellingShingle | Fang‐Cheng Jiang Gao‐Qiang Zhai Jia‐Lin Liu Rui‐Gong Wang Yuan‐Ping Yang Harivignesh Murugesan Xiao‐Xiang Yu Xiu‐Fang Du Juan He Zhen‐Bo Feng Shang Ling Pan Gang Chen Sheng‐Hua Li Zhi‐Guang Huang High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry IET Systems Biology centromere protein A (CENPA) data mining immunohistochemistry prostate cancer (PCa) single‐cell RNA sequencing |
| title | High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry |
| title_full | High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry |
| title_fullStr | High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry |
| title_full_unstemmed | High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry |
| title_short | High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry |
| title_sort | high expression of centromere protein a and its molecular mechanism and clinical significance in prostate cancer a study based on data mining and immunohistochemistry |
| topic | centromere protein A (CENPA) data mining immunohistochemistry prostate cancer (PCa) single‐cell RNA sequencing |
| url | https://doi.org/10.1049/syb2.12073 |
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